This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Johnston, S. C. Articles by Dean, D. Search for Related Content PubMed PubMed Citation Articles by Johnston, S. C. Articles by Dean, D. Pubmed/NCBI databases Substance via MeSH Medline Plus Health Information Carotid Artery Disease Related Collections Mechanism of atherosclerosis/growth factors Pathophysiology Risk Factors Carotid Stenosis Risk Factors for Stroke

(Stroke. 2001;32:2748.)
© 2001 American Heart Association, Inc.

Original Contributions

C-Reactive Protein Levels and Viable Chlamydia pneumoniae in Carotid Artery Atherosclerosis

S. Claiborne Johnston, MD, PhD; Louis M. Messina, MD; Warren S. Browner, MD, MPH; Michael T. Lawton, MD; Caroline Morris, RN, CNS Deborah Dean, MD, MPH

From Neurovascular Service, Department of Neurology (S.C.J., C.M.); Division of Vascular Surgery, Department of Surgery (L.M.M.); Department of Neurological Surgery (M.T.L.); and Division of Infectious Diseases, Department of Medicine (D.D.), University of California, San Francisco; California Pacific Medical Center Research Institute, San Francisco (W.S.B.); and Children’s Hospital of Oakland Research Institute, Oakland, Calif (D.D.).

Correspondence to S. Claiborne Johnston, MD, PhD, Department of Neurology, Box 0114, University of California, San Francisco, 505 Parnassus Ave, M-798, San Francisco, CA 94143-0114. E-mail clayj{at}itsa.ucsf.edu

	Abstract

Top Abstract Introduction Subjects and Methods Results Discussion References

 
Background and Purpose— An elevated serum level of C-reactive protein, an inflammatory marker, is an independent predictor of stroke and coronary artery disease. To determine whether chronic infection with Chlamydia pneumoniae, which has been identified in atherosclerotic plaques, is responsible for systemic inflammation, we studied the association between serum C-reactive protein levels and infection of carotid artery atherosclerotic plaque with viable C pneumoniae.

Methods— Serum C-reactive protein levels were obtained before endarterectomy for carotid artery stenosis. Plaques were tested for C pneumoniae mRNA, an indicator of viability, and DNA by polymerase chain reaction of DNA and cDNA, respectively.

Results— Forty-eight samples were studied, of which 18 (38%; 95% CI, 23 to 50) were infected with viable C pneumoniae as evidenced by isolated chlamydial mRNA. All 18 of these samples, plus 1 additional sample, were positive for chlamydial DNA. Serum C-reactive protein levels were higher in those with viable C pneumoniae compared with those without infection (median, 8 mg/L versus undetectable; P=0.045 by Wilcoxon rank-sum test). In multivariable models, the only independent predictor of the presence of viable C pneumoniae was a detectable C-reactive protein level (odds ratio, 4.2; 95% CI, 1.1 to 17; P=0.04).

Conclusions— Viable C pneumoniae are present in a substantial portion of carotid artery atherosclerotic plaques and are associated with increased serum C-reactive protein levels. These findings may explain the link between elevated C-reactive protein levels and the risk of cardiovascular disease and stroke but should be reproduced in a larger cohort.

Key Words: atherosclerosis • carotid arteries • Chlamydia pneumoniae • C-reactive protein • inflammation

	Introduction

Top Abstract Introduction Subjects and Methods Results Discussion References

 
C-reactive protein is a sensitive indicator of acute and chronic inflammation.¹ In apparently healthy men and women, an elevated serum level of C-reactive protein is a predictor of long-term risk of myocardial infarction and stroke.^2–4 It is also an independent predictor of the risk of cardiovascular events in patients with coronary artery disease.⁵ The association between serum C-reactive protein levels and subsequent risk of stroke, myocardial infarction, and death from cardiac causes supports the importance of inflammation in the pathogenesis of cerebrovascular and coronary artery disease.^5,6

