Stroke, Vol 24, 415-420, Copyright © 1993 by American Heart Association
K Miyake, S Takeo and H Kaijihara
BACKGROUND AND PURPOSE: An experimental model that induces sustained
ischemia and infarction may provide useful information relevant to
prevention of the development of ischemic brain disease. The purpose of the
present study was to elucidate the pathophysiological consequences of
cerebral blood flow under sustained cerebral ischemia or oligemia and
infarction in rats after microsphere embolism. METHODS: We injected 900
microspheres (48 microns in diameter) into the right internal carotid
artery of 146 rats and determined the time course of changes in blood flow
of the cerebral cortex, striatum, and hippocampus of both hemispheres by
the hydrogen clearance method for a period of 28 days after the operation.
Infarct area was determined by triphenyltetrazolium chloride staining and
hematoxylin and eosin staining methods. RESULTS: Cortical and striatal
blood flow of the right hemisphere of microsphere-injected rats was
significantly decreased after the embolism, and this was sustained
throughout the experiment. Hippocampal blood flow of the
microsphere-injected hemisphere was also decreased on days 1 and 3 but
tended to return toward control levels thereafter. In the left hemisphere,
reduction in regional blood flow was detected in the cortex and hippocampus
on day 1 and the striatum on day 3. A triphenyltetrazolium
chloride-unstained area had developed by day 3 after the embolism. The
extent of the area was similar to that on days 7 and 28. Microscopic
examination revealed degenerative areas scattered mainly in the
parietotemporal cortex, corpus callosum, hippocampus, thalamus, and
lenticular nucleus of the embolized hemisphere, demonstrating the induction
of widespread necrosis after embolism. CONCLUSIONS: Microsphere embolism
resulted in a sustained decrease in regional blood flow and production of
cerebral infarction in the brain regions of the microsphere-injected
hemisphere.
ARTICLES
Sustained decrease in brain regional blood flow after microsphere embolism in rats
Department of Pharmacology, Tokyo College of Pharmacy, Japan.
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