This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bose, B. Articles by Little, J. R. Search for Related Content PubMed PubMed Citation Articles by Bose, B. Articles by Little, J. R.

Stroke, Vol 19, 28-37, Copyright © 1988 by American Heart Association

ARTICLES

Evolving focal cerebral ischemia in cats: spatial correlation of nuclear magnetic resonance imaging, cerebral blood flow, tetrazolium staining, and histopathology

B Bose, SC Jones, R Lorig, HT Friel, M Weinstein and JR Little
Department of Neurological Surgery, Cleveland Clinic Foundation, OH 44106-4775.

The spatial correlation of nuclear magnetic resonance imaging (NMRI) and cerebral blood flow (CBF) may improve our ability to identify ischemic brain lesions and may provide further insight into the pathophysiology of early cerebral ischemia. Eleven pentobarbital- anesthetized adult cats underwent exposure of the common carotid arteries bilaterally and the right middle cerebral artery through a transorbital approach. Baseline NMRI images were obtained with a single spin-echo, multislice technique using a 0.6-T field, 0.4-cm slice thickness, and a surface coil. Focal ischemia was produced with right middle cerebral artery occlusion and potentiated with bilateral common carotid artery ligation. Sequential NMRI studies were then performed at 1, 2, 4, 6, and 12 hours or until CBF was determined in the same cats using [14C]iodoantipyrine at either 2 (n = 2), 4 (n = 2), 6 (n = 2), or 12 (n = 1) hours after the time of occlusion. This protocol allowed temporal and spatial correlation of NMRI and CBF. Alternate 5-mm brain slices were incubated with 1% 2,3,5-triphenyltetrazolium chloride (TTC) for 45 minutes at 37-41 degrees C and frozen in liquid Freon for later autoradiographic CBF determination. Four cats were studied only with NMRI and TTC (not CBF). The correlation between areas of increased NMRI signal intensity observed in T2-weighted images (repetition time 2,000 msec, echo time 120 msec), vital staining with TTC, low CBF, and routine histology was evaluated. During the early phase (less than 6 hours), T2-weighted NMRI changes were localized to the central ischemic gray matter areas, as defined in the later CBF images, with no involvement of the white matter. By the twelfth hour the NMRI changes involved the entire ischemic area including gray and white matter. The initial visible changes seen on T2-weighted NMRI are suggestive of cellular edema, and the later changes are characteristic of vasogenic edema. The spread of NMRI changes compared with the ischemic area determined from autoradiographic CBF is consistent with the previously described biphasic evolution of ischemic injury. These data suggest that T2-weighted NMRI could be used clinically to delineate areas of acute ischemic stroke.

This article has been cited by other articles:

				K. R. Thulborn, T. S. Gindin, D. Davis, and P. Erb Comprehensive MR Imaging Protocol for Stroke Management: Tissue Sodium Concentration as a Measure of Tissue Viability in Nonhuman Primate Studies and in Clinical Studies Radiology, October 1, 1999; 213(1): 156 - 166. [Abstract] [Full Text]

				A. M. Ulug, N. Beauchamp Jr, R. N. Bryan, and P. C.M. van Zijl Absolute Quantitation of Diffusion Constants in Human Stroke Stroke, March 1, 1997; 28(3): 483 - 490. [Abstract] [Full Text]

				A. R. Young, O. Touzani, J.-M. Derlon, G. Sette, E. T. MacKenzie, and J.-C. Baron Early Reperfusion in the Anesthetized Baboon Reduces Brain Damage Following Middle Cerebral Artery Occlusion : A Quantitative Analysis of Infarction Volume Stroke, March 1, 1997; 28(3): 632 - 638. [Abstract] [Full Text]

				K.M.A. Welch, J. Windham, R. A. Knight, V. Nagesh, J. W. Hugg, M. Jacobs, D. Peck, P. Booker, M. O. Dereski, and S. R. Levine A Model to Predict the Histopathology of Human Stroke Using Diffusion and T2-Weighted Magnetic Resonance Imaging Stroke, November 1, 1995; 26(11): 1983 - 1989. [Abstract] [Full Text]

				A. D. Perez-Trepichio, M. Xue, T. C. Ng, A. W. Majors, A. J. Furlan, I. A. Awad, and S. C. Jones Sensitivity of Magnetic Resonance Diffusion-Weighted Imaging and Regional Relationship Between the Apparent Diffusion Coefficient and Cerebral Blood Flow in Rat Focal Cerebral Ischemia Stroke, April 1, 1995; 26(4): 667 - 675. [Abstract] [Full Text]

				E. Kozniewska, T. P. L. Roberts, M. Tsuura, J. Mintorovitch, M. E. Moseley, and J. Kucharczyk NG-Nitro-L-Arginine Delays the Development of Brain Injury During Focal Ischemia in Rats Stroke, February 1, 1995; 26(2): 282 - 289. [Abstract] [Full Text]