Focal inflammation is present in atherosclerotic plaques and has been associated with the progression of vascular stenosis and its complications.^6–9 Chronic infection of arteries has been proposed as a cause of inflammation and a contributor to the initiation and progression of atherosclerosis. Polymerase chain reaction (PCR), immunocytochemical staining, and electron microscopy are among the techniques that have demonstrated evidence of Chlamydia pneumoniae infection in coronary and carotid artery plaques.^10–16 Detection of viable organisms, a clearer indicator of ongoing infection, has been more difficult. In 4 studies, viable C pneumoniae has been cultured from a small proportion of atherosclerotic plaques.^12,14,17,18 In another study, evidence of viable C pneumoniae was found in 10 of 30 patients by isolation of RNA and reverse-transcriptase (RT) PCR.¹⁹

Chronic infection of blood vessels with C pneumoniae could lead to elevation of C-reactive protein levels and contribute to the instability or progression of atherosclerotic plaques.^20–23 Several studies have found an association between C-reactive protein and serological evidence of C pneumoniae infection, whereas others have not.^24–29 These variable results may be explained by poor correlation between positive chlamydial serologies and evidence of plaque infection.^12,16,18,30 One study compared C-reactive protein levels in patients with and without PCR evidence of C pneumoniae in atherosclerotic plaques and failed to find a statistically significant difference.¹⁶ Thus, the connection between C pneumoniae plaque infection, systemic inflammation, and symptomatic vascular disease remains uncertain. To determine whether viable C pneumoniae organisms are present in atherosclerotic plaques and whether these organisms are associated with inflammation and the risk of stroke, we evaluated vascular risk factors, signs and symptoms of infection, preoperative serum C-reactive protein levels, and presence of viable C pneumoniae in carotid tissue in a prospective study of patients undergoing elective carotid endarterectomy.

	Subjects and Methods

Top Abstract Introduction Subjects and Methods Results Discussion References

 
Subjects
Between March 1998 and August 2000, all patients scheduled for elective carotid endarterectomy with preoperative evaluations at the University of California, San Francisco, were approached for participation in the study, which was approved by the local institutional review board for human research. All those providing consent from whom carotid plaque was available were enrolled. A clinician completed a structured interview to obtain information on risk factors for atherosclerosis (including elevated cholesterol, hypertension, diabetes, obesity, coronary artery disease, and smoking history), recent use of antibiotics, and signs and symptoms that might be considered compatible with C pneumoniae infection (questionnaire available at www.neurology.ucsf.edu), although these infections may be asymptomatic in many cases. Medical records were reviewed to obtain information about medical history and medications during the prior year and at presentation.

Symptomatic carotid arteries were defined as those associated with stroke or transient ischemic attack. An operation on a previously treated artery was defined as a repeated endarterectomy. Antibiotics considered effective in treating C pneumoniae included tetracycline, doxycycline, erythromycin, clarithromycin, azithromycin, and ofloxacin. Symptoms and diagnoses of infection and antibiotic usage were recorded if they occurred during the year before surgery. Cough, sore throat, nasal congestion, and sinus congestion were defined as symptoms of upper respiratory infection. Respiratory infections included diagnoses of common cold, flu, pneumonia, bronchitis, and sinusitis.

C-Reactive Protein Assay
All blood samples were drawn before surgery. C-reactive protein levels were determined with fixed-rate nephelometry (Nichols Institute, Quest Diagnostics), the most sensitive test available at study initiation; the technique is sensitive to levels >4 mg/L.³¹ Levels below the detection threshold (<4 mg/L) were defined as not detectable.

Carotid Samples
Atherosclerotic tissue was removed by the operating physician using standard surgical techniques. The tissue was sectioned immediately through the center of the widest portion of arterial wall. One section was placed in a sterile Eppendorf tube and another in a tube containing 500 µL RNAzol B (Tel-Test, Inc). Both vials were stored at -80°C until processed. DNA was extracted and purified as previously described.^32–34 We performed PCR to identify chlamydial infection in carotid atherosclerotic plaques using genus-specific primers to detect any of the 3 chlamydial species (C trachomatis, C pneumoniae, or C psittaci). We used nested PCR in positive-PCR specimens to determine the chlamydial species present.^33–35 To confirm appropriate size, PCR products were compared with molecular-weight markers by electrophoresis on an agarose gel.

