Donate Help Contact The AHA Sign In Home
American Heart Association
Stroke
Search: search_blue_button Advanced Search
Stroke. 2004;35:1506-1518
Published online before print April 29, 2004, doi: 10.1161/01.STR.0000126891.93919.4e
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
35/6/1506    most recent
01.STR.0000126891.93919.4ev1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wojcik, C.
Right arrow Articles by Di Napoli, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wojcik, C.
Right arrow Articles by Di Napoli, M.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
Medline Plus Health Information
*Stroke
Related Collections
Right arrow Cell biology/structural biology
Right arrow Cell signalling/signal transduction
Right arrow Acute Cerebral Infarction
Right arrow Pathology of Stroke
Right arrow Other Stroke Treatment - Medical

(Stroke. 2004;35:1506.)
© 2004 American Heart Association, Inc.


Progress Review

Ubiquitin-Proteasome System and Proteasome Inhibition: New Strategies in Stroke Therapy

Cezary Wojcik, MD, PhD, DSc Mario Di Napoli, MD

From the Department of Physiology (C.W.), University of Texas Southwestern Medical Center, Dallas, TX; and Neurological Section (M.D.N.), SMDN–Center for Cardiovascular Medicine and Cerebrovascular Disease Prevention, Sulmona (L’Aquila), Italy.

Correspondence to Dr Mario Di Napoli, Neurological Section SMDN–Center for Cardiovascular Medicine and Cerebrovascular Disease Prevention, Via Trento, 41 67039, Sulmona (AQ), Italy. E-mail mariodinapoli{at}katamail.com


*    Abstract
up arrowTop
*Abstract
down arrowIntroduction
down arrowUbiquitin and Ubiquitination
down arrow20S and 26S Proteasomes
down arrowLocalization of Proteasomes...
down arrowPhysiological Roles of...
down arrowRole of the UPS...
down arrowUPS in the Central...
down arrowGeneral Properties and Chemistry...
down arrowChanges in the UPS...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of UPS...
down arrowUPS and Mitochondrial Function
down arrowAdjusted Changes of UPS...
down arrowRelationship of UPS and...
down arrowUPS and Protein Synthesis
down arrowUPS and the Damage...
down arrowUPS and Protein Kinases
down arrowUPS and Gene-Mediated Effects...
down arrowUPS and Heat Shock...
down arrowUPS and Ischemic Core...
down arrowProteasome Inhibitors in Animal...
down arrowTime-Dependent and Cell...
down arrowLimitations, Future Areas, and...
down arrowReferences
 
Background and Purpose— Proteasomes are large multicatalytic proteinase complexes that are found in the cytosol and in the nucleus of eukaryotic cells with a central role in cellular protein turnover. The ubiquitin-proteasome system (UPS) has a central role in the selective degradation of intracellular proteins. Among the key proteins whose levels are modulated by the proteasome are those involved in the control of inflammatory processes, cell cycle regulation, and gene expression. There are now overwhelming data suggesting that the UPS contributes to cerebral ischemic injury.

Summary of Review— Proteasome inhibition is a potential treatment option for stroke. Thus far, proof of principle has been obtained from studies in several animal models of cerebral ischemia. Treatment with proteasome inhibitors reduces effectively neuronal and astrocytic degeneration, cortical infarct volume, infarct neutrophil infiltration, and NF-{kappa}B immunoreactivity with an extension of the neuroprotective effect at least 6 hours after ischemic insult. However, it is clear that the UPS represents a central pathway for the processing and metabolism of multiple proteins with critical roles in cellular function. To avoid eliciting significant side effects associated with complete inhibition of the proteasome and the possible immunosuppressive effects from persistent suppression of NF-{kappa}B activation, it is critical that we understand how to partially and temporally attenuate proteasome function to elicit the desired therapeutic effect before any large-scale use in humans.

Conclusion— This review highlights the most recent advances in our knowledge on UPS, as well as the early experience of using proteasome inhibition strategies to treat acute stroke.


