(Stroke. 1996;27:1616-1623.)
© 1996 American Heart Association, Inc.
Articles |
Middle Cerebral Artery Occlusion in the Rat by Intraluminal Suture
Neurological and Pathological Evaluation of an Improved Model
the Cerebral Vascular Disease Research Center, Department of Neurology, University of Miami School of Medicine, Miami, Fla.
Background and Purpose The purpose of the present study was to evaluate a modified method of intraluminal suture occlusion of the middle cerebral artery (MCA) on the volume of brain infarction and on neurobehavioral function in rats subjected to a temporary focal ischemic insult.
Methods Male Sprague-Dawley rats were anesthetized with halothane and subjected to 60 minutes or 2 hours of temporary MCA occlusion (MCAo) by an intraluminal thread. In one group of rats, the suture was coated with poly-L-lysine, while in a second group, a conventional uncoated suture was used. Behavioral function was evaluated at 50 to 60 minutes after occlusion and during a 3-day period after MCAo. Three days after MCAo, brains were perfusion-fixed and infarct volumes were measured.
Results In rats with 60-minute MCAo, only 3 of 7 animals with uncoated sutures had infarcts, whereas in the group with poly-L-lysine–coated sutures, all rats (n=7) exhibited infarction (P=.009, Fisher's exact test). With 2 hours of MCAo, total infarct volume (corrected for brain edema) was significantly larger in rats with poly-L-lysine–coated sutures than in the group with uncoated sutures (mean±SEM, 122.1±4.8 versus 67.0±18.2 mm3, respectively; P=.03; n=4 in each group). In the 2-hour MCAo study, infarct volumes in the uncoated-suture group tended to be variable and inconsistent (coefficient of variation, 54%) compared with the group in which sutures were coated with poly-L-lysine, in which a highly consistent infarct was produced (coefficient of variation of infarct volume, 8%).
Conclusions Reversible MCAo in which a poly-L-lysine–coated intraluminal suture was used proved to be a reliable and effective modification of this technique, yielding consistently larger infarcts and greatly reduced interanimal variability.
Editorial Comment
Neurological and Pathological Evaluation of an Improved Model
Cerebrovascular Disease SectionDepartment of NeurologyWashington University School of MedicineSt. Louis, Mo
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