Messenger RNA was extracted from the tissue samples in accordance with the manufacturer’s protocol (Tel-Test, Inc) and subjected to RT-PCR to generate cDNA for PCR amplification.³⁶ The cDNA was subjected to PCR and agarose gel size verification as described above. In addition, primers specific for HPRT, a housekeeping gene, were used in PCR for each cDNA sample to determine the adequacy of the sample; all samples were amplified by these primers. Quantification of bacterial load was measured in all samples positive for C pneumoniae by use of competitive PCR with known amounts of a polycompetitor cDNA containing C pneumonia. The polycompetitor contains an insert that increases its molecular weight compared with the wild type.³⁷ Briefly, the polycompetitor was used in concentrations of 1, 10, 50, and 100 copies and run with each batch of patient samples for the PCR. A master mix was created that included all the ingredients for PCR except for the patient and polycompetitor samples. The master mix was divided equally among these samples; the respective sample cDNA or polycompetitor sample was added to the respective tube; and all tubes were subjected to the same PCR thermocycling parameters at the same time. Ten microliters of each patient and polycompetitor sample was run on a 1.5% agarose gel stained with ethidium bromide. The quantity of cDNA in the sample was determined by comparison with the results for the 4 concentrations of polycompetitor with the use of a Bio-Rad gel documentation system according to the manufacturer’s instructions. Samples that contained 1 copy of mRNA in the quantitative assay were considered to have viable organisms.

Statistical Analysis
Dichotomous variables were compared with Pearson’s ² test or Fisher’s exact test when any cell of a 2x2 table was <5. Exact 95% CIs for prevalent C pneumoniae were calculated by use of exact binomial probabilities. C-reactive protein levels, age, and pack-years of smoking were compared with the Wilcoxon rank-sum test, because these variables were not normally distributed. Multivariable logistic regression analysis was performed, including all variables associated with the presence of viable C pneumoniae (at P<0.20) and removing those no longer contributing (at P>0.10) in a stepwise manner. All statistical analyses were performed with the STATA statistical package (version 6.0).

	Results

Top Abstract Introduction Subjects and Methods Results Discussion References

 
Seventy-four patients were scheduled for elective carotid endarterectomy during the study period. Scheduling prevented enrollment of 26 patients (most commonly because the carotid plaque was removed when laboratory staff was unavailable); 1 patient refused consent; and plaque could not be removed intact in 2 others.

A total of 48 endarterectomy samples were obtained from 46 patients (mean±SD age, 72±8 years), 14 (30%) of whom were female. In 15 patients (31%), the treated carotid artery was associated with symptoms caused by stroke or transient ischemic attack; the remaining patients were asymptomatic. The procedure was a reoperation in 4 patients (8%). Vascular risk factors were common, including smoking (n=37), hypercholesterolemia (n=33), hypertension (n=31), and diabetes mellitus (n=10). Of the 44 patients who provided information on signs and symptoms of infection, 35 (80%) identified a diagnosis or symptoms of infection in the year before endarterectomy, most frequently consistent with respiratory infection (n=32, 73%).

Serum C-reactive protein levels before endarterectomy ranged from undetectable to 86 mg/L, with a median below the detection threshold; 21 patients had detectable levels. Detectable C-reactive protein levels were associated with a history of vomiting, diarrhea, or the taking of antibiotics in the prior year (the Table).

View this table: [in this window] [in a new window]   Table 1. Characteristics of Patients by Presence of Viable C pneumoniae and Detectable Serum C-Reactive Protein

PCR revealed evidence of C pneumoniae in 19 carotid plaques (40%). Eighteen of these showed evidence of viable C pneumoniae (38%; 95% CI, 23 to 50) on the basis of the presence of chlamydial RNA. Two patients had bilateral endarterectomies; 1 had evidence of viable C pneumoniae in both plaques, whereas neither plaque from the other patient was infected. Patients with viable C pneumoniae were somewhat more likely to report signs and symptoms of infections in the prior year (the Table).

