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(Stroke. 1995;26:1067-1071.)
© 1995 American Heart Association, Inc.

Articles

Alterations of Brain Levels of Phosphoinositidase-C–Linked Gq/G11 Proteins and Motor Function in Rats After Cardiac Arrest

Shyhwen P. Jaw, PhD; Duan D. Su, PharmD; Rae R. Matsumoto, PhD Daniel D. Truong, MD

From the Parkinson and Movement Disorders Laboratory, Department of Neurology, University of California, Irvine.

Background and Purpose Phosphoinositidase-C–linked G_q and G₁₁ proteins have only recently been characterized. Second messenger systems are known to be affected by hypoxia-ischemia. However, the effects of hypoxia-ischemia on the brain levels of G_q and G₁₁ proteins are not known. Therefore, in the present studies, the effects of hypoxia-ischemia on G_q and G₁₁ proteins in rats were investigated with quantitative immunoblot analysis.

Methods Cardiac arrest was induced in male Sprague-Dawley rats by an intracardial injection of KCl. Resuscitation began 10 minutes afterwards. At various time points after resuscitation, animals were killed and the cerebral cortex, striatum, and cerebellum were dissected. Levels of G_q and G₁₁ proteins were investigated by quantitative immunoblot analysis.

Results At 1, 2, 4, and 6 hours after resuscitation, G_q and G₁₁ protein levels remained unaltered. However, a significant reduction of these proteins was seen in the cerebral cortex and cerebellum of rats 3 and 14 days after cardiac arrest, with partial recovery by an average of 60 days. In contrast, no significant change was detected in the striatum.

Conclusions These observations indicate that phosphoinositidase-C–linked signal transduction pathways may be attenuated after hypoxic-ischemic insults to the brain, and that this phenomenon, together with many other factors, may contribute to the expression of motor dysfunction in rats after cardiac arrest.

Key Words: hypoxia • ischemia • proteins • rats • myoclonus