This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Eke, A. Articles by Garcia, J. H. Search for Related Content PubMed PubMed Citation Articles by Eke, A. Articles by Garcia, J. H.

Stroke, Vol 21, 299-304, Copyright © 1990 by American Heart Association

ARTICLES

Histologic assessment of neurons in rat models of cerebral ischemia

A Eke, KA Conger, M Anderson and JH Garcia
Department of Pathology, University of Alabama, Birmingham 35294.

We describe a method for typing neurons into four progressive stages of ischemic deterioration based on visual characterization of the nucleus in terms of its optical contrast, delineation along the nuclear- cytoplasmic interface, and its shape. Difficulty in assessing nuclear shape required the introduction of an angularity comparator chart to improve the investigator's accuracy. Three investigators typed neurons obtained from normal, ischemic, and ischemic-reperfused rat brains. Accuracy and reproducibility of the investigators' typing decisions with and without the angularity comparator charts were evaluated. The accuracy of subjective shape assessment was compared with objective digitizer measurements of the same. The angularity comparator charts reduced subjective shape classification error by two thirds, and group error (overall performance expressed by the coefficient of variance) decreased from 15.9% to 4.7% for Type I (normal cells), from 33.9% to 17.3% for Type II (cells with angular nuclei), from 15.5% to 14.1% for Type III (cells with smeared nuclei), and from 3.2% to 5.5% for Type IV (dead cells). Thus, Type I and IV neurons can be assessed at a higher reproducibility than the intermediate Types II and III. Our typing method can also be used to evaluate the effect of treatment regimes on ischemic neuronal damage.

This article has been cited by other articles:

				T. Sairanen, M.-L. Karjalainen-Lindsberg, A. Paetau, P. Ijas, and P. J. Lindsberg Apoptosis dominant in the periinfarct area of human ischaemic stroke--a possible target of antiapoptotic treatments Brain, January 1, 2006; 129(1): 189 - 199. [Abstract] [Full Text] [PDF]

				N. J. Solenski, C. G. diPierro, P. A. Trimmer, A.-L. Kwan, and G. A. Helms Ultrastructural Changes of Neuronal Mitochondria After Transient and Permanent Cerebral Ischemia Stroke, March 1, 2002; 33(3): 816 - 824. [Abstract] [Full Text] [PDF]

				T. Sairanen, O. Carpen, M.-L. Karjalainen-Lindsberg, A. Paetau, U. Turpeinen, M. Kaste, P. J. Lindsberg, and D. F. Cechetto Evolution of Cerebral Tumor Necrosis Factor-{alpha} Production During Human Ischemic Stroke Editorial Comment Stroke, August 1, 2001; 32(8): 1750 - 1758. [Abstract] [Full Text] [PDF]

				K.-F. Liu, F. Li, T. Tatlisumak, J. H. Garcia, C. H. Sotak, M. Fisher, and J. D. Fenstermacher Regional Variations in the Apparent Diffusion Coefficient and the Intracellular Distribution of Water in Rat Brain During Acute Focal Ischemia Stroke, August 1, 2001; 32(8): 1897 - 1905. [Abstract] [Full Text] [PDF]

				J. Krupinski, R. Issa, T. Bujny, M. Slevin, P. Kumar, S. Kumar, and J. Kaluza A Putative Role for Platelet-Derived Growth Factor in Angiogenesis and Neuroprotection After Ischemic Stroke in Humans Stroke, March 1, 1997; 28(3): 564 - 573. [Abstract] [Full Text]

				P. J. Lindsberg, O. Carpen, A. Paetau, M.-L. Karjalainen-Lindsberg, and M. Kaste Endothelial ICAM-1 Expression Associated With Inflammatory Cell Response in Human Ischemic Stroke Circulation, September 1, 1996; 94(5): 939 - 945. [Abstract] [Full Text]

				G. F. Hamann, Y. Okada, R. Fitridge, and G. J. del Zoppo Microvascular Basal Lamina Antigens Disappear During Cerebral Ischemia and Reperfusion Stroke, November 1, 1995; 26(11): 2120 - 2126. [Abstract] [Full Text]

				A. Radovsky, P. Safar, F. Sterz, Y. Leonov, H. Reich, and K. Kuboyama Regional Prevalence and Distribution of Ischemic Neurons in Dog Brains 96 Hours After Cardiac Arrest of 0 to 20 Minutes Stroke, November 1, 1995; 26(11): 2127 - 2134. [Abstract] [Full Text]

				J. H. Garcia, S. Wagner, K.-F. Liu, and X.-j. Hu Neurological Deficit and Extent of Neuronal Necrosis Attributable to Middle Cerebral Artery Occlusion in Rats : Statistical Validation Stroke, April 1, 1995; 26(4): 627 - 635. [Abstract] [Full Text]