(Stroke. 2000;31:3041.)
© 2000 American Heart Association, Inc.
Original Contributions |
From the Departments of Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Md.
Correspondence to Patricia D. Hurn, PhD, Blalock 1404, Johns Hopkins Hospital, 600 N Wolfe St, Baltimore, MD 21287. E-mail phurn{at}jhmi.edu
| Abstract |
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MethodsOvariectomized female Wistar rats received LY 10 mg/kg (n=16) or an equivalent volume of vehicle (n=14) by gavage for 5 to 8 days. Subsequently, each animal was anesthetized with halothane (1.2%) and treated with 2 hours of MCA occlusion by the intraluminal filament technique and 22 hours of recovery. Infarction volumes in the cerebral cortex and caudoputamen were determined by 2,3,5-triphenyltetrazolium chloride staining and digital image analysis. End-ischemic regional cerebral blood flow (CBF) was measured in separate animal cohorts by quantitative [14C]iodoantipyrine autoradiography.
ResultsCaudoputamen infarction was reduced by LY treatment (49±6% versus 64±4% of ipsilateral caudoputamen in LY and vehicle groups, respectively; P<0.05). Cerebral cortical infarction was not different in the LY compared with vehicle group (7±3% versus 13±4% of ipsilateral cerebral cortex, respectively). Intra-ischemic blood pressure, arterial blood gases, and temporalis muscle temperature were controlled and equivalent between treatment groups. Averaged laser-Doppler flow during MCA occlusion was 36±3% of baseline in the LY group versus 29%±2% in the vehicle group. However, end-ischemic CBF or blood flow distribution within the MCA territory was not altered by LY treatment. Cortical or caudoputamen tissue volumes with end-ischemic CBF <20 mL/100 g per minute were similar in both groups.
ConclusionsWe conclude that LY confers neuroprotection from focal cerebral ischemia in caudoputamen in ovariectomized female rats. The mechanism of protection is not linked to preservation of ischemic cerebral blood flow, as determined by end-occlusion quantitative autoradiography.
Key Words: cerebral infarction cerebral ischemia, focal estrogens hormones stroke women
| Introduction |
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A real concern for women who hesitate to begin estrogen replacement therapy, but who have known stroke risk factors, is the link of estrogen to increased risk of thromboembolism25 and breast26 27 and endometrial cancer.28 29 30 Additionally, while the risk of endometrial cancer can be reduced by coadministration of progestin with estrogen, it is unclear whether combined therapy alters the efficacy of estrogen in cardiovascular disease or stroke. Thus, the development of a new class of drugs, selective estrogen receptor modulators (SERMs), has been pursued to enhance estrogen agonist activity in the cardiovascular system and in bone, while causing either antagonist or no effect in breast and endometrium. LY353381.HCl (LY), or 2-(4-methoxyphenyl)-3-[4-[2-(1-piperidinylethoxy]phenoxy]benzo[b]thiophene-6-ol hydrochloride, is a novel benzothiophene analogue with structure and in vivo SERM activity that is similar but not identical to raloxifene (for structural modifications, see References 31 and 32 ). It prevents ovariectomy-associated weight gain, serum cholesterol elevation, and bone mineral loss, while it preserves estrogen antagonist effects on the uterus. This new SERM is of interest because of its enhanced potency relative to previously studied nonsteroidal template compounds, its excellent oral bioavailability, and its penetration into brain.31 32 The purpose of this study was to determine potential neuroprotective effects of LY in experimental middle cerebral artery (MCA) occlusion in ovariectomized female rats. Additionally, we measured regional CBF during MCA occlusion to assess potential effects on preservation of intraischemic CBF.
| Materials and Methods |
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Focal cerebral ischemia was accomplished by modification of the intraluminal filament method for proximal MCA occlusion.18 In brief, each animal was anesthetized with halothane (5% induction, then 1% to 1.5%) via nose cone with supplemental oxygen and air. A femoral artery was cannulated for blood pressure monitoring and arterial blood gas sampling. Rectal and temporalis muscle temperatures were measured and controlled with use of a heating lamp. The rat was placed on a stereotaxic frame equipped with a snout mask, a laser-Doppler flowmetry (LDF) probe holder, and a modification to allow for rotation about the longitudinal axis of the rat. An area 2 to 3 mm in diameter was thinned in the right parietal bone 2 mm posterior and 6 mm lateral to bregma for LDF (model MBF3D, Moore Instruments, Ltd). The LDF probe was positioned in its cranial window for semicontinuous measurements during vascular occlusion and the first 15 minutes of reperfusion. The right common carotid artery was exposed via a lateral neck incision, separated from the vagus nerve, and ligated. The external carotid artery was ligated, the occipital branch was cauterized, and the pterygopalatine artery was exposed and ligated. A 4-0 monofilament surgical suture with a heat-rounded tip was advanced through the common carotid artery and advanced into the internal carotid artery until an abrupt reduction in LDF signal was observed. The filament was secured in place. LDF was recorded at 5, 15, 30, 60, 90, and 120 minutes of MCA occlusion. Then the suture was withdrawn to initiate reperfusion, which was confirmed by LDF. Wounds were closed, and the animal was awakened. At 22 hours of reperfusion, the rat was reanesthetized with halothane, and the brain was harvested and sliced into seven 2-mm-thick coronal sections for 2,3,5-triphenyltetrazolium chloride staining. Infarction volumes were measured with the use of digital photography and image analysis software (SigmaScan Pro, Jandel). The infarcted area was numerically integrated across each section and over the entire ipsilateral hemisphere. Cortical and caudoputamen infarction was analyzed as a percentage of the volume of that ipsilateral cortex and caudoputamen, respectively.
