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(Stroke. 2001;32:1973.)
© 2001 American Heart Association, Inc.

Original Contributions

Chlamydia pneumoniae in Atherosclerotic Middle Cerebral Artery

Dezso Virok, MD; Zoltan Kis, MPH; Laszlo Karai, MD; Laszlo Intzedy, MD; Katalin Burian, MD; Arpad Szabo, MD; Bela Ivanyi, MD, PhD Eva Gonczol, MD, PhD

From the Department of Medical Microbiology (D.V., Z.K., K.B., E.G.), the Department of Pathology (L.K., L.I., B.I.), and the Department of Forensic Medicine (A.S.), University of Szeged, Szeged, Hungary, and The Wistar Institute (E.G.), Philadelphia, Pa.

Correspondence to Eva Gonczol, MD, PhD, Department of Medical Microbiology, University of Szeged, Dom ter 10, H-6720 Szeged, Hungary. E-mail gonczol{at}comser.szote.u-szeged.hu

Background and Purpose—— Atherosclerotic middle cerebral arteries are frequent sites of thrombosis, leading to stroke. Previous studies have suggested a role for Chlamydia pneumoniae in the pathogenesis of atherosclerosis. However, the presence of this pathogen in atherosclerotic middle cerebral arteries has heretofore not been documented. In the present study, we analyzed atheromatous plaques from middle cerebral arteries for the presence of C pneumoniae.

Methods—— Atherosclerotic middle cerebral arteries from 15 cadavers who died of natural causes and corresponding nonatherosclerotic arteries from 4 otherwise healthy trauma victims were examined. Assays for C pneumoniae DNA were carried out by nested polymerase chain reaction (nPCR) specific for the C pneumoniae ompA gene. The presence of the bacterium was assessed by transmission electron microscopy.

Results—— Five of the 15 atherosclerotic arterial samples and none of the control tissues were positive for C pneumoniae by nPCR. Particles similar in morphology and size to C pneumoniae elementary bodies were detected by transmission electron microscopy in 4 of the 5 nPCR-positive atherosclerotic samples.

Conclusions—— The demonstration of C pneumoniae in atherosclerotic middle cerebral arteries is consistent with the hypothesis that this bacterium is involved in acute and chronic cerebrovascular diseases.

Editorial Comment

Mitchell S. Elkind, MD; Guest Editor

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