Stroke, Vol 23, 712-718, Copyright © 1992 by American Heart Association
E Mori, GJ del Zoppo, JD Chambers, BR Copeland and KE Arfors
BACKGROUND AND PURPOSE: While polymorphonuclear leukocytes may contribute
to the "no-reflow" phenomenon after focal cardiac and skeletal muscle
ischemia/reperfusion, their contribution to acute focal cerebral ischemia
is unresolved. We have examined the role of polymorphonuclear leukocytes in
microvascular perfusion defects after focal cerebral ischemia/reperfusion
in a baboon model of reversible middle cerebral artery occlusion with the
anti-CD18 monoclonal antibody IB4, which inhibits neutrophil adherence to
endothelium. METHODS: Microvascular patency in the basal ganglia after
3-hour middle cerebral artery occlusion and 1-hour reperfusion (by india
ink tracer perfusion) was quantified by computerized video imaging. Animals
were randomized to receive intravenous IB4 infusion 15 minutes before
reperfusion (n = 7) or to receive no treatment (n = 6). Binding of IB4 to
baboon leukocytes was maximal within 5 minutes of infusion. RESULTS: In the
untreated group, a significant reduction in patency was observed in
microvessels less than 30 microns diameter: mean percent reflow was 51% in
the capillary diameter class (4.0-7.5 microns) and 39% in the precapillary
arteriole and postcapillary venule diameter class (7.5-30 microns).
Infusion of IB4 before middle cerebral artery reperfusion increased reflow
in microvessels of all size classes, most significantly in those 7.5-30
microns (p = 0.049) and 30-50 microns (p = 0.034) in diameter. CONCLUSIONS:
These results suggest that CD18- mediated polymorphonuclear
leukocyte-endothelium adherence contributes to no-reflow predominantly in
noncapillary microvessels and at least partially to that in capillaries.
ARTICLES
Inhibition of polymorphonuclear leukocyte adherence suppresses no- reflow after focal cerebral ischemia in baboons
Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, Calif. 92037.
This article has been cited by other articles:
 |
|
 |
|
  B.J. Evans, D.O. Haskard, J.R. Finch, I.R. Hambleton, R.C. Landis, and K.M. Taylor The inflammatory effect of cardiopulmonary bypass on leukocyte extravasation in vivo. J. Thorac. Cardiovasc. Surg., May 1, 2008; 135(5): 999 - 1006. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. J. del Zoppo and R. Milner Integrin-Matrix Interactions in the Cerebral Microvasculature Arterioscler. Thromb. Vasc. Biol., September 1, 2006; 26(9): 1966 - 1975. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C. Harmon, K. G. Ghori, N. P. Eustace, S. J. F. O'Callaghan, A. P. O'Donnell, and G. D. Shorten Aprotinin decreases the incidence of cognitive deficit following CABG and cardiopulmonary bypass: a pilot randomized controlled study: [L'aprotinine reduit l'incidence de deficit cognitif a la suite d'un PAC et de la circulation extracorporelle : une etude pilote randomisee et controlee] Can J Anesth, December 1, 2004; 51(10): 1002 - 1009. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Harmon, E. Coleman, C. Marshall, W. Lan, and G. Shorten The Effect of Clomethiazole on Plasma Concentrations of Interleukin-6, -8, -1{beta}, Tumor Necrosis Factor-{alpha}, and Neutrophil Adhesion Molecule Expression During Experimental Extracorporeal Circulation Anesth. Analg., July 1, 2003; 97(1): 13 - 18. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Nishijima, J. Kiryu, A. Tsujikawa, M. Honjo, A. Nonaka, K. Yamashiro, H. Kamizuru, Y. Ieki, H. Tanihara, Y. Honda, et al. Inhibitory Effects of Antithrombin III on Interactions between Blood Cells and Endothelial Cells during Retinal Ischemia-Reperfusion Injury Invest. Ophthalmol. Vis. Sci., January 1, 2003; 44(1): 332 - 341. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.J.M. Frijns and L.J. Kappelle Inflammatory Cell Adhesion Molecules in Ischemic Cerebrovascular Disease Stroke, August 1, 2002; 33(8): 2115 - 2122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Kent, V. M. Soukup, and R. H. Fabian Heterogeneity Affecting Outcome From Acute Stroke Therapy: Making Reperfusion Worse Stroke, October 1, 2001; 32(10): 2318 - 2327. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. J. del Zoppo, K. J. Becker, and J. M. Hallenbeck Inflammation After Stroke: Is It Harmful? Arch Neurol, April 1, 2001; 58(4): 669 - 672. [Full Text] [PDF]
|
|
|
|
 |
|
 K. Miyakoshi, H. Ishimoto, O. Nishimura, S. Tanigaki, M. Tanaka, T. Miyazaki, M. Natori, and Y. Yoshimura Role of leukocytes in uterine hypoperfusion and fetal growth retardation induced by ischemia-reperfusion Am J Physiol Heart Circ Physiol, March 1, 2001; 280(3): H1215 - H1221. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Becker, D. Kindrick, J. Relton, J. Harlan, R. Winn, and M. A. Yenari Antibody to the {{alpha}}4 Integrin Decreases Infarct Size in Transient Focal Cerebral Ischemia in Rats Editorial Comment Stroke, January 1, 2001; 32(1): 206 - 211. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Huang, T. F. Choudhri, C. J. Winfree, R. A. McTaggart, S. Kiss, J. Mocco, L. J. Kim, T. S. Protopsaltis, Y. Zhang, D. J. Pinsky, et al. Postischemic Cerebrovascular E-Selectin Expression Mediates Tissue Injury in Murine Stroke Editorial Comment Stroke, December 1, 2000; 31(12): 3047 - 3053. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Abumiya, R. Fitridge, C. Mazur, B. R. Copeland, J. A. Koziol, J. F. Tschopp, M. D. Pierschbacher, G. J. del Zoppo, and P. D. Hurn Integrin {alpha}IIb{beta}3 Inhibitor Preserves Microvascular Patency in Experimental Acute Focal Cerebral Ischemia Editorial Comment Stroke, June 1, 2000; 31(6): 1402 - 1410. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ishikawa, E. Sekizuka, S. Sato, N. Yamaguchi, J. Inamasu, H. Bertalanffy, T. Kawase, and C. Iadecola Effects of Moderate Hypothermia on Leukocyte- Endothelium Interaction in the Rat Pial Microvasculature After Transient Middle Cerebral Artery Occlusion • Editorial Comment Stroke, August 1, 1999; 30(8): 1679 - 1686. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Zhang, Z. G. Zhang, M. Chopp, and J. A. Zivin Thrombolysis With Tissue Plasminogen Activator Alters Adhesion Molecule Expression in the Ischemic Rat Brain • Editorial Comment Stroke, March 1, 1999; 30(3): 624 - 629. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. G. Soriano, A. Coxon, Y. F. Wang, M. P. Frosch, S. A. Lipton, P. R. Hickey, T. N. Mayadas, and P. H. Chan Mice Deficient in Mac-1 (CD11b/CD18) Are Less Susceptible to Cerebral Ischemia/Reperfusion Injury • Editorial Comment Stroke, January 1, 1999; 30(1): 134 - 139. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bitsch, W. Klene, L. Murtada, H. Prange, and P. Rieckmann A Longitudinal Prospective Study of Soluble Adhesion Molecules in Acute Stroke Stroke, October 1, 1998; 29(10): 2129 - 2135. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Maier, K. vB. Ahern, M. L. Cheng, J. E. Lee, M. A. Yenari, G. K. Steinberg, and J. R. Kirsch Optimal Depth and Duration of Mild Hypothermia in a Focal Model of Transient Cerebral Ischemia : Effects on Neurologic Outcome, Infarct Size, Apoptosis, and Inflammation • Editorial Comment: Effects on Neurologic Outcome, Infarct Size, Apoptosis, and Inflammation Stroke, October 1, 1998; 29(10): 2171 - 2180. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bavbek, R. Polin, A.-L. Kwan, A. S. Arthur, N. F. Kassell, K. S. Lee, and R. L. Macdonald Monoclonal Antibodies Against ICAM-1 and CD18 Attenuate Cerebral Vasospasm After Experimental Subarachnoid Hemorrhage in Rabbits • Editorial Comment Stroke, September 1, 1998; 29(9): 1930 - 1936. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kanwar, M. J. Hickey, and P. Kubes Postischemic inflammation: a role for mast cells in intestine but not in skeletal muscle Am J Physiol Gastrointest Liver Physiol, August 1, 1998; 275(2): G212 - G218. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. J DEL ZOPPO, R. VON KUMMER, and G. F HAMANN Ischaemic damage of brain microvessels: inherent risks for thrombolytic treatment in stroke J. Neurol. Neurosurg. Psychiatry, July 1, 1998; 65(1): 1 - 9. [Full Text]
|
|
|
|
 |
|
 J. R. Bethea, M. Castro, R. W. Keane, T. T. Lee, W. D. Dietrich, and R. P. Yezierski Traumatic Spinal Cord Injury Induces Nuclear Factor-kappa B Activation J. Neurosci., May 1, 1998; 18(9): 3251 - 3260. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. Solenski, A.-L. Kwan, H. Yanamoto, J. P. Bennett, N. F. Kassell, and K. S. Lee Differential Hydroxylation of Salicylate in Core and Penumbra Regions During Focal Reversible Cerebral Ischemia Stroke, December 1, 1997; 28(12): 2545 - 2552. [Abstract] [Full Text]
|
|
|
|
|
|
C.J.M. Frijns, L.J. Kappelle, J. v. Gijn, H.K. Nieuwenhuis, J.J. Sixma, and R. Fijnheer Soluble Adhesion Molecules Reflect Endothelial Cell Activation in Ischemic Stroke and in Carotid Atherosclerosis Stroke, November 1, 1997; 28(11): 2214 - 2218. [Abstract] [Full Text]
|
|
|
|
 |
|
 L. Ostrovsky, R. C. Woodman, D. Payne, D. Teoh, and P. Kubes Antithrombin III Prevents and Rapidly Reverses Leukocyte Recruitment in Ischemia/Reperfusion Circulation, October 7, 1997; 96(7): 2302 - 2310. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. J. Becker, R. M. McCarron, C. Ruetzler, O. Laban, E. Sternberg, K. C. Flanders, and J. M. Hallenbeck Immunologic tolerance to myelin basic protein decreases stroke size after transient focal cerebral ischemia PNAS, September 30, 1997; 94(20): 10873 - 10878. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. S. Connolly Jr, C. J. Winfree, C. J. Prestigiacomo, S. C. Kim, T. F. Choudhri, B. L. Hoh, Y. Naka, R. A. Solomon, and D. J. Pinsky Exacerbation of Cerebral Injury in Mice That Express the P-Selectin Gene : Identification of P-Selectin Blockade as a New Target for the Treatment of Stroke Circ. Res., September 19, 1997; 81(3): 304 - 310. [Abstract] [Full Text]
|
|
|
|
|
|
P. J. Lindsberg, O. Carpen, A. Paetau, M.-L. Karjalainen-Lindsberg, and M. Kaste Endothelial ICAM-1 Expression Associated With Inflammatory Cell Response in Human Ischemic Stroke Circulation, September 1, 1996; 94(5): 939 - 945. [Abstract] [Full Text]
|
|
|
|
|
|
H.-P. Haring, E. L. Berg, N. Tsurushita, M. Tagaya, G. J. del Zoppo, and M. J Eppihimer E-Selectin Appears in Nonischemic Tissue During Experimental Focal Cerebral Ischemia Stroke, August 1, 1996; 27(8): 1386 - 1392. [Abstract] [Full Text]
|
|
|
|
|
|
K. H. Ryu, B. J. Hindman, D. K. Reasoner, and F. Dexter Heparin Reduces Neurological Impairment After Cerebral Arterial Air Embolism in the Rabbit Stroke, February 1, 1996; 27(2): 303 - 310. [Abstract] [Full Text]
|
|
|
|
 |
|
 B. K. Siesjo, Q. Zhao, K. Pahlmark, P. Siesjo, K.-i. Katsura, and J. Folbergrova Glutamate, Calcium, and Free Radicals as Mediators of Ischemic Brain Damage Ann. Thorac. Surg., May 1, 1995; 59(5): 1316 - 1320. [Abstract] [Full Text]
|
|
|
|
|
|
J. H. Garcia, K.-F. Liu, and K.-L. Ho Neuronal Necrosis After Middle Cerebral Artery Occlusion in Wistar Rats Progresses at Different Time Intervals in the Caudoputamen and the Cortex Stroke, April 1, 1995; 26(4): 636 - 643. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. Edwards Protection against hypoxic-ischaemic cerebral injury in the developing brain Perfusion, January 1, 1993; 8(1): 97 - 100. [PDF]
|
|