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(Stroke. 1997;28:2259-2265.)
© 1997 American Heart Association, Inc.

Articles

Neither L-Arginine nor L-NAME Affects Neurological Outcome After Global Ischemia in Cats

Jeffrey R. Kirsch, MD; Anish Bhardwaj, MD; Lee J. Martin, PhD; Daniel F. Hanley, MD; Richard J. Traystman, PhD

From the Departments of Anesthesiology and Critical Care Medicine and Neurology and Pathology, The Johns Hopkins Medical Institutions, Baltimore, Md.

Background and Purpose We attempted to determine whether N-nitro-L-arginine methyl ester (L-NAME) would improve neurological outcome and whether L-arginine (L-ARG) would worsen neurological outcome after transient global ischemia.

Methods Halothane-anesthetized cats (n=6 for each group) were treated with intravenous saline, L-NAME (5 mg/kg or 10 mg/kg), or L-arginine (300 mg/kg) 30 minutes before 10 minutes of ischemia (temporary ligation of the left subclavian and brachiocephalic arteries with hemorrhagic hypotension to 50 mm Hg). At 30 minutes of reperfusion, cats in the L-ARG group were administered an additional 300 mg/kg dose of intravenous L-arginine.

Results Time (mean±SE) to isoelectric electroencephalography was similar among groups (saline, 26±11 seconds; L-NAME–5, 15±4 seconds; L-NAME–10, 36±27 seconds; and L-ARG, 22±7 seconds). At 72 hours, reperfusion pathological injury was severe and neurological deficit score (mean, range) was similar among groups (saline, 38 [11 to 70]; L-NAME–5, 52 [40 to 73]; L-NAME–10, 47 [23 to 70]; and L-ARG, 40 [0 to 79]).

Conclusions Nitric oxide is not important in the mechanism of brain injury after global ischemia in cats.

Key Words: cerebral ischemia, global • neuroprotection • nitric oxide • neuronal death • cats

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