This Article Full Text Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Itoh, T. Articles by Kamada, T. Search for Related Content PubMed PubMed Citation Articles by Itoh, T. Articles by Kamada, T.

(Stroke. 1995;26:2353-2357.)
© 1995 American Heart Association, Inc.

Articles

Perfusion Imaging of the Brain by B-Mode Ultrasonography

An Experimental Study in Rabbits

Taiji Itoh, MD; Masayasu Matsumoto, MD, PhD; Ryoko Uchimoto, BS; Natsuko Takahashi, BS; Kazuo Niwa, BS; Nobuo Handa, MD, PhD; Yoshitane Tsukamoto, MD; Tomoaki Miyazawa, PhD Takenobu Kamada, MD, PhD

From the First Department of Internal Medicine (T.I., M.M., N.H., T.K.) and the Department of Pathology (Y.T.), Osaka University Medical School; and the Research Department of Nihon Schering, K.K. (R.U., N.T., K.N., T.M.), Osaka, Japan.

Correspondence to Taiji Itoh, MD, First Department of Internal Medicine, Osaka University Medical School, Yamadaoka, 2-2, Suita, Osaka 565, Japan.

Background and Purpose The purpose of this study was to assess the ultrasonic imaging of normal rabbit brain by using echocontrast enhancer to identify the brain tissue perfusion.

Methods A solution of SH/TA-508 (containing microbubbles of mean size 2 to 4 µm; n=4) was used for contrast enhancement of rabbit brain tissue and was administered through the left internal carotid artery. Contrast enhancement in the ipsilateral parietal area was examined by placing a 7.5-MHz transducer on the dura after a craniectomy. Throughout the experiment, we measured parameters including arterial blood pressure, arterial blood gases, and serum glucose and hematocrit levels. We investigated whether the administration of an echocontrast enhancer would be safe for the brain tissue (n=4).

Results After injection of 100 mg/mL SH/TA-508, contrast enhancement was clearly observed within approximately 1 second in the left hemisphere (especially in the left cerebral cortex) in all animals and disappeared within 3 seconds after the end of injection. All physiological parameters remained within the normal range throughout the experiment. No focal abnormalities were observed on hematoxylin-eosin staining of the brain tissue.

Conclusions Contrast enhancement was observed in the ipsilateral hemisphere with the administration of SH/TA-508. Contrast enhancement on B-mode brain imaging would be useful for real-time identification of brain tissue perfusion and should become a promising method for bedside clinical assessment of brain tissue perfusion.

Key Words: contrast media • perfusion • ultrasonics • rabbits

This article has been cited by other articles:

				T. Shiogai, N. Takayasu, T. Mizuno, M. Nakagawa, and H. Furuhata Comparison of Transcranial Brain Tissue Perfusion Images Between Ultraharmonic, Second Harmonic, and Power Harmonic Imaging Stroke, March 1, 2004; 35(3): 687 - 693. [Abstract] [Full Text] [PDF]

				J. U. Harrer and C. Klotzsch Second Harmonic Imaging of the Human Brain: The Practicability of Coronal Insonation Planes and Alternative Perfusion Parameters Stroke, June 1, 2002; 33(6): 1530 - 1535. [Abstract] [Full Text] [PDF]

				G. Deklunder Role of ultrasound and contrast-enhanced ultrasound in patients with cerebrovascular disease Eur. Heart J. Suppl., March 1, 2002; 4(suppl_C): C51 - C55. [Abstract] [PDF]

				C. Pohl, K. Tiemann, T. Schlosser, and H. Becher Stimulated Acoustic Emission Detected by Transcranial Color Doppler Ultrasound : A Contrast-Specific Phenomenon Useful for the Detection of Cerebral Tissue Perfusion Stroke, July 1, 2000; 31(7): 1661 - 1666. [Abstract] [Full Text] [PDF]

				T. Gerriets, G. Seidel, I. Fiss, B. Modrau, and M. Kaps Contrast-enhanced transcranial color-coded duplex sonography: Efficiency and validity Neurology, April 1, 1999; 52(6): 1133 - 1133. [Abstract] [Full Text] [PDF]