Serum C-reactive protein levels were greater in patients with viable C pneumoniae than in those without evidence of viable chlamydial organisms (median, 8 mg/L versus not detectable; P=0.045; the Figure). Viable C pneumoniae were twice as common in those with detectable serum C-reactive protein levels than in other patients [52% (11 of 21) versus 26% (7 of 27); P=0.06]. In multivariable models, the only independent predictor of the presence of viable C pneumoniae was a detectable C-reactive protein level (odds ratio, 4.2; 95% CI, 1.1 to 17; P=0.04); the presence of signs or symptoms of infection in the prior year was retained in the model but was not a significant predictor (odds ratio, 6.6; 95% CI, 0.7 to 64; P=0.10). No other demographic characteristics or vascular risk factors were predictors. The presence of viable C pneumoniae was not associated with neurological symptoms at presentation.

View larger version (15K): [in this window] [in a new window]   C-reactive protein level by presence of viable C pneumoniae as detected by PCR of cDNA generated by RT-PCR of mRNA isolated from carotid artery atherosclerotic plaques. Median serum C-reactive protein levels (solid lines) were greater for those with viable C pneumoniae (P=0.045). Dashed line represents the detection limit of the test.

	Discussion

Top Abstract Introduction Subjects and Methods Results Discussion References

 
Our study of atherosclerotic carotid arteries suggests that chronic infection with viable C pneumoniae is common: 40% of plaques removed at endarterectomy had evidence of viable organisms. Infection appeared to be unrelated to vascular risk factors, signs and symptoms suggestive of infection, or presentation with stroke or transient ischemic attack. Similar to results of prior studies,^12,15,20 we found that vascular infection with C pneumoniae appears to be asymptomatic.

Other groups have demonstrated viable C pneumoniae from cultures of atherosclerotic plaques in the coronary,^12,18 femoral,¹⁷ and carotid arteries,^14,17 albeit in only 4% to 16% of specimens. A single study used RT-PCR in carotid endarterectomy tissue and found evidence of viable C pneumoniae in 10 of 30 patients,¹⁹ a detection rate much greater than those seen in studies that used culture. We found a similar rate of infection, which was corroborated by PCR of independently extracted DNA. Furthermore, we showed that plaques with viable infections contained a large number of organisms, suggesting that these infections could be clinically relevant. Characteristics of the referral population we studied—largely non-Hispanic white and referred for elective treatment—may have influenced the prevalence of C pneumoniae we found.

This is the first study to demonstrate higher serum C-reactive protein levels in patients with viable carotid C pneumoniae. This association may explain why the C-reactive protein level is a risk factor for stroke and cardiovascular events: Higher levels may indicate chronic arterial wall infection with C pneumoniae, which in turn may contribute to plaque progression.^20–23 Recent in vitro studies have shown that C pneumoniae infection of human smooth muscle cells results in production of interleukin-6,³⁸ which is the major regulator of C-reactive protein production,³⁹ providing a plausible pathophysiological link.

Our findings could also be explained by an unrecognized factor that increases C-reactive protein levels and increases the risk of C pneumoniae infection. For example, an underlying susceptibility to infection could be associated with both elevated C-reactive protein levels and C pneumoniae infection without a causal link between the 2 factors. We did not identify such a factor in this study, but our sample was small.

Others have studied C-reactive protein in C pneumoniae infection, but they have not attempted to detect viable organisms. One study that compared C-reactive protein levels in those with and without PCR evidence of C pneumoniae DNA¹⁶ reported that mean levels were higher (but not significantly so) in those who were PCR positive. Several groups have studied the association between C-reactive protein and positive serologies for C pneumoniae, with conflicting results.^24–29 Recent studies have shown, however, that serology is a poor marker for the presence of C pneumoniae DNA or RNA.^12,16,18,30

If chronic vascular infection with C pneumoniae is responsible for C-reactive protein elevation, treatment should be associated with reduced C-reactive protein levels. Three small trials have tested whether antibiotics directed at C pneumoniae reduce vascular events and inflammatory markers in high-risk patients. All 3 studies demonstrated reductions in C-reactive protein levels in those treated with antibiotics,^40–42 and 1 study demonstrated a reduction in vascular events.⁴⁰ The reduction in C-reactive protein after treatment could be due to eradication of C pneumoniae or, alternatively, of other infectious agents treated by these antibiotics.

