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Stroke, Vol 24, 1576-1583, Copyright © 1993 by American Heart Association

ARTICLES

Fructose-1,6-bisphosphate reduces infarct volume after reversible middle cerebral artery occlusion in rats

JW Kuluz, GA Gregory, Y Han, WD Dietrich and CL Schleien
Department of Pediatrics, University of Miami, School of Medicine, FL 33101.

BACKGROUND AND PURPOSE: We tested the hypothesis that fructose-1,6- bisphosphate, when administered 10 minutes before the end of 2 hours of reversible middle cerebral artery occlusion, reduces ischemia- reperfusion injury and infarct volume measured after a 3-day survival period in rats. METHODS: After 1 hour and 50 minutes of middle cerebral artery occlusion by the intraluminal suture method, fructose-1,6- bisphosphate, 500 mg/kg in group 1 and 350 mg/kg in group 2 (or an equivalent volume of 1.8% saline as placebo in each group), was given intravenously for a period of 15 minutes to fasted adult Sprague-Dawley rats. After 2 hours of ischemia, the suture was withdrawn and the rats allowed to survive for 3 days. The areas of infarction in 10 hematoxylin-eosin-stained coronal sections of the brain were measured and used to calculate infarct volume. RESULTS: In group 1, fructose-1,6- bisphosphate decreased total cerebral hemispheric infarct volume by 43% (from 199.6 +/- 11.2 to 114.2 +/- 35.8 mm3, P < .04; mean +/- SEM). Cerebral cortical and subcortical infarct volumes were decreased by 46% (from 137.3 +/- 7.5 to 74.1 +/- 28.6 mm3, P < .04) and 36% (from 62.3 +/- 5.1 to 40.0 +/- 8.3 mm3, P < .04), respectively. In group 2, fructose-1,6-bisphosphate had no effect on infarct volume in rats that developed mild intraischemic hyperthermia, but in rats kept normothermic during ischemia, fructose-1,6-bisphosphate reduced subcortical infarct volume from 53.7 +/- 8.1 to 18.4 +/- 8.0 mm3 (P < .03). CONCLUSIONS: Fructose-1,6-bisphosphate improves functional neurological outcome and reduces infarct volume after reversible middle cerebral artery occlusion in rats.

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				F. Li, T. Omae, M. Fisher, W. D. Dietrich, and J. W. Kuluz Spontaneous Hyperthermia and its Mechanism in the Intraluminal Suture Middle Cerebral Artery Occlusion Model of Rats • Editorial Comment Stroke, November 1, 1999; 30(11): 2464 - 2471. [Abstract] [Full Text] [PDF]