End-ischemic regional CBF was measured in additional cohorts of Wistar rats that did not survive by quantitative autoradiography with [14C]iodoantipyrine (IAP), as described previously.18 Femoral vascular catheters were placed, and the MCA was occluded as in the previous cohorts. At 2 hours of MCA occlusion, arterial blood pressure and blood gases were measured, then 40 µCi of [14C]IAP (New England Nuclear) in 0.8 mL of isotonic saline was infused intravenously over 45 seconds. During infusion, fifteen 10-µL samples of free-flowing arterial blood from the femoral artery catheter were collected in heparin-coated sample tubes. With the filament still in place and LDF confirming the ischemic status, the rat was decapitated 45 seconds after the start of infusion. One postdecapitation arterial blood sample was collected. The brain was quickly removed and frozen at -50°C in 2-methylbutane on dry ice. Each brain was sectioned by cryostat into 20-µm-thick coronal sections at -20°C and thaw-mounted onto cover glasses. For 1 week, sections and 14C standards were apposed to film (Kodak, Bio-Max MR). The concentrations of [14C]IAP in blood samples was determined by liquid scintillation spectroscopy (model 3801, Beckman) after decolorization with 0.2 mL of tissue solubilizer (Soluene-350, Packard Instruments Co). Autoradiographic images representing 5 different coronal levels (+2.2, +0.2, -1.8, -3.8, and -5.8 mm from bregma, 6 to 9 images each) were digitized, and regional CBF was determined with the use of image analysis software (Inquiry, Loats Associates). Rates of regional CBF were calculated as previously described.18 24
Two methods of analysis were used to evaluate end-ischemic CBF within MCA territory ipsilateral and contralateral to occlusion. First, CBF was measured by sampling 0.08-mm2 squares within gray matter of those regions most vulnerable to MCA occlusion, the frontal and parietal lobes of cerebral cortex and the medial and lateral aspects of caudoputamen. Flow rates were then averaged from squares assayed from 6 to 9 consecutive brain slices at each of 3 coronal levels (+2.2, +0.2, and -1.8 mm from bregma). In the second method, areas categorized by predetermined intervals of CBF were isolated by digital image scanning and summed to construct a histogram distribution of brain tissue over incremental ranges of CBF. Areas were averaged from 3 images from each of 5 coronal levels (+2.2, +0.2, -1.8, -3.8, -5.8 mm from bregma) and then were numerically integrated to obtain an estimate of tissue volume for each incremental range of CBF.
All values are reported as mean±SE. Physiological parameters and LDF over the ischemic and early reperfusion interval were analyzed by 2-way ANOVA. When there was a significant effect of group, post hoc comparisons were made between groups at each time point with 1-way ANOVA and subsequent Newman-Keuls test. Differences in infarct size, mean residual laser-Doppler flow, and autoradiographic regional CBF among groups were determined with 1-way ANOVA. The criterion for statistical significance was P<0.05.
| Results |
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To evaluate cortical and subcortical perfusion during MCA occlusion,
absolute CBF and blood flow distribution within the MCA territory were
quantified in an additional animal cohort (LY, n=4; vehicle, n=3).
End-ischemic regional CBF in frontal and parietal cortex was
not different in LY- and vehicle-treated groups (Figure 3
). Blood flow to medial and lateral
sections of caudoputamen was also similar in both treatment
groups. Furthermore, when brain volume was partitioned into blood flow
increments throughout the ischemic hemisphere, there was no
difference in blood flow distribution during vascular occlusion (Figure 4
). LY treatment did not alter the
distribution of tissue volume into low flow zones (eg, <20 mL/100 g
per minute), which represent the infarct core.
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| Discussion |
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SERMs represent a new class of compounds that possess
tissue-specific estrogen receptor binding and elicit agonist or
antagonist effects in different target
tissues.33 34 The precise mechanisms by which SERMs such
as tamoxifen or raloxifene act as estrogenic versus antiestrogenic
molecules are actively under investigation and unclear at present.
Various compounds within the class differ by relative binding affinity
for known estrogen receptor subtypes (
versus ß) and by chemical
structure, which determines the unique conformation of receptor/ligand
complex.35 Putative molecular mechanisms by which a SERM
acts as an estrogen agonist include interaction with
coactivator proteins within DNA transcription complexes,
engagement of alternative docking sites within estrogen response
elements in genes, or differential binding to the ß receptor
subtype.36 37 38 In rats, LY is an orally active
benzothiophene compound that potently prevents ovariectomy-induced
effects on body weight, serum cholesterol, and bone
density. It is effective in bone mineral preservation, serum
cholesterol reduction, and reduction of atherosclerotic
pathology, while it maintains estrogen antagonist activity
in uterus.31 32 To our knowledge, this is the first study
to demonstrate a potential neuroprotective effect of a SERM in cerebral
ischemia.
