This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Maruyama, K. Articles by Yanagisawa, N. Search for Related Content PubMed PubMed Citation Articles by Maruyama, K. Articles by Yanagisawa, N.

Stroke, Vol 21, 397-403, Copyright © 1990 by American Heart Association

ARTICLES

Immunohistochemical characterization of cerebrovascular amyloid in 46 autopsied cases using antibodies to beta protein and cystatin C

K Maruyama, S Ikeda, T Ishihara, D Allsop and N Yanagisawa
Department of Medicine, Shinshu University School of Medicine, Matsumoto, Japan.

Using immunohistochemical staining methods with antibodies to amyloid beta protein and human cystatin C, we examined cerebrovascular amyloid protein in the brains from 46 cases with cerebral amyloid angiopathy (seven with Alzheimer's disease, one with Down's syndrome, 18 with intracranial hemorrhage, 10 with cerebral infarction, and 10 elderly patients without any neurologic disorder). All cerebrovascular amyloid deposits in these 46 cases were consistently immunoreactive to anti- beta protein antibody. However, in nine cases some vascular walls with strong beta protein immunoreactivity also reacted less intensely with the anti-cystatin C antiserum. Of these nine cases, seven showed relatively heavy cerebrovascular amyloid deposition, and all seven had suffered a fatal subcortical hemorrhage presumably caused by cerebral amyloid angiopathy. Previous limited studies have suggested that the amyloid protein seen in elderly individuals with cerebral amyloid angiopathy is composed of beta protein. However, subcortical hemorrhage rarely occurs in such individuals. Our study shows that aged patients with different brain disorders commonly suffer from beta protein-type cerebral amyloid angiopathy, and we also suggest that the severity of beta protein-type cerebrovascular amyloid deposition is a fundamental factor in cerebral amyloid angiopathy-induced brain hemorrhage in the elderly. The nature of the cystatin C-immunoreactive substance in some of these vascular lesions is uncertain, but it might conceivably play an additional important role in the pathogenesis of brain hemorrhage in these cases.

This article has been cited by other articles:

				J. Sundelof, J. Arnlov, E. Ingelsson, J. Sundstrom, S. Basu, B. Zethelius, A. Larsson, M. C. Irizarry, V. Giedraitis, E. Ronnemaa, et al. Serum cystatin C and the risk of Alzheimer disease in elderly men Neurology, September 30, 2008; 71(14): 1072 - 1079. [Abstract] [Full Text] [PDF]

				A. Deng, M. C. Irizarry, R. M. Nitsch, J. H. Growdon, and G. W. Rebeck Elevation of Cystatin C in Susceptible Neurons in Alzheimer's Disease Am. J. Pathol., September 1, 2001; 159(3): 1061 - 1068. [Abstract] [Full Text] [PDF]

				D. T. Winkler, L. Bondolfi, M. C. Herzig, L. Jann, M. E. Calhoun, K.-H. Wiederhold, M. Tolnay, M. Staufenbiel, and M. Jucker Spontaneous Hemorrhagic Stroke in a Mouse Model of Cerebral Amyloid Angiopathy J. Neurosci., March 1, 2001; 21(5): 1619 - 1627. [Abstract] [Full Text] [PDF]

				J. P. Melchor and W. E. Van Nostrand Fibrillar Amyloid beta -Protein Mediates the Pathologic Accumulation of Its Secreted Precursor in Human Cerebrovascular Smooth Muscle Cells J. Biol. Chem., March 24, 2000; 275(13): 9782 - 9791. [Abstract] [Full Text] [PDF]

				L. Wei, L. C. Walker, E. Levy, and W. I. Rosenblum Cystatin C: Icelandic-Like Mutation in an Animal Model of Cerebrovascular ß-Amyloidosis Stroke, November 1, 1996; 27(11): 2080 - 2085. [Abstract] [Full Text]

				C. Graffagnino, M. H. Herbstreith, D. E. Schmechel, E. Levy, A. D. Roses, and M. J. Alberts Cystatin C Mutation in an Elderly Man With Sporadic Amyloid Angiopathy and Intracerebral Hemorrhage Stroke, November 1, 1995; 26(11): 2190 - 2193. [Abstract] [Full Text]

				W. E. Van Nostrand, J. P. Melchor, H. S. Cho, S. M. Greenberg, and G. W. Rebeck Pathogenic Effects of D23N Iowa Mutant Amyloid beta -Protein J. Biol. Chem., August 24, 2001; 276(35): 32860 - 32866. [Abstract] [Full Text] [PDF]