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(Stroke. 2003;34:2958.)
© 2003 American Heart Association, Inc.

Original Contributions

Neonatal Hypoxia-Ischemia Differentially Upregulates MAGUKs and Associated Proteins in PSD-93–Deficient Mouse Brain

Xiangning Jiang, MD, PhD; Dezhi Mu, MD, PhD; R. Ann Sheldon, MA; David V. Glidden, PhD Donna M. Ferriero, MD

From the Departments of Neurology (X.J., D.M., R.A.S., D.M.F.), Pediatrics (D.M., D.M.F.), and Epidemiology and Biostatistics (D.V.G.), University of California, San Francisco.

Correspondence to Donna M. Ferriero, MD, Department of Neurology, Box 0663, University of California, San Francisco, 521 Parnassus Ave, San Francisco, CA 94143–0663. E-mail dmf{at}itsa.ucsf.edu

Background and Purpose— Postsynaptic density (PSD)-93 and PSD-95 are the major membrane-associated guanylate kinases (MAGUKs) at excitatory synapses of the brain linking the N-methyl-D-aspartate receptor (NMDAR) with neuronal nitric oxide synthase (nNOS), which contributes to cell death after neonatal hypoxia-ischemia (HI). We investigated whether deletion of PSD-93 would dissociate the NMDAR from nNOS and be neuroprotective.

Methods— Postnatal day 7 wild-type (+/+), heterozygous (+/-), and homozygous (-/-) PSD-93 knockout mice were subjected to HI by permanent ligation of the right carotid artery, followed by exposure to 8% O₂/92% N₂ for 1 hour. Brains were scored 5 days later for damage with cresyl violet and iron stains. Western blot and coimmunoprecipitation were used to determine the expression and association of the major PSD proteins.

Results— There was no significant difference between PSD-93 (-/-) and (+/+) mice in mortality or degree of brain injury. In the absence of PSD-93, PSD-95 still interacted with NR2B and nNOS. Under physiological conditions, PSD-95, nNOS, NR2A, and NR2B were unaltered in the (-/-) pups. However, at 24 hours after HI, protein expression of PSD-95, nNOS, and NR2A but not NR2B was markedly higher in the (-/-) than in the (+/+) pups. In (+/+) pups, HI resulted in decreased expression of NR2A but not NR2B in cortex and decreased NR2A and NR2B expression in hippocampus, but this reduction was not observed in (-/-) pups.

Conclusions— PSD-93 is not essential for baseline synaptic function but may participate in regulation of NMDAR-associated signaling pathways after HI injury. Deletion of PSD-93 alone does not provide neuroprotection after neonatal HI, possibly a result, in part, of upregulation of PSD-95. MAGUKs may substitute for one another, allowing normal NMDAR function in the postnatal period.

Key Words: brain injuries • growth and development • mice • receptors, N-methyl-D-aspartate

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