The association that we found between the presence of viable C pneumoniae and detectable levels of C-reactive protein should be reproduced in a larger group of patients. More sensitive tests for C-reactive protein are now available and could provide additional information, particularly because cohort studies have shown that differences in levels below the detection limit of our assay are associated with the risk of cardiovascular events.^3,4 The fact that we detected a difference with a less sensitive, first-generation test suggests that the association between levels of C-reactive protein and the presence of viable C pneumoniae is strong. However, because the positive predictive value of a detectable C-reactive protein level is low with the less sensitive test, it should not be used as a screening test to identify those with viable C pneumoniae.

	Acknowledgments

 
This study was funded by the Pacific Vascular Research Foundation. Dr Johnston is supported by NIH NS02042; Dr Browner is supported by NIH NS36016; Dr Dean is supported by NIH EY/AI12219 and AI39499. We thank Jocelyn Phegan and Amy Helmer for excellent technical assistance.

Received July 16, 2001; revision received August 21, 2001; accepted September 5, 2001.

	References

Top Abstract Introduction Subjects and Methods Results Discussion References

 

1. Young B, Gleeson M, Cripps AW. C-reactive protein: a critical review. Pathology. 1991; 23: 118–124.[Medline] [Order article via Infotrieve]
2. Kuller LH, Tracy RP, Shaten J, Meilahn EN. Relation of C-reactive protein and coronary heart disease in the MRFIT nested case-control study: Multiple Risk Factor Intervention Trial. Am J Epidemiol. 1996; 144: 537–547.[Abstract/Free Full Text]
3. Ridker PM, Cushman M, Stampfer MJ, Tracy RP, Hennekens CH. Inflammation, aspirin, and the risk of cardiovascular disease in apparently healthy men. N Engl J Med. 1997; 336: 973–979.[Abstract/Free Full Text]
4. Ridker PM, Buring JE, Shih J, Matias M, Hennekens CH. Prospective study of C-reactive protein and the risk of future cardiovascular events among apparently healthy women. Circulation. 1998; 98: 731–733.[Abstract/Free Full Text]
5. Rader DJ. Inflammatory markers of coronary risk. N Engl J Med. 2000; 343: 1179–1182.[Free Full Text]
6. Ross R. Atherosclerosis: an inflammatory disease. N Engl J Med. 1999; 340: 115–126.[Free Full Text]
7. Munro JM, Cotran RS. The pathogenesis of atherosclerosis: atherogenesis and inflammation. Lab Invest. 1988; 58: 249–261.[Medline] [Order article via Infotrieve]
8. Kohchi K, Takebayashi S, Hiroki T, Nobuyoshi M. Significance of adventitial inflammation of the coronary artery in patients with unstable angina: results at autopsy. Circulation. 1985; 71: 709–716.[Abstract/Free Full Text]
9. Sato T, Takebayashi S, Kohchi K. Increased subendothelial infiltration of the coronary arteries with monocytes/macrophages in patients with unstable angina: histological data on 14 autopsied patients. Atherosclerosis. 1987; 68: 191–197.[Medline] [Order article via Infotrieve]
10. Kuo CC, Grayston JT, Campbell LA, Goo YA, Wissler RW, Benditt EP. Chlamydia pneumoniae (TWAR) in coronary arteries of young adults (15–34 years old). Proc Natl Acad Sci U S A. 1995; 92: 6911–6914.[Abstract/Free Full Text]
11. Grayston JT, Kuo CC, Coulson AS, Campbell LA, Lawrence RD, Lee MJ, Strandness ED, Wang SP. Chlamydia pneumoniae (TWAR) in atherosclerosis of the carotid artery. Circulation. 1995; 92: 3397–3400.[Abstract/Free Full Text]
12. Ramirez JA. Isolation of Chlamydia pneumoniae from the coronary artery of a patient with coronary atherosclerosis: the Chlamydia pneumoniae/Atherosclerosis Study Group. Ann Intern Med. 1996; 125: 979–982.[Abstract/Free Full Text]
