Stroke, Vol 21, 801-807, Copyright © 1990 by American Heart Association
GA Rosenberg, S Mun-Bryce, M Wesley and M Kornfeld
Intracranial bleeding is an important cause of brain masses and edema. To
study the pathophysiology of intracerebral hemorrhage, we produced
experimental hemorrhages in 53 rats and characterized the lesion by
histology, brain water content, and behavior. Adult rats had 2 microliters
saline containing 0.5 unit bacterial collagenase infused into the left
caudate nucleus. Histologically, erythrocytes were seen around blood
vessels at the needle puncture site within the first hour. By 4 hours there
were hematomas, the size of which depended on the amount of collagenase
injected. Necrotic masses containing fluid, blood cells, and fibrin were
seen at 24 hours. Lipid-filled macrophages were observed at 7 days and
cysts at 3 weeks. Water content was significantly increased 4, 24, and 48
hours after infusion at the needle puncture site and for 24 hours in
posterior brain sections. Behavioral abnormalities were present for 48
hours, with recovery of function occurring during the first week. Brain
tissue contains Type IV collagen in the basal lamina. Collagenase, which
occurs in an inactive form in cells, is released and activated during
injury, leading to disruption of the extracellular matrix.
Collagenase-induced intracerebral hemorrhage is a reproducible animal model
for the study of the effects of the hematoma and brain edema.
ARTICLES
Collagenase-induced intracerebral hemorrhage in rats
Neurology Service, Veterans Affairs Medical Center, Albuquerque, New Mexico.
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