Key Words: cerebral ischemia • NF-kappa B • inflammation • ubiquitinproteasome


*    Introduction
up arrowTop
up arrowAbstract
*Introduction
down arrowUbiquitin and Ubiquitination
down arrow20S and 26S Proteasomes
down arrowLocalization of Proteasomes...
down arrowPhysiological Roles of...
down arrowRole of the UPS...
down arrowUPS in the Central...
down arrowGeneral Properties and Chemistry...
down arrowChanges in the UPS...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of UPS...
down arrowUPS and Mitochondrial Function
down arrowAdjusted Changes of UPS...
down arrowRelationship of UPS and...
down arrowUPS and Protein Synthesis
down arrowUPS and the Damage...
down arrowUPS and Protein Kinases
down arrowUPS and Gene-Mediated Effects...
down arrowUPS and Heat Shock...
down arrowUPS and Ischemic Core...
down arrowProteasome Inhibitors in Animal...
down arrowTime-Dependent and Cell...
down arrowLimitations, Future Areas, and...
down arrowReferences
 
The progression and extension of cerebral ischemia are related to several mechanisms, many of which involve an inflammatory response component.1 Recent neuroprotective strategies include targeting inflammatory mediators as a whole, although it has proved difficult because of their redundancy. Alternate inflammatory pathways may circumvent the suppression of a single targeted mediator.2 Targeting inflammatory cascades as a whole is another approach, although it has shown no benefit.3 Novel approaches have now focused on alteration of inflammatory transcriptional factors to simultaneously interfere with the upregulation of multiple inflammatory genes.

One promising approach is to suppress the activation of transcription nuclear factor-kappa B (NF-{kappa}B) by stabilizing the inhibitory protein I{kappa}B via inhibition of the ubiquitin (Ub)-proteasome system (UPS).4 UPS is the major nonlysosomal pathway of proteolysis in human cells and accounts for the degradation of most short-lived, misfolded, or damaged proteins, as well as long-lived proteins. This pathway is important in the regulation of a number of key biological regulatory mechanisms.4,5 Inhibitors that target the UPS should suppress the activation of NF-{kappa}B by stabilizing I{kappa}B, thereby reducing levels of multiple proinflammatory proteins, providing antiinflammatory effects and, ultimately, successfully attenuating the inflammatory cascade in cerebral ischemia, leading to the reduction of the ischemic damage. As such, proteasome inhibitors are a novel approach to the treatment of cerebral ischemia. They have entered clinical evaluation based on efficacy and action demonstrated in laboratory studies, uncovering insights into the pathophysiology of cerebral ischemia.


*    Ubiquitin and Ubiquitination
up arrowTop
up arrowAbstract
up arrowIntroduction
*Ubiquitin and Ubiquitination
down arrow20S and 26S Proteasomes
down arrowLocalization of Proteasomes...
down arrowPhysiological Roles of...
down arrowRole of the UPS...
down arrowUPS in the Central...
down arrowGeneral Properties and Chemistry...
down arrowChanges in the UPS...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of UPS...
down arrowUPS and Mitochondrial Function
down arrowAdjusted Changes of UPS...
down arrowRelationship of UPS and...
down arrowUPS and Protein Synthesis
down arrowUPS and the Damage...
down arrowUPS and Protein Kinases
down arrowUPS and Gene-Mediated Effects...
down arrowUPS and Heat Shock...
down arrowUPS and Ischemic Core...
down arrowProteasome Inhibitors in Animal...
down arrowTime-Dependent and Cell...
down arrowLimitations, Future Areas, and...
down arrowReferences
 
Ub is covalently attached to other proteins via either an isopeptide (via {epsilon}-amino groups of internal Lys) or peptide bond.5 Ub conjugation (ubiquitination) is performed by 1 of multiple Ub ligases (E3s). E3s transfer Ub from 1 of several Ub-conjugating enzymes (UBCs or E2s), which obtain Ub from the Ub-activating enzyme (UBA or E1). Ub activation requires energy provided by ATP hydrolysis (Figure 1). The recognition of a substrate by an E3 involves various mechanisms: the presence of a specific primary sequence, an N-terminal destabilizing amino acid, phosphorylation of a specific residue, or poorly characterized structural motifs.5 Polyubiquitin chains are often trimmed by multiple deubiquitinating enzymes (DUBs), which counteract the E1-E2-E3 cascade rescuing certain substrates from degradation.6



View larger version (28K):
[in this window]
[in a new window]
 
Figure 1. The ubiquitin- and proteasome-dependent system of protein degradation. The ubiquitin-activating enzyme (E1) forms a thioester intermediate with ubiquitin (Ub), transferring it to 1 of the Ub conjugating enzymes (E2). E2 interacts with the Ub ligases (E3), which recognize different substrates to be ubiquinated. Ub moieties are then transferred to the substrates, forming poly-Ub chains. Lys-48–linked poly-Ub chains are recognized and bound by the 26S proteasomes. Finally, the substrates are degraded into peptides while free Ub is recycled. Monoubiquitinated proteins and chains with other than Lys-48 linkages serve nonproteolytical functions. Ubiquitinated substrates can be deubiquitinated by the action of 1 of several deubiquitinating enzymes (DUBs). 26S proteasome is composed of the 20S proteasome and 2 PA700 caps. 20S proteasome by itself is able to degrade unfolded and oxidized proteins. It can also associate with different activators, such as PA28.