13. Maass M, Bartels C, Krüger S, Krause E, Engel PM, Dalhoff K. Endovascular presence of Chlamydia pneumoniae DNA is a generalized phenomenon in atherosclerotic vascular disease. Atherosclerosis. 1998; 140 (suppl 1): S25–S30.
14. Jackson LA, Campbell LA, Kuo CC, Rodriguez DI, Lee A, Grayston JT. Isolation of Chlamydia pneumoniae from a carotid endarterectomy specimen. J Infect Dis. 1997; 176: 292–295.[Medline] [Order article via Infotrieve]
15. LaBiche R, Koziol D, Quinn TC, Gaydos C, Azhar S, Ketron G, Sood S, DeGraba TJ. Presence of Chlamydia pneumoniae in human symptomatic and asymptomatic carotid atherosclerotic plaque. Stroke. 2001; 32: 855–860.[Abstract/Free Full Text]
16. Berger M, Schröder B, Daeschlein G, Schneider W, Busjahn A, Buchwalow I, Luft FC, Haller H. Chlamydia pneumoniae DNA in non-coronary atherosclerotic plaques and circulating leukocytes. J Lab Clin Med. 2000; 136: 194–200.[Medline] [Order article via Infotrieve]
17. Apfalter P, Loidl M, Nadrchal R, Makristathis A, Rotter M, Bergmann M, Polterauer P, Hirschl AM. Isolation and continuous growth of Chlamydia pneumoniae from arterectomy specimens. Eur J Clin Microbiol Infect Dis. 2000; 19: 305–308.[Medline] [Order article via Infotrieve]
18. Maass M, Bartels C, Engel PM, Mamat U, Sievers HH. Endovascular presence of viable Chlamydia pneumoniae is a common phenomenon in coronary artery disease. J Am Coll Cardiol. 1998; 31: 827–832.[Abstract/Free Full Text]
19. Esposito G, Blasi F, Allegra L, Chiesa R, Melissano G, Cosentini R, Tarsia P, Dordoni L, Cantoni C, Arosio C, Fagetti L. Demonstration of viable Chlamydia pneumoniae in atherosclerotic plaques of carotid arteries by reverse transcriptase polymerase chain reaction. Ann Vasc Surg. 1999; 13: 421–425.[Medline] [Order article via Infotrieve]
20. Byrne GI, Kalayoglu MV. Chlamydia pneumoniae and atherosclerosis: links to the disease process. Am Heart J. 1999; 138: S488–S490.[Medline] [Order article via Infotrieve]
21. Danesh J, Collins R, Peto R. Chronic infections and coronary heart disease: is there a link? Lancet. 1997; 350: 430–436.[Medline] [Order article via Infotrieve]
22. Gurfinkel E, Bozovich G. Chlamydia pneumoniae: inflammation and instability of the atherosclerotic plaque. Atherosclerosis. 1998; 140 (suppl 1): S31–S35.
23. Ridker PM. Inflammation, infection, and cardiovascular risk: how good is the clinical evidence? Circulation. 1998; 97: 1671–1674.[Free Full Text]
24. Roivainen M, Viik-Kajander M, Palosuo T, Toivanen P, Leinonen M, Saikku P, Tenkanen L, Manninen V, Hovi T, Mänttäri M. Infections, inflammation, and the risk of coronary heart disease. Circulation. 2000; 101: 252–257.[Abstract/Free Full Text]
25. Toss H, Gnarpe J, Gnarpe H, Siegbahn A, Lindahl B, Wallentin L. Increased fibrinogen levels are associated with persistent Chlamydia pneumoniae infection in unstable coronary artery disease. Eur Heart J. 1998; 19: 570–577.[Abstract/Free Full Text]
26. Zhu J, Quyyumi AA, Norman JE, Csako G, Waclawiw MA, Shearer GM, Epstein SE. Effects of total pathogen burden on coronary artery disease risk and C-reactive protein levels. Am J Cardiol. 2000; 85: 140–146.[Medline] [Order article via Infotrieve]
27. Ridker PM, Hennekens CH, Buring JE, Kundsin R, Shih J. Baseline IgG antibody titers to Chlamydia pneumoniae, Helicobacter pylori, herpes simplex virus, and cytomegalovirus and the risk for cardiovascular disease in women. Ann Intern Med. 1999; 131: 573–577.[Abstract/Free Full Text]
28. Ridker PM, Kundsin RB, Stampfer MJ, Poulin S, Hennekens CH. Prospective study of Chlamydia pneumoniae IgG seropositivity and risks of future myocardial infarction. Circulation. 1999; 99: 1161–1164.[Abstract/Free Full Text]
