Stroke, Vol 20, 1037-1043, Copyright © 1989 by American Heart Association
H Nagasawa and K Kogure
We describe a new focal ischemia model consisting of unilateral middle
cerebral artery occlusion with a silicone rubber cylinder attached to a
nylon surgical thread inserted through the internal carotid artery in rats.
Recirculation was accomplished by pulling the thread out of the artery. We
evaluated the reliability of this model and studied the influence of
reperfusion of the brain by measuring regional cerebral blood flow in 30
rats and by using conventional neuropathologic methods after different
periods of occlusion in 48 rats. The anterior neocortex and the lateral
part of the caudate putamen, which were supplied by the occluded middle
cerebral artery, were the regions most frequently damaged. After 1 hour of
occlusion in five rats, in the cortex supplied by the occluded artery mean
+/- SD blood flow was 0.19 +/- 0.08 ml/g/min (approximately 15% of that in
the corresponding region of five sham-operated control rats), and mild
scattered ischemic cell change was observed. Three (n = 5) or six (n = 5)
hours of occlusion reduced blood flow more severely and caused severe
ischemic cell changes in the cortex supplied by the occluded artery in
proportion to the duration of ischemia. Characteristically, in five rats
subjected to 3 hours of occlusion followed by 3 hours of recirculation,
blood flow was restored and spongy edematous change was observed in the
cortex supplied by the recirculated artery. This change resulted in
hypoperfusion of the neighboring cortical region surrounding the
recirculated area. Our model should be useful in various investigations of
the influence of reperfusion on focal ischemic brain injury.
ARTICLES
Correlation between cerebral blood flow and histologic changes in a new rat model of middle cerebral artery occlusion
Department of Neurology, Tohoku University, School of Medicine, Sendai, Japan.
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