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(Stroke. 2004;35:2206.)
© 2004 American Heart Association, Inc.

Original Contributions

Intracerebral Transplantation of Porcine Choroid Plexus Provides Structural and Functional Neuroprotection in a Rodent Model of Stroke

Cesar V. Borlongan, PhD; Steve J.M. Skinner, PhD; Marilyn Geaney, BS; Alfred V. Vasconcellos, BS; Robert B. Elliott, MD Dwaine F. Emerich, PhD

From the Department of Neurology, School of Medicine, and the Institute of Molecular Medicine and Genetics, School of Graduate Studies (C.V.B.), Medical College of Georgia, Augusta, Ga; the Research and Affiliations Service Line (C.V.B.), Augusta Veterans Affairs Medical Center, Augusta, Ga; LCT BioPharma, Inc (A.V.V, D.F.E.), Providence, RI; and Diatranz NZ Ltd/Living Cell Technologies (S.J.M.S., M.G., R.B.E.), Auckland, New Zealand.

Correspondence to Dr Cesar V. Borlongan, Department of Neurology, BI-3080, Medical College of Georgia, 1120 15th Street, Augusta, GA 30912-3200. E-mail cborlongan{at}mail.mcg.edu

Background and Purpose— Choroid plexus (CP) secretes a cocktail of neurotrophic factors. In the present study, CP from neonatal pigs was encapsulated within alginate microcapsules for in vitro and in vivo neuroprotective studies.

Methods— In vitro studies involved serum deprivation of rat embryonic cortical neurons and treatment with a range of concentrations of conditioned media from CP. For in vivo studies, rats received a 1-hour middle cerebral artery occlusion followed by intracranial transplantation of encapsulated or unencapsulated CP, empty capsules, or no transplant. Behavioral testing was conducted on days 1 to 3 after transplantation. Cerebral infarction was analyzed using 2,3,5-triphenyl-tetrazolium chloride staining at 3 days after transplantation.

Results— Conditioned media from CP produced a significant dose-dependent protection of serum-deprived cortical neurons. Enzyme-linked immunosorbent assay confirmed secretion of GDNF, BDNF, and NGF from CP. Parallel in vivo studies showed that CP transplants improved behavioral performance and decreased the volume of infarction. Both encapsulated and unencapsulated CP transplants were effective; however, more robust benefits accompanied encapsulated transplants.

Conclusions— These data are the first to demonstrate the neuroprotective potential of transplanted CP and raise the intriguing possibility of using these cells as part of the treatment regimen for stroke and other neurological disorders.

Key Words: cerebral ischemia • neuroprotection • stroke, acute

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