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(Stroke. 2009;40:2165.)
© 2009 American Heart Association, Inc.

Original Contributions

Evaluation of MR-Derived Cerebral Oxygen Metabolic Index in Experimental Hyperoxic Hypercapnia, Hypoxia, and Ischemia

Hongyu An, DSc; Qingwei Liu, BS; Yasheng Chen, DSc Weili Lin, PhD

From Department of Radiology, University of North Carolina at Chapel Hill, Chapel Hill, NC.

Correspondence to Hongyu An, Department of Radiology, 106 Mason Farm Road, CB#7515, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599. E-mail hongyuan{at}med.unc.edu

Background and Purpose— A noninvasive MRI method to measure cerebral oxygen metabolism has the potential to assess tissue viability during cerebral ischemia. The purposes of this study were to validate MR oxygenation measurements across a wide range of global cerebral oxygenation and to examine the spatiotemporal evolution of oxygen metabolism during focal middle cerebral artery occlusion in rats.

Methods— A group of rats (n=28) under normal, hyperoxic hypercapnia and hypoxia were studied to compare MR-measured cerebral oxygen saturation (O₂Sat_MRv) with blood gas oximetry measurements in the jugular vein (O₂Sat_JV) and superior sagittal sinus (O₂Sat_SSS). In a separate group of rats (n=31), MR-measured cerebral oxygen metabolic index (MR_COMI) was acquired at multiple time points during middle cerebral artery occlusion. Histogram analysis was performed on the normalized MR_COMI (rMR_COMI) to examine evolution of oxygen metabolism during acute ischemia.

Results— Highly linear relationships were obtained between O₂Sat_MRv and O₂Sat_JV/O₂Sat_SSS in rats under global cerebral oxygenation alterations. In the focal ischemia study, rMR_COMI values were significantly lower within the areas of eventual infarction than other regions. Moreover, the rMR_COMI values within the ischemic territory decreased with time, concomitant with an increase in the number of voxels with severely impaired oxygen metabolism.

Conclusion— Accurate estimates of O₂Sat_MRv can be obtained across a broad and physiologically relevant range of cerebral oxygenation. Furthermore, this method demonstrates a dynamic alteration of cerebral oxygen metabolism during acute ischemia in rats.

Key Words: cerebral oxygen metabolism • hyperoxic hypercapnia • hypoxia • ischemic stroke