This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Oh, S. M. Articles by Betz, A. L. Search for Related Content PubMed PubMed Citation Articles by Oh, S. M. Articles by Betz, A. L.

Stroke, Vol 22, 915-921, Copyright © 1991 by American Heart Association

ARTICLES

Interaction between free radicals and excitatory amino acids in the formation of ischemic brain edema in rats

SM Oh and AL Betz
Department of Surgery (Neurosurgery), University of Michigan, Ann Arbor 48109-0718.

Both oxygen free radicals and excitatory amino acids have been implicated as important cellular toxins in ischemic brain. Recent in vitro studies suggest that there may be a mutual interaction between these two mediators. We explored the relation between oxygen free radicals and excitatory amino acids in the development of ischemic brain edema in vivo. Male Sprague-Dawley rats were treated with the free radical scavenger dimethylthiourea 1 hour before ischemia or with the excitotoxin antagonist MK-801 30 minutes before ischemia produced by occlusion of the middle cerebral artery. Groups of seven or eight animals were treated with vehicle, low-dose (375 mg/kg) dimethylthiourea, high-dose (750 mg/kg) dimethylthiourea, low-dose (0.5 mg/kg) MK-801, high-dose (2.0 mg/kg) MK-801, or both high-dose dimethylthiourea and low-dose MK-801. After 4 hours of ischemia, brain water content was determined. In eight vehicle-treated controls, mean +/- SEM water content of tissue in the center of the ischemic zone was 83.29 +/- 0.18%. A significant reduction of brain edema was observed in all drug-treated groups: for example, 50.2% (p less than 0.001) in the high-dose dimethylthiourea group, 53.7% (p less than 0.001) in the low- dose MK-801 group, and 66.4% (p less than 0.001) in the combined dimethylthiourea and MK-801 group. Combined treatment with dimethylthiourea and MK-801 provided no significant additive effect over that resulting from treatment with MK-801 alone.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				A. Davalos, J. Castillo, J. Marrugat, J. M. Fernandez-Real, A. Armengou, P. Cacabelos, and R. Rama Body iron stores and early neurologic deterioration in acute cerebral infarction Neurology, April 25, 2000; 54(8): 1568 - 1574. [Abstract] [Full Text] [PDF]

				H. Kawai, H. Nakai, M. Suga, S. Yuki, T. Watanabe, and K.-I. Saito Effects of a Novel Free Radical Scavenger, MCI-186, on Ischemic Brain Damage in the Rat Distal Middle Cerebral Artery Occlusion Model J. Pharmacol. Exp. Ther., May 1, 1997; 281(2): 921 - 927. [Abstract] [Full Text]

				I. Buchkremer-Ratzmann, M. August, G. Hagemann, and O. W. Witte Electrophysiological Transcortical Diaschisis After Cortical Photothrombosis in Rat Brain Stroke, June 1, 1996; 27(6): 1105 - 1111. [Abstract] [Full Text]

				E. Kozniewska, T. P. L. Roberts, M. Tsuura, J. Mintorovitch, M. E. Moseley, and J. Kucharczyk NG-Nitro-L-Arginine Delays the Development of Brain Injury During Focal Ischemia in Rats Stroke, February 1, 1995; 26(2): 282 - 289. [Abstract] [Full Text]