This Article Full Text Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lin, B. Articles by Ginsberg, M. D. Search for Related Content PubMed PubMed Citation Articles by Lin, B. Articles by Ginsberg, M. D.

(Stroke. 1995;26:1634-1638.)
© 1995 American Heart Association, Inc.

Articles

Brain Temperature Modulations During Global Ischemia Fail to Influence Extracellular Lactate Levels in Rats

Baowan Lin, MD; Raul Busto, BS; Mordecai Y.-T. Globus, MD; Elena Martinez, MS Myron D. Ginsberg, MD

From the Cerebral Vascular Disease Research Center, Department of Neurology, University of Miami (Fla) School of Medicine.

Background and Purpose While brain tissue lactate increases during cerebral ischemia and is known to be important in the pathogenesis of ischemic brain injury, patterns of extracellular lactate accumulation have been less well characterized, and the influence of brain temperature has not been previously investigated. Mild brain temperature modulations are known to affect the outcome of ischemia dramatically. This study examined changes of extracellular lactate during and after global cerebral ischemia, in which intraischemic brain temperature was held at either 30°C, 37°C, or 39°C.

Methods Halothane-anesthetized fasted male Wistar rats underwent 20 minutes of global cerebral ischemia produced by bilateral carotid artery occlusions plus systemic hypotension (40 to 50 mm Hg). Rectal temperature was maintained at 37°C throughout, and intraischemic brain temperature was held at either 30°C (n=6), 37°C (n=5), or 39°C (n=5). Before and after the ischemic insult, brain temperature was maintained at 37°C in all groups. A microdialysis cannula was implanted in the right dorsolateral striatum and perfused with Ringer's solution. Dialysate samples were collected at 10-minute intervals before, during, and after ischemia and were analyzed for lactate by enzymatic-fluorometric techniques.

Results In all groups, extracellular lactate rose during ischemia and peaked at 10 to 30 minutes of recirculation. Maximal extracellular lactate elevations were sevenfold, eightfold, and eightfold above control in the 30°C, 37°C, 39°C groups, respectively. Significant elevations with respect to control were observed in all groups at 10 to 30 minutes of recirculation. In the 30°C group, these elevations above control were also significant at the 10- and 20-minute ischemic time points (P=.001). At 30 minutes of recirculation, however, lactate levels were lower in the 30°C rats than in the other groups.

Conclusions These data provide evidence that extracellular lactate accumulation is not a crucial determinant of ischemic brain injury. Our results suggest that the increased lactate release during ischemia and the accelerated clearance of lactate during recirculation might contribute in part to the neuroprotection of intraischemic hypothermia.

Key Words: acidosis • hyperthermia • hypothermia • selective vulnerability • rats

This article has been cited by other articles:

				A. Martin, S. Rojas, A. Chamorro, C. Falcon, N. Bargallo, and A. M. Planas Why Does Acute Hyperglycemia Worsen the Outcome of Transient Focal Cerebral Ischemia?: Role of Corticosteroids, Inflammation, and Protein O-Glycosylation Stroke, May 1, 2006; 37(5): 1288 - 1295. [Abstract] [Full Text] [PDF]

				A. McCormick, C. Suguihara, J. Huang, C. Devia, D. Hehre, J. H. Bruce, and E. Bancalari Depressed ventilatory response to hypoxia in hypothermic newborn piglets: role of glutamate J Appl Physiol, March 1, 1998; 84(3): 830 - 836. [Abstract] [Full Text] [PDF]

				N. Kawai, R. F. Keep, A. L. Betz, and W. D. Dietrich Hyperglycemia and the Vascular Effects of Cerebral Ischemia Stroke, January 1, 1997; 28(1): 149 - 154. [Abstract] [Full Text]