*    20S and 26S Proteasomes
up arrowTop
up arrowAbstract
up arrowIntroduction
up arrowUbiquitin and Ubiquitination
*20S and 26S Proteasomes
down arrowLocalization of Proteasomes...
down arrowPhysiological Roles of...
down arrowRole of the UPS...
down arrowUPS in the Central...
down arrowGeneral Properties and Chemistry...
down arrowChanges in the UPS...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of UPS...
down arrowUPS and Mitochondrial Function
down arrowAdjusted Changes of UPS...
down arrowRelationship of UPS and...
down arrowUPS and Protein Synthesis
down arrowUPS and the Damage...
down arrowUPS and Protein Kinases
down arrowUPS and Gene-Mediated Effects...
down arrowUPS and Heat Shock...
down arrowUPS and Ischemic Core...
down arrowProteasome Inhibitors in Animal...
down arrowTime-Dependent and Cell...
down arrowLimitations, Future Areas, and...
down arrowReferences
 
The 26S proteasomes are composed of the 20S proteasome core and two 19S caps (PA700 activators). The 20S proteasome is a barrel-shaped molecule made up of 4 stacked rings, 2 outer {alpha}-rings and 2 inner ß-rings. Each ring is made up of 7 different subunits of the {alpha}-type and ß-type, respectively. Cleavage of the peptide bonds is performed in the central catalytic chamber of the 20S proteasome by a nucleophile attack of an N-terminal Thr residue of either ß5, ß2, or ß1 subunits,6,7 displaying different substrate specificity. These activities have been named chymotrypsin-like (ChTL), trypsin-like (TL), and post-glutamyl-peptide hydrolyzing (PGPH), because they cleave peptides after hydrophobic, basic, and acidic residues, respectively.8

20S proteasomes degrade short peptides and unfolded proteins. Unfolded polypeptide chains reach the central chamber by 1 of the termini or in the form of a loop.9 PA700 has an affinity for the poly-Ub chains, bringing ubiquitinated substrates to the proteasome. Because of its "unfoldase" or "inverse chaperone" activity, the PA700 allows the degradation of folded proteins.6,5 Although the target protein itself is unfolded and fed within the central chamber of the proteasome, the poly-Ub chain is removed and disassembled.10

PA700 contains a hexameric ring of AAA ATPases binding the proteasomal {alpha}-rings: it forms the "base" together with 2 other subunits, while the remaining 9 PA700 subunits form its "lid."11,12 The 26S enzyme has a central role in the UPS; however, the 20S core enzyme may play an important role in the degradation of unfolded proteins. Such proteins become more abundant within the cells in situations of oxidative stress. Although ATP depletion associated with ischemia impairs the 26S proteolytic activity, the 20S proteasome remains active.13,14


*    Localization of Proteasomes Within the Cells
up arrowTop
up arrowAbstract
up arrowIntroduction
up arrowUbiquitin and Ubiquitination
up arrow20S and 26S Proteasomes
*Localization of Proteasomes...
down arrowPhysiological Roles of...
down arrowRole of the UPS...
down arrowUPS in the Central...
down arrowGeneral Properties and Chemistry...
down arrowChanges in the UPS...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of UPS...
down arrowUPS and Mitochondrial Function
down arrowAdjusted Changes of UPS...
down arrowRelationship of UPS and...
down arrowUPS and Protein Synthesis
down arrowUPS and the Damage...
down arrowUPS and Protein Kinases
down arrowUPS and Gene-Mediated Effects...
down arrowUPS and Heat Shock...
down arrowUPS and Ischemic Core...
down arrowProteasome Inhibitors in Animal...
down arrowTime-Dependent and Cell...
down arrowLimitations, Future Areas, and...
down arrowReferences
 
Proteasomes are present within the nucleus and cytoplasm of all cells, making up to 1% of total cell protein.15 Most proteasomes diffuse freely within cytoplasm and nucleoplasm; however, a fraction is stably associated with different structures. Proteasomes are enriched at discrete subnuclear foci called the PML bodies, whereas in the cytoplasm, proteasome subpopulations are associated with the external surface of the endoplasmic reticulum (ER) and the cytoskeleton16 and the centrosome.17 In situations of impaired UPS function, ubiquitinated proteins accumulate at PML bodies and around the centrosome, forming a single aggregate,18,19 or "aggresome." Formation of such aggregates is achieved in vitro by the action of proteasome inhibitors and by overexpression of certain proteins usually degraded by the UPS.


