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(Stroke. 2000;31:1945.)
© 2000 American Heart Association, Inc.

Original Contributions

White Matter Injury in Spinal Cord Ischemia

Protection by AMPA/Kainate Glutamate Receptor Antagonism

Georgios K. Kanellopoulos, MD; Xiao Ming Xu, MD, PhD; Chung Y. Hsu, MD, PhD; Xiaobin Lu, MD; Thoralf M. Sundt, MD Nicholas T. Kouchoukos, MD

From the Division of Cardiothoracic Surgery, Department of Surgery (G.K.K., T.M.S.), and Department of Neurology and the Center for the Study of Nervous System Injury (C.Y.H.), Washington University School of Medicine; the Department of Anatomy and Neurobiology, St Louis University School of Medicine (X.M.X., X.L.); and the Department of Surgery, Missouri Baptist Hospital (N.T.K.), St Louis.

Correspondence to Dr Chung Y. Hsu, Department of Neurology, Washington University School of Medicine, St Louis, MO 63110. E-mail hsuc{at}neuro.wustl.edu

Background and Purpose—Spinal cord ischemia is a serious complication of surgery of the aorta. NMDA receptor activation secondary to ischemia-induced release of glutamate is a major mechanism of neuronal death in gray matter. White matter injury after ischemia results in long-tract dysfunction and disability. The AMPA/kainate receptor mechanism has recently been implicated in white matter injury.

Methods—We studied the effects of AMPA/kainate receptor blockade on ischemic white matter injury in a rat model of spinal cord ischemia.

Results—Intrathecal administration of an AMPA/kainate antagonist, 6-nitro-7-sulfamoyl-(f)-quinoxaline-2,3-dione (NBQX), 1 hour before ischemia reduced locomotor deficit, based on the Basso-Beattie-Bresnahan scale (0=total paralysis; 21=normal) (sham: 21±0, n=3; saline: 3.7±4.5, n=7; NBQX: 12.7±7.0, n=7, P<0.05) 6 weeks after ischemia. Gray matter damage and neuronal loss in the ventral horn were evident after ischemia, but no difference was noted between the saline and NBQX groups. The extent of white matter injury was quantitatively assessed, based on axonal counts, and was significantly less in the NBQX as compared with the saline group in the ventral (sham: 1063±44/200x200 µm, n=3; saline: 556±104, n=7; NBQX: 883±103, n=7), ventrolateral (sham: 1060±135, n=3; saline: 411±66, n=7; NBQX: 676±122, n=7), and corticospinal tract (sham: 3391±219, n=3; saline: 318±23, n=7; NBQX: 588±103, n=7) in the white matter on day 42.

Conclusions—Results indicate severe white matter injury in the spinal cord after transient ischemia. NBQX, an AMPA/kainate receptor antagonist, reduced ischemia-induced white matter injury and improved locomotor function.

Editorial Comment

Protection by AMPA/Kainate Glutamate Receptor Antagonism

Pak H. Chan, PhD, Guest Editor

Neurosurgical Laboratories Stanford University Palo Alto, California

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