This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Marquardt, L. Articles by Grau, A. J. Search for Related Content PubMed PubMed Citation Articles by Marquardt, L. Articles by Grau, A. J. Pubmed/NCBI databases Substance via MeSH Medline Plus Health Information Stroke Related Collections Secretion Aggregation Acute Cerebral Infarction Platelets

(Stroke. 2002;33:2570.)
© 2002 American Heart Association, Inc.

Original Contributions

Course of Platelet Activation Markers After Ischemic Stroke

Lars Marquardt, Cand Med; Andreas Ruf, MD; Ulrich Mansmann, PhD; Ralph Winter, MD; Matthias Schuler, MD; Florian Buggle, MD; Horst Mayer, MD Armin J. Grau, MD

From the Department of Neurology (L.M., F.B., A.J.G.), Institute of Medical Biometry and Informatics (U.M.), and Krankenhaus Bethanien, Geriatric Center (M.S.), University of Heidelberg; Center for Laboratory Medicine, Microbiology, Transfusion Medicine, Klinikum Karlsruhe (A.R., H.M.); and Department of Neurology, Kurpfalzkrankenhaus Heidelberg (R.W.), Heidelberg, Germany.

Correspondence to Armin J Grau, MD, Department of Neurology, University of Heidelberg, Im Neuenheimer Feld 400, 69120 Heidelberg, Germany. E-mail armin_grau{at}med.uni-heidelberg.de

Background and Purpose— The aim of this study was to evaluate the time course of platelet activation after ischemic stroke and to investigate whether platelet activation and inflammation are correlated with each other.

Methods— We serially determined expression of p-selectin (CD62p) and lysosome-associated membrane protein (CD63) by platelets using flow cytometry at 10 time points between days 1 and 90 in patients after ischemic stroke (n=50), in healthy subjects (n=30), and in risk factor control subjects (n=20). Furthermore, we correlated leukocyte count, C-reactive protein, and fibrinogen levels with platelet activation markers.

Results— CD62p and CD63 expression was higher on day 1 after stroke than in both control groups (P<0.005 for both). CD62p expression rapidly declined, whereas CD63 expression remained significantly elevated until day 90. Stroke severity and different medication for secondary stroke prevention did not influence CD62p or CD63 expression. Platelet activation markers and inflammatory parameters were not correlated with each other at any time point after stroke.

Conclusions— The initial increase in both CD62p and CD63 expression by platelets is followed by a differential regulation of both parameters after stroke. The rapid decrease in CD62p expression may be caused by shedding from the cell surface. Its persistent elevation makes CD63 a good candidate for studies on predictors for stroke recurrence. Our findings suggest that the expression of CD62p and CD63 by platelets is regulated independently from inflammatory indexes.

Key Words: flow cytometry • inflammation • platelets • stroke

This article has been cited by other articles:

				J. Smout, A. Dyker, M. Cleanthis, G. Ford, P. Kesteven, and G. Stansby Platelet Function Following Acute Cerebral Ischemia Angiology, June 1, 2009; 60(3): 362 - 369. [Abstract] [PDF]

				M. Cleanthis, J. Smout, V. Bhattacharya, H. Ashour, A. Dyker, G. Ashley Ford, D. P Mikhailidis, and G. P. Stansby Soluble but Not Platelet P-selectin Correlates With Spontaneous Platelet Aggregation: A Pilot Study Clinical and Applied Thrombosis/Hemostasis, April 1, 2008; 14(2): 227 - 233. [Abstract] [PDF]

				L. Navarro-Nunez, M. L. Lozano, J. Rivera, J. Corral, V. Roldan, R. Gonzalez-Conejero, J. A. Iniesta, J. Montaner, V. Vicente, and C. Martinez The association of the {beta}1-tubulin Q43P polymorphism with intracerebral hemorrhage in men Haematologica, April 1, 2007; 92(4): 513 - 518. [Abstract] [Full Text] [PDF]