*    Physiological Roles of Proteasomes
up arrowTop
up arrowAbstract
up arrowIntroduction
up arrowUbiquitin and Ubiquitination
up arrow20S and 26S Proteasomes
up arrowLocalization of Proteasomes...
*Physiological Roles of...
down arrowRole of the UPS...
down arrowUPS in the Central...
down arrowGeneral Properties and Chemistry...
down arrowChanges in the UPS...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of UPS...
down arrowUPS and Mitochondrial Function
down arrowAdjusted Changes of UPS...
down arrowRelationship of UPS and...
down arrowUPS and Protein Synthesis
down arrowUPS and the Damage...
down arrowUPS and Protein Kinases
down arrowUPS and Gene-Mediated Effects...
down arrowUPS and Heat Shock...
down arrowUPS and Ischemic Core...
down arrowProteasome Inhibitors in Animal...
down arrowTime-Dependent and Cell...
down arrowLimitations, Future Areas, and...
down arrowReferences
 
In yeast, the 20S proteasome subunits and several PA700 subunits are essential.20,21 Block in the cell cycle and induction of apoptosis are obvious effects of proteasome inhibition resulting from the inhibition of the degradation of regulatory proteins.22 Cells treated with proteasome inhibitors accumulate numerous polyubiquitinated proteins. Regulatory proteins are only a fraction of them, most of them being made up of aged structural and functional proteins, which are normally turned over by the UPS. Many polyubiquitinated proteins are the defective newly translated proteins.23 Aggregates of heavily polyubiquitinated proteins may be engulfed by autophagosomes and degraded by the lysosomal pathway, therefore rescuing cells from a death by suffocation with unwanted proteins. It is a matter of discussion whether the formation of protein aggregates is toxic or beneficial in serving to sequester ubiquitinated proteins from wreaking havoc within the cell.24

Peptides generated by proteasomes have from few to 20–30 amino acids. They are further degraded by cytosolic peptidases; however, a fraction of them are delivered to ER-associated TAP peptide transporter. Once in the ER lumen, they bind the assembling MHC class I molecules. Mature MHC class I molecules are exported to the cell surface, where they present the antigenic peptides to immunologically competent cells.25,26


*    Role of the UPS in the Inflammatory Pathways: I{kappa}B{alpha} Signaling
up arrowTop
up arrowAbstract
up arrowIntroduction
up arrowUbiquitin and Ubiquitination
up arrow20S and 26S Proteasomes
up arrowLocalization of Proteasomes...
up arrowPhysiological Roles of...
*Role of the UPS...
down arrowUPS in the Central...
down arrowGeneral Properties and Chemistry...
down arrowChanges in the UPS...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of UPS...
down arrowUPS and Mitochondrial Function
down arrowAdjusted Changes of UPS...
down arrowRelationship of UPS and...
down arrowUPS and Protein Synthesis
down arrowUPS and the Damage...
down arrowUPS and Protein Kinases
down arrowUPS and Gene-Mediated Effects...
down arrowUPS and Heat Shock...
down arrowUPS and Ischemic Core...
down arrowProteasome Inhibitors in Animal...
down arrowTime-Dependent and Cell...
down arrowLimitations, Future Areas, and...
down arrowReferences
 
Inflammation can be defined as a complex set of interactions among soluble factors and cells that arise in any tissue in response to traumatic, infectious, postischemic, toxic, or autoimmune injury.27 The inflammatory process normally leads to recovery and healing; however, it can often lead to persistent tissue damage, caused by the infiltration and activity of inflammatory cells. An inflammatory response is also present in cerebral ischemia28 and after an acute ischemic stroke as sustained and persistent inflammatory response.29–31

Inflammatory pathways are regulated by a limited number of transcription factors, the most important being NF-{kappa}B. NF-{kappa}B is a collective name for dimeric transcription factors of the Rel family. Its most abundant form is the cytoplasmic p65/p50 dimer, bound to I{kappa}B{alpha}.32 On stimulation of various cell types by several cytokines (IL-1, TNF-{alpha}), bacterial lipopolysaccharide, UV radiation, ionizing radiation, or oxidative stress, a signal transduction cascade is activated, leading to the phosphorylation of I{kappa}B{alpha} on Ser 32 and 36 by the multimeric IKK (I{kappa}B kinase) complex.

