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(Stroke. 2007;38:153.)
© 2007 American Heart Association, Inc.

Original Contributions

Long-Lasting Regeneration After Ischemia in the Cerebral Cortex

Ronen R. Leker, MD; Frank Soldner, MD; Ivan Velasco, PhD; Denise K. Gavin, PhD; Andreas Androutsellis-Theotokis, PhD Ronald D.G. McKay, PhD

From the Laboratory of Molecular Biology (R.R.-L., R.S., I.V., D.K.G., A.A.-T., R.D.G.M.), National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD; and the Department of Neurology (R.R.L.), Hadassah University Medical Center, Jerusalem, Israel.

Correspondence to R.R. Leker, MD, Department of Neurology, Hadassah University Medical Center, Hadassah Ein Kerem, PO Box 12000, Jerusalem 91120, Israel. E-mail leker{at}cc.huji.ac.il

Background and Purpose— Because fibroblast growth factor 2 is a mitogen for central nervous system stem cells, we explored whether long-term fibroblast growth factor 2 delivery to the brain can improve functional outcome and induce cortical neurogenesis after ischemia.

Methods— Rats underwent permanent distal middle cerebral artery occlusion resulting in an ischemic injury limited to the cortex. We used an adeno-associated virus transfection system to induce long-term fibroblast growth factor 2 expression and monitored behavioral and histological changes.

Results— Treatment increased the number of proliferating cells and improved motor behavior. Neurogenesis continued throughout 90 days after the ischemia, and the occurrence of newly generated cells with characteristics of neural precursors and immature neurons was most evident 90 days after treatment.

Conclusions— Focal cortical ischemia elicits an ongoing neurogenic response that can be enhanced with fibroblast growth factor 2 leading to improved functional outcome.

Supplemental Materials and Methods