This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wester, P. Articles by Globus, M. Y. Search for Related Content PubMed PubMed Citation Articles by Wester, P. Articles by Globus, M. Y.

Stroke, Vol 23, 870-875, Copyright © 1992 by American Heart Association

ARTICLES

Serotonin release into plasma during common carotid artery thrombosis in rats

P Wester, WD Dietrich, R Prado, BD Watson and MY Globus
Department of Neurology, University of Miami School of Medicine, Fla. 33101.

BACKGROUND AND PURPOSE: We have tested the hypothesis that platelet- derived serotonin is released into the bloodstream during cerebrovascular thrombosis. METHODS: Nonocclusive common carotid artery thrombosis was produced photochemically in 22 anesthetized adult male Wistar rats using the photosensitizing dye rose bengal and irradiation with an argon-pumped dye laser. Plasma serotonin levels were recorded before, during, and after photothrombosis by intra-arterial in vivo microdialysis with the probe placed distal to the site of thrombosis. RESULTS: During and immediately after the common carotid artery thrombosis, serotonin levels increased significantly to a peak value of 781 nmol/l (p less than 0.001 by analysis of variance), representing a 15-fold increase compared with baseline levels. The increased serotonin levels gradually decreased but remained significantly elevated for 90 minutes. Ultrastructural analysis of the carotid thrombi identified a dense mass of aggregated platelets at various stages of degranulation. CONCLUSIONS: These results are the first to demonstrate directly that serotonin accumulation occurs in plasma during and after the acute phase of common carotid artery thrombosis. Increased plasma serotonin levels may play a major role in the cerebral blood flow and blood-brain barrier abnormalities previously documented in this model of large- vessel thrombotic stroke.

This article has been cited by other articles:

				H. Yao, H. Sugimori, K. Fukuda, J. Takada, H. Ooboshi, T. Kitazono, S. Ibayashi, and M. Iida Photothrombotic Middle Cerebral Artery Occlusion and Reperfusion Laser System in Spontaneously Hypertensive Rats Stroke, November 1, 2003; 34(11): 2716 - 2721. [Abstract] [Full Text] [PDF]

				W. D. Dietrich, G. Danton, A. C. Hopkins, R. Prado, and W. Pearce Thromboembolic Events Predispose the Brain to Widespread Cerebral Infarction After Delayed Transient Global Ischemia in Rats • Editorial Comment Stroke, April 1, 1999; 30(4): 855 - 862. [Abstract] [Full Text] [PDF]

				H. Yao, S. Ibayashi, H. Sugimori, K. Fujii, and M. Fujishima Simplified Model of Krypton LaserInduced Thrombotic Distal Middle Cerebral Artery Occlusion in Spontaneously Hypertensive Rats Stroke, February 1, 1996; 27(2): 333 - 336. [Abstract] [Full Text]

				N. E. Alexis, W. D. Dietrich, E. J. Green, R. Prado, and B. D. Watson Nonocclusive Common Carotid Artery Thrombosis in the Rat Results in Reversible Sensorimotor and Cognitive Behavioral Deficits Stroke, December 1, 1995; 26(12): 2338 - 2346. [Abstract] [Full Text]

				P. Wester, B. D. Watson, R. Prado, and W. D. Dietrich A Photothrombotic `Ring' Model of Rat Stroke-in-Evolution Displaying Putative Penumbral Inversion Stroke, March 1, 1995; 26(3): 444 - 450. [Abstract] [Full Text]

				T. Iwamoto, H. Kubo, and M. Takasaki Platelet Activation in the Cerebral Circulation in Different Subtypes of Ischemic Stroke and Binswanger's Disease Stroke, January 1, 1995; 26(1): 52 - 56. [Abstract] [Full Text]

				W. D. Dietrich, L. P. Miller, R. Prado, S. Dewanjee, N. Alexis, M. K. Dewanjee, and H. Gruber Acadesine Reduces Indium-Labeled Platelet Deposition After Photothrombosis of the Common Carotid Artery in Rats Stroke, January 1, 1995; 26(1): 111 - 116. [Abstract] [Full Text]