IKK-mediated phosphorylation triggers the ubiquitination of I{kappa}B{alpha} by the E3 ligase SCFßTRCP. Ubiquitinated I{kappa}B{alpha} is targeted to the 26S proteasome.33 Once I{kappa}B{alpha} is degraded, the nuclear localization signal of NF-{kappa}B is unmasked, allowing its translocation to the nucleus where it binds to promoter regions of several proinflammatory genes, inducing their expression and thus amplifying the inflammatory response (Figure 2).



View larger version (49K):
[in this window]
[in a new window]
 
Figure 2. Role of the ubiquitin- and proteasome-dependent system of protein degradation (UPS) in NF-{kappa}B signaling during an inflammatory reaction. On extracellular signals (TNF-{alpha}, IL-2) or insults such as reactive oxygen species (ROS), a signaling cascade leads to the formation of Lys-63–linked chains on TRAF6, which mediates activation of IKK kinase. IKK phosphorylates I{kappa}B{alpha} bound to the p65/p50 NF-{kappa}B dimer in the cytoplasm. Phosphorylated NF-{kappa}B is ubiquitinated by the SCFßTRCP E3 complex and degraded by the 26S proteasome, releasing the p65/p50 dimer. The latter immediately translocates to the nucleus where it binds to specific promoter sequences initiating transcription of NF-{kappa}B-dependent genes, many of them mediators of the inflammatory response. p50 itself is generated from a cytoplasmic p105 precursor by a unique mechanism involving partial proteolysis mediated by the 26S proteasome.

The p50 subunit of NF-{kappa}B is generated from the p105 precursor by limited proteolytic cleavage mediated by the 26S proteasome.34,35 The intensity of NF-{kappa}B activation depends on various factors, including the variable E3 activity of the SCFßTRCP complex, which is regulated by a reversible covalent modification with the Ub-like protein NEDD8.36 Finally, the activity of the IKK kinase depends on the formation of unusual poly-Ub chains linked by Lys63.37


*    UPS in the Central Nervous System
up arrowTop
up arrowAbstract
up arrowIntroduction
up arrowUbiquitin and Ubiquitination
up arrow20S and 26S Proteasomes
up arrowLocalization of Proteasomes...
up arrowPhysiological Roles of...
up arrowRole of the UPS...
*UPS in the Central...
down arrowGeneral Properties and Chemistry...
down arrowChanges in the UPS...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of UPS...
down arrowUPS and Mitochondrial Function
down arrowAdjusted Changes of UPS...
down arrowRelationship of UPS and...
down arrowUPS and Protein Synthesis
down arrowUPS and the Damage...
down arrowUPS and Protein Kinases
down arrowUPS and Gene-Mediated Effects...
down arrowUPS and Heat Shock...
down arrowUPS and Ischemic Core...
down arrowProteasome Inhibitors in Animal...
down arrowTime-Dependent and Cell...
down arrowLimitations, Future Areas, and...
down arrowReferences
 
UPS components are among the most abundant proteins of the central nervous system (CNS), but the relative levels of individual components show high variability between different regions and cell types.38,39 Ub immunoreactivity is normally diffuse; however, in various pathological conditions, it concentrates in neuronal inclusion bodies, suggesting that neurodegenerative disorders can involve an impairment of the UPS function.38

Neuronal differentiation in vitro is accompanied by increased levels of Ub conjugates, decreased levels of free Ub, enhanced capacity of ubiquitination, increased proteasome activity, and induction of immunoproteasome subunits.38,40–42 Intense Ub immunoreactivity correlates with neuronal differentiation, involving dendrite outgrowth and arborization in vivo.43,44 Ub is also involved in the sodium-dependent uptake of various neurotransmitters in the cerebral cortex.45 Curiously, while dipeptide inhibitors of the N-end rule prevent neurite outgrowth, proteasome inhibitors induce neurite outgrowth,41,46 indicating that UPS role in the CNS goes far beyond a simple degradative role. At higher doses and longer expositions, proteasome inhibitors induce neuronal cell death.47

The C-terminal Ub hydrolase UCH-L1 (PGP 9.5) constitutes 1% to 2% of total soluble brain protein and is used as a marker of neurons and neuroendocrine cells. UPS activity is required for experience-dependent remodeling of the postsynaptic densities in cultured rat hippocampal neurons.48 UPS at the synapse appears to operