This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Takeshima, R. Articles by Traystman, R. J. Search for Related Content PubMed PubMed Citation Articles by Takeshima, R. Articles by Traystman, R. J.

Stroke, Vol 23, 247-252, Copyright © 1992 by American Heart Association

ARTICLES

Monoclonal leukocyte antibody does not decrease the injury of transient focal cerebral ischemia in cats

R Takeshima, JR Kirsch, RC Koehler, AW Gomoll and RJ Traystman
Department of Anesthesiology and Critical Care Medicine, Johns Hopkins Medical Institutions, Baltimore, Md.

BACKGROUND AND PURPOSE: We tested the hypothesis that inhibition of leukocyte function by administration of monoclonal antibody 60.3 (MoAb 60.3) improves electrophysiological recovery and decreases injury volume following transient focal cerebral ischemia in cats. METHODS: Halothane-anesthetized cats underwent 90 minutes of left middle cerebral artery and bilateral common carotid artery occlusion followed by 180 minutes of reperfusion. Cats were assigned to receive either 2 mg/kg MoAb 60.3 (n = 8) directed at the CDw18 leukocyte antigen complex or an equal volume of diluent (sterile saline; n = 10) at 45 minutes of ischemia in a blinded fashion. RESULTS: Blood flow to the left temporoparietal cortex decreased to less than 5 ml/min/100 g with ischemia, but was minimally affected on the right side. Postischemic hyperemia occurred in the left caudate nucleus, whereas blood flow in other brain regions returned to control. No region demonstrated delayed hypoperfusion, and there were no differences between groups. Somatosensory evoked potential recorded over the left cortex was ablated during ischemia and recovered to less than 10% of baseline amplitude at 180 minutes of reperfusion in both groups. Left hemispheric injury volume, as assessed by 2,3,5-triphenyltetrazolium chloride staining, was not affected by drug treatment (mean +/- SE values: MoAb 60.3, 37 +/- 5%; placebo, 38 +/- 7% of hemisphere). CONCLUSIONS: Inhibition of leukocyte function with MoAb 60.3 does not afford protection from severe focal ischemia and reperfusion in cats.

This article has been cited by other articles:

				S.-H. Ahmed, Y. Y. He, A. Nassief, J. Xu, X. M. Xu, C. Y. Hsu, and F. M. Faraci Effects of Lipopolysaccharide Priming on Acute Ischemic Brain Injury Editorial Comment Stroke, January 1, 2000; 31(1): 193 - 199. [Abstract] [Full Text] [PDF]

				C. J. Prestigiacomo, S. C. Kim, E. S. Connolly Jr, H. Liao, S.-F. Yan, D. J. Pinsky, and G. Feuerstein CD18-Mediated Neutrophil Recruitment Contributes to the Pathogenesis of Reperfused but Not Nonreperfused Stroke • Editorial Comment Stroke, May 1, 1999; 30(5): 1110 - 1117. [Abstract] [Full Text] [PDF]

				S. G. Soriano, A. Coxon, Y. F. Wang, M. P. Frosch, S. A. Lipton, P. R. Hickey, T. N. Mayadas, and P. H. Chan Mice Deficient in Mac-1 (CD11b/CD18) Are Less Susceptible to Cerebral Ischemia/Reperfusion Injury • Editorial Comment Stroke, January 1, 1999; 30(1): 134 - 139. [Abstract] [Full Text] [PDF]

				L. Pantoni, C. Sarti, and D. Inzitari Cytokines and Cell Adhesion Molecules in Cerebral Ischemia : Experimental Bases and Therapeutic Perspectives Arterioscler. Thromb. Vasc. Biol., April 1, 1998; 18(4): 503 - 513. [Abstract] [Full Text] [PDF]

				A. Bethel, J. R. Kirsch, R. C. Koehler, S. P. Finklestein, and R. J. Traystman Intravenous Basic Fibroblast Growth Factor Decreases Brain Injury Resulting From Focal Ischemia in Cats Stroke, March 1, 1997; 28(3): 609 - 616. [Abstract] [Full Text]

				H. Takahashi, J. R. Kirsch, K. Hashimoto, E. D. London, R. C. Koehler, and R. J. Traystman PPBP [4-Phenyl-1-(4-phenylbutyl) Piperidine], a Potent {varsigma}-Receptor Ligand, Decreases Brain Injury After Transient Focal Ischemia in Cats Stroke, September 1, 1995; 26(9): 1676 - 1682. [Abstract] [Full Text]