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(Stroke. 2008;39:1875.)
© 2008 American Heart Association, Inc.

Original Contributions

Granulocyte-Macrophage Colony-Stimulating Factor Enhances Leptomeningeal Collateral Growth Induced by Common Carotid Artery Occlusion

Kenichi Todo, MD; Kazuo Kitagawa, MD, PhD; Tsutomu Sasaki, MD, PhD; Emi Omura-Matsuoka, MD; Yasukazu Terasaki, MD; Naoki Oyama, MD; Yoshiki Yagita, MD, PhD Masatsugu Hori, MD, PhD

From the Division of Stroke, Department of Cardiovascular Medicine (K.T., T.S., E.O.-M., Y.T., N.O., M.H.), and Department of Neurology (K.K., Y.Y.), Osaka University Graduate School of Medicine, Japan.

Correspondence to Kenichi Todo, MD, Division of Stroke, Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan. E-mail ktodo{at}medone.med.osaka-u.ac.jp

Background and Purpose— Granulocyte-macrophage colony-stimulating factor (GM-CSF) has been reported to accelerate collateral growth (arteriogenesis) at the circle of Willis in rat brain. However, the effect of GM-CSF on leptomeningeal collateral growth has not been established. We examined the effect of unilateral common carotid artery (CCA) occlusion and GM-CSF treatment on leptomeningeal collateral growth in mice.

Methods— Adult mice were subjected to unilateral CCA occlusion or sham surgery followed by an alternate-day regimen of GM-CSF (20 µg/kg) or saline injection. On day 7, latex perfusion was performed in 1 set of mice to visualize the leptomeningeal vessels, and the number of Mac-2⁺ monocytes/macrophages on the dorsal surface of the brain was counted. In another set of mice, on day 7, permanent ipsilateral middle cerebral artery (MCA) occlusion was performed, and infarct volume was measured.

Results— Leptomeningeal collateral growth was observed after CCA occlusion, and that was enhanced by GM-CSF treatment. An increase in the number of Mac-2⁺ cells on the surface of the brain occurred after CCA occlusion and was enhanced by GM-CSF treatment. Seven days after CCA occlusion, GM-CSF treatment decreased the infarct size attributable to subsequent MCA occlusion.

Conclusion— After CCA occlusion, GM-CSF treatment enhanced leptomeningeal collateral growth and decreased the infarct size after MCA occlusion in mice.

Key Words: chronic ischemia • collateral perfusion • focal ischemia