Stroke, Vol 24, 294-297, Copyright © 1993 by American Heart Association
RJ Laing, J Jakubowski and RW Laing
BACKGROUND AND PURPOSE: Our purpose was to assess the effectiveness of
middle cerebral artery occlusion in producing acute focal ischemia in the
rat by the use of Koizumi's and Longa's methods, in which occlusion is
achieved by passing a nylon thread into the internal carotid artery.
METHODS: Cerebral blood flow was measured by using the hydrogen clearance
method, and the brains were examined histologically to assess ischemic
damage. RESULTS: By Koizumi's method profound reduction in cerebral blood
flow was achieved in 28 of 30 rats (93%). The mean cerebral blood flow in
the middle cerebral artery territory was 10.7 (95% confidence interval,
9.9-11.5) ml/100 g per minute. By Longa's method reduction in cerebral
blood flow was achieved in only 29 of 52 rats (56%), and in these animals
mean cerebral blood flow was 33 (95% confidence interval, 28.3-33.7) ml/100
g per minute (p < 0.001 compared with Koizumi's method). Cerebral blood
flow was reduced to < 16 ml/100 g per minute in only seven animals
(24%). CONCLUSIONS: By Koizumi's method the depth of ischemia is more
profound, occlusion is achieved in a much higher proportion of cases, and
the incidence of perforation of the intracranial internal carotid is much
less frequent than by Longa's method.
ARTICLES
Middle cerebral artery occlusion without craniectomy in rats. Which method works best?
Department of Neurosurgery, Royal Hallamshire Hospital, Sheffield, UK.
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