29. Hoffmeister A, Rothenbacher D, Wanner P, Bode G, Persson K, Brenner H, Hombach V, Koenig W. Seropositivity to chlamydial lipopolysaccharide and Chlamydia pneumoniae, systemic inflammation and stable coronary artery disease: negative results of a case-control study. J Am Coll Cardiol. 2000; 35: 112–118.[Abstract/Free Full Text]
30. Chiu B, Viira E, Tucker W, Fong IW. Chlamydia pneumoniae, cytomegalovirus, and herpes simplex virus in atherosclerosis of the carotid artery. Circulation. 1997; 96: 2144–2148.[Abstract/Free Full Text]
31. Berk BC, Weintraub WS, Alexander RW. Elevation of C-reactive protein in "active" coronary artery disease. Am J Cardiol. 1990; 65: 168–172.[Medline] [Order article via Infotrieve]
32. Dean D, Oudens E, Bolan G, Padian N, Schachter J. Major outer membrane protein variants of Chlamydia trachomatis are associated with severe upper genital tract infections and histopathology in San Francisco. J Infect Dis. 1995; 172: 1013–1022.[Medline] [Order article via Infotrieve]
33. Dean D, Shama A, Schachter J, Dawson CR. Molecular identification of an avian strain of Chlamydia psittaci causing severe keratoconjunctivitis in a bird fancier. Clin Infect Dis. 1995; 20: 1179–1185.[Medline] [Order article via Infotrieve]
34. Vichinsky EP, Neumayr LD, Earles AN, Williams R, Lennette ET, Dean D, Nickerson B, Orringer E, McKie V, Bellevue R, Daeschner C, Manci EA. Causes and outcomes of the acute chest syndrome in sickle cell disease: National Acute Chest Syndrome Study Group. N Engl J Med. 2000; 342: 1855–1865.[Abstract/Free Full Text]
35. Lietman T, Brooks D, Moncada J, Schachter J, Dawson C, Dean D. Chronic follicular conjunctivitis associated with Chlamydia psittaci or Chlamydia pneumoniae. Clin Infect Dis. 1998; 26: 1335–1340.[Medline] [Order article via Infotrieve]
36. Phan R, Pham S, Dean D. Characterization of the immune response in conjunctival scarring due to Chlamydia trachomatis among Vietnamese patients.In: Proceedings of the American Society of Microbiology Annual Meeting; May 4–8, 1997; Miami, Fla; 209.
37. Reiner SL, Zheng S, Corry DB, Locksley RM. Constructing polycompetitor cDNAs for quantitative PCR. J Immunol Methods. 1993; 165: 37–46.[Medline] [Order article via Infotrieve]
38. Rödel J, Woytas M, Groh A, Schmidt KH, Hartmann M, Lehmann M, Straube E. Production of basic fibroblast growth factor and interleukin 6 by human smooth muscle cells following infection with Chlamydia pneumoniae. Infect Immun. 2000; 68: 3635–3641.[Abstract/Free Full Text]
39. Bataille R, Klein B. C-reactive protein levels as a direct indicator of interleukin-6 levels in humans in vivo. Arthritis Rheum. 1992; 35: 982–984.[Medline] [Order article via Infotrieve]
40. Gurfinkel E, Bozovich G, Beck E, Testa E, Livellara B, Mautner B. Treatment with the antibiotic roxithromycin in patients with acute non-Q-wave coronary syndromes: the final report of the ROXIS Study. Eur Heart J. 1999; 20: 121–127.[Abstract/Free Full Text]
41. Muhlestein JB, Anderson JL, Carlquist JF, Salunkhe K, Horne BD, Pearson RR, Bunch TJ, Allen A, Trehan S, Nielson C. Randomized secondary prevention trial of azithromycin in patients with coronary artery disease: primary clinical results of the ACADEMIC study. Circulation. 2000; 102: 1755–1760.[Abstract/Free Full Text]
42. Torgano G, Cosentini R, Mandelli C, Perondi R, Blasi F, Bertinieri G, Tien TV, Ceriani G, Tarsia P, Arosio C, Ranzi ML Treatment of Helicobacter pylori and Chlamydia pneumoniae infections decreases fibrinogen plasma level in patients with ischemic heart disease. Circulation. 1999; 99: 1555–1559.[Abstract/Free Full Text]