				P. Htun, S. Fateh-Moghadam, B. Tomandl, R. Handschu, K. Klinger, K. Stellos, C. Garlichs, W. Daniel, and M. Gawaz Course of Platelet Activation and Platelet-Leukocyte Interaction in Cerebrovascular Ischemia Stroke, September 1, 2006; 37(9): 2283 - 2287. [Abstract] [Full Text] [PDF]

				J. P. Mathew, H. M. Rinder, B. R. Smith, M. F. Newman, and C. S. Rinder Transcerebral Platelet Activation After Aortic Cross-Clamp Release is Linked to Neurocognitive Decline. Ann. Thorac. Surg., May 1, 2006; 81(5): 1644 - 1649. [Abstract] [Full Text] [PDF]

				H. -K. Yip, C. -H. Lu, C. -H. Yang, H. -W. Chang, W. -C. Hung, C. -I. Cheng, S. -M. Chen, and C. -J. Wu Levels and value of platelet activity in patients with severe internal carotid artery stenosis Neurology, March 28, 2006; 66(6): 804 - 808. [Abstract] [Full Text] [PDF]

				H. S. Kirshner, J. Biller, and A. S. Callahan III Long-Term Therapy to Prevent Stroke J Am Board Fam Med, November 1, 2005; 18(6): 528 - 540. [Abstract] [Full Text] [PDF]

				M. T. Littleton-Kearney, J. M. Gaines, K. P. Callahan, S. J. Murphy, and P. D. Hurn Effects of Estrogen on Platelet Reactivity After Transient Forebrain Ischemia in Rats Biol Res Nurs, October 1, 2005; 7(2): 135 - 145. [Abstract] [PDF]

				J.A. Zeller, A. Lenz, C.C. Eschenfelder, P. Zunker, and G. Deuschl Platelet-Leukocyte Interaction and Platelet Activation in Acute Stroke With and Without Preceding Infection Arterioscler Thromb Vasc Biol, July 1, 2005; 25(7): 1519 - 1523. [Abstract] [Full Text] [PDF]

				S. K. Nadar, A. D. Blann, S. Kamath, D. G. Beevers, and G. Y. H. Lip Platelet indexes in relation to target organ damage in high-risk hypertensive patients: A substudy of the Anglo-Scandinavian Cardiac Outcomes Trial (ASCOT) J. Am. Coll. Cardiol., July 21, 2004; 44(2): 415 - 422. [Abstract] [Full Text] [PDF]

				H.-K. Yip, S.-S. Chen, J. S. Liu, H.-W. Chang, Y.-F. Kao, M.-Y. Lan, Y.-Y. Chang, S.-L. Lai, W.-H. Chen, and M.-C. Chen Serial Changes in Platelet Activation in Patients After Ischemic Stroke: Role of Pharmacodynamic Modulation Stroke, July 1, 2004; 35(7): 1683 - 1687. [Abstract] [Full Text] [PDF]

				M. Yamazaki, S. Uchiyama, A. J. Grau, L. Marquardt, and A. Ruf Platelet Function Under Aspirin, Clopidogrel, and Both After Ischemic Stroke: A Case-Crossover Study * Response Stroke, December 1, 2003; 34 (12): e227 - e228. [Full Text] [PDF]

				C. W. Kopp, S. Steiner, C. Nasel, D. Seidinger, I. Mlekusch, W. Lang, A. Bartok, R. Ahmadi, and E. Minar Abciximab Reduces Monocyte Tissue Factor in Carotid Angioplasty and Stenting Stroke, November 1, 2003; 34(11): 2560 - 2567. [Abstract] [Full Text] [PDF]

				M. Karakantza, A. Maniatis, C. I. Metallinos, T. Papapetropoulos, C. Paschalis, A. J. Grau, L. Marquardt, and A. Ruf In Vivo Platelet Activation in Ischemic Stroke Patients * Response Stroke, October 1, 2003; 34 (10): e174 - e175. [Full Text] [PDF]

				Tracking Platelet Activation After Ischemic Stroke Journal Watch Neurology, January 23, 2003; 2003(123): 2 - 2. [Full Text]