Our present finding of reduced infarction volume limited to
caudoputamen in LY-treated animals differs from the
protection we have previously observed with 17ß-estradiol in this
same model. Chronic pretreatment with 17ß-estradiol at
physiologically relevant doses reduces damage
to cortex as well as caudoputamen in both young
ovariectomized19 and reproductively
senescent24 female rats. This regional difference may be
related to true differences in neuroprotective potential of the 2
agents or may be a function of a less than optimal dosing regimen for
LY. The optimal dose of LY for either agonist or antagonist
activity within the central nervous system is not known. Our rationale
for dose (10 mg/kg daily) and route (by gavage) was based on previous
work demonstrating potency and efficacy in other responsive targets
such as uterus31 and bone.32 The regionally
restricted efficacy of LY to caudoputamen versus cortex may
also be a reflection of the small amount of cortical damage exhibited
in animals regardless of treatment assignment. Because damage was small
in cortex relative to caudoputamen, treatment effects would
be difficult to demonstrate even with the reasonably robust animal
cohort size used in this study (n=14 to 16 per group). However, we
cannot exclude the possibility that LY interacts differently with
available estrogen receptor subtypes in cortex relative to
17ß-estradiol. Both
and ß estrogen receptor subtypes have been
identified in rat cortex.39
We investigated effects of LY on CBF in the contralateral, nonischemic, and ipsilateral hemispheres to determine whether the compound enhanced regional perfusion. Contralateral blood flow was unchanged by chronic LY treatment compared with vehicle, suggesting a lack of basal CBF augmentation under conditions of halothane anesthesia. Both absolute CBF and CBF distribution were also similar in vehicle- and drug-treated animals, demonstrating that mitigation of intraischemic blood flow defects was not a primary mechanism by which LY reduced injury. This finding is similar to our previous observations with 17ß-estradiol in this ischemic model, in which exogenous estrogen appears to exert flow-independent neuroprotection.19 It should be noted that these data during MCA occlusion do not exclude possible CBF enhancement during reperfusion. Nevertheless, the present results indicate that for an equivalent reduction of CBF during vascular occlusion, LY was efficacious in salvaging tissue within the caudoputamen at 24 hours after MCA occlusion.
Whether SERMs act directly on neurons or glia to provide
ischemic protection is not known. It seems likely that multiple
mechanisms are engaged, in much the same manner as 17ß-estradiol is
hypothesized to act in neuroinjury. Accumulating evidence implicates
estrogen receptor-independent antioxidant properties and free radical
scavenging,40 receptor-dependent reduction of calcium
current inflow,41 inhibition of glutamate and
N-methyl-D-aspartate
toxicity,42 43 44 and preservation of protective gene
products such as bcl-2.45 46 Recent studies suggest
that the neuroprotection of estrogen in experimental stroke is not
mediated via estrogen subtype
receptors.47 The
importance of the ß subtype in neuronal survival has been suggested
and is under active investigation.48
In summary, we have demonstrated that pretreatment with LY reduces caudoputamen infarction volume at 24 hours in a standard model of MCA occlusion in ovariectomized female rats. The protection is not dependent on augmentation of ischemic CBF and enhanced vasodilation during vascular occlusion. However, effects on recovery of CBF during reperfusion cannot be excluded. Further study is warranted to explore the potential clinical utility of LY in neuroinjury and to more fully elucidate its mechanisms of cerebral protection.
| Acknowledgments |
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Received May 16, 2000; revision received August 9, 2000; accepted August 18, 2000.
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Office of the Vice President for Health Sciences Medical College of Virginia Virginia Commonwealth University Richmond, Virginia
| Introduction |
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The article by Rossberg and colleagues examined the effect of a selective estrogen receptor modulator, LY353381, in experimental ischemic stroke. The authors found that this experimental drug decreased infarct size in the caudoputamen but did not reduce infarct size in the cerebral cortex. This effect differs from that of estrogen, which in earlier experiments reduced infarct size in both locations.
It is not known whether this represents a less-potent effect of LY353381 than estrogen or whether this is the result of the experimental conditions. It should be noted that the percentage reduction of the infarct size in the cerebral cortex was not significant, although numerically it was greater than that in the caudoputamen (50% versus 23%, respectively). The absence of statistical significance might be related to the fact that infarct size in this model in the cerebral cortex is small: 13%, versus 64% in the caudoputamen). Also, a single dose of the drug was used, which may not have been optimal.
Accordingly, although these initial results are encouraging, additional work is necessary, using different doses of the drug and also probably testing it in different models of ischemic stroke.
Received May 16, 2000; revision received August 9, 2000; accepted August 18, 2000.
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