This article has been cited by other articles:

				A. G. Joyee, H. Qiu, S. Wang, Y. Fan, L. Bilenki, and X. Yang Distinct NKT Cell Subsets Are Induced by Different Chlamydia Species Leading to Differential Adaptive Immunity and Host Resistance to the Infections J. Immunol., January 15, 2007; 178(2): 1048 - 1058. [Abstract] [Full Text] [PDF]

				J.E. den Hartog, S.A. Morre, and J.A. Land Chlamydia trachomatis-associated tubal factor subfertility: immunogenetic aspects and serological screening Hum. Reprod. Update, November 1, 2006; 12(6): 719 - 730. [Abstract] [Full Text] [PDF]

				T W Weiss, H Kvakan, C Kaun, M Prager, W S Speidl, G Zorn, S Pfaffenberger, I Huk, G Maurer, K Huber, et al. No evidence for a direct role of Helicobacter pylori and Mycoplasma pneumoniae in carotid artery atherosclerosis J. Clin. Pathol., November 1, 2006; 59(11): 1186 - 1190. [Abstract] [Full Text] [PDF]

				S. C. Johnston, H. Zhang, L. M. Messina, M. T. Lawton, and D. Dean Chlamydia pneumoniae Burden in Carotid Arteries Is Associated With Upregulation of Plaque Interleukin-6 and Elevated C-Reactive Protein in Serum Arterioscler. Thromb. Vasc. Biol., December 1, 2005; 25(12): 2648 - 2653. [Abstract] [Full Text] [PDF]

				J.E. den Hartog, J.A. Land, F.R.M. Stassen, A.G.H. Kessels, and C.A. Bruggeman Serological markers of persistent C. trachomatis infections in women with tubal factor subfertility Hum. Reprod., April 1, 2005; 20(4): 986 - 990. [Abstract] [Full Text] [PDF]

				P. J. Lindsberg and A. J. Grau Inflammation and Infections as Risk Factors for Ischemic Stroke Stroke, October 1, 2003; 34(10): 2518 - 2532. [Abstract] [Full Text] [PDF]

				K. Yaffe, K. Lindquist, B. W. Penninx, E. M. Simonsick, M. Pahor, S. Kritchevsky, L. Launer, L. Kuller, S. Rubin, and T. Harris Inflammatory markers and cognition in well-functioning African-American and white elders Neurology, July 8, 2003; 61(1): 76 - 80. [Abstract] [Full Text] [PDF]

				P. Khairy, S. Rinfret, J.-C. Tardif, R. Marchand, S. Shapiro, J. Brophy, and J. Dupuis Absence of Association Between Infectious Agents and Endothelial Function in Healthy Young Men Circulation, April 22, 2003; 107(15): 1966 - 1971. [Abstract] [Full Text] [PDF]

				M. Prager, Z. Turel, W. S. Speidl, G. Zorn, C. Kaun, A. Niessner, G. Heinze, I. Huk, G. Maurer, K. Huber, et al. Chlamydia pneumoniae in Carotid Artery Atherosclerosis: A Comparison of Its Presence in Atherosclerotic Plaque, Healthy Vessels, and Circulating Leukocytes From the Same Individuals Stroke, December 1, 2002; 33(12): 2756 - 2761. [Abstract] [Full Text] [PDF]

				D. Sander, K. Winbeck, J. Klingelhofer, T. Etgen, and B. Conrad Reduced Progression of Early Carotid Atherosclerosis After Antibiotic Treatment and Chlamydia pneumoniae Seropositivity Circulation, November 5, 2002; 106(19): 2428 - 2433. [Abstract] [Full Text] [PDF]

This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Johnston, S. C. Articles by Dean, D. Search for Related Content PubMed PubMed Citation Articles by Johnston, S. C. Articles by Dean, D. Pubmed/NCBI databases Substance via MeSH Medline Plus Health Information Carotid Artery Disease Related Collections Mechanism of atherosclerosis/growth factors Pathophysiology Risk Factors Carotid Stenosis Risk Factors for Stroke