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(Stroke. 1999;30:867-872.)
© 1999 American Heart Association, Inc.

Original Contributions

Amelioration of Vasospasm After Subarachnoid Hemorrhage in Transgenic Mice Overexpressing CuZn–Superoxide Dismutase

Hideyuki Kamii, MD, PhD; Ichiro Kato, MD, PhD; Hiroyuki Kinouchi, MD, PhD; Pak H. Chan, PhD; Charles J. Epstein, MD; Atsushi Akabane, MD, PhD; Hiroshi Okamoto, MD, PhD Takashi Yoshimoto, MD

From the Departments of Neurosurgery (H.K., A.A., T.Y.) and Biochemistry (I.K., H.O.), Tohoku University School of Medicine, Sendai, Japan; the Department of Neurosurgery, Akita University School of Medicine, Akita, Japan (H.K); and the Departments of Neurosurgery (P.H.C), Neurology (P.H.C), and Pediatrics (C.J.E), University of California, San Francisco.

Correspondence to Hideyuki Kamii, MD, PhD, Department of Neurosurgery, Tohoku University School of Medicine, 1-1 Seiryomachi, Aoba-ku, Sendai 980-8574, Japan. E-mail kamii{at}nsg.med.tohoku.ac.jp

Background and Purpose—To clarify the effect of superoxide dismutase (SOD) on vasospasm after subarachnoid hemorrhage (SAH), we investigated sequential changes in arterial diameter after SAH in transgenic mice overexpressing CuZn-SOD (SOD-1).

Methods—SOD-transgenic mice and nontransgenic littermates (35 to 40 g) were subjected to SAH produced by endovascular perforation of left anterior cerebral artery. At 4 hours and 1, 3, 7, and 14 days after SAH, the mice were perfused with 10% formalin and consequently with a mixture of carbon black and 10% gelatin to cast all vessels. Vasospasm was evaluated by measuring the diameter of the left middle cerebral artery (MCA) with a microscope.

Results—In nontransgenic mice, the diameter of the MCA on day 3 after SAH (110.5±20.5 µm [mean±SD]; n=16) was significantly reduced compared with that without SAH (138.5±14.5 µm; n=12) (P<0.01). Moreover, on day 3 after SAH, the diameter of the MCA in SOD-transgenic mice (127.9±20.2 µm; n=20) was significantly larger than that in nontransgenic mice (110.5±20.5 µm; n=16) (P<0.05).

Conclusions—These results suggest that SOD is effective on the amelioration of vasospasm after SAH and that oxygen free radicals, particularly superoxide, play an important role in the pathogenesis of vasospasm after SAH.

Editorial Comment

Chung Y. Hsu, MD, PhD, Guest Editor

Department of Neurology, Cerebrovascular Disease Section, Washington University School of Medicine, St Louis, Missouri

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