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(Stroke. 1975;6:691.)
© 1975 American Heart Association, Inc.

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Effects of Oxygen Saturation and Pco₂ on Brain Uptake of Glucose Analogues in Rabbits

FRANK G. BERSON M.D.¹; MARIA SPATZ M.D.¹; IGOR KLATZO M.D.¹

¹ Laboratory of Neuropathology and Neuroanatomical Sciences, National Institute of Neurological and Communicative Disorders and Stroke, Building 36, Room 4D-04, National Institutes of Health, Bethesda, Maryland 20014

The effect of oxygen saturation and Pco₂ on brain uptake of glucose analogues was studied in rabbits. Using a modified Oldendorf technique, ¹⁴C-labeled glucose analogues with a ³H₂O reference standard were introduced into the cerebral circulation via the common carotid artery, and the radioactivity of the ipsilateral cerebral cortex was counted and expressed in terms of a brain uptake index (BUI).

Severe hypoxia (oxygen saturation 18%) resulted in approximately a 40% decrease in the BUI of 2-deoxy-D-glucose and a 45% decrease in the BUI of 3-0-methyl-D-glucose. Severe hypercapnia (Pco₂, = 100 mm Hg) caused a 45% decrease in the BUI of both of these glucose analogues. Hypercapnia superimposed on severe hypoxia had no additional effect. Hypocapnia (Pco₂ = 15 mm Hg) increased the BUI of 3-0-methyl-D-glucose by 35% of the control value, and this increase was extremely sensitive to competitive inhibition. When BUI values were plotted against pH rather than Pco₂, for the same experiments, there was a good correlation with the calculated linear regression.

These results are compared with previous findings on pathologically induced changes in brain uptake of glucose analogues, and the possible role of blood flow is considered in detail.

Key Words: hypoxia • hypercapnia • hypocapnia • hyperoxia • glucose analogue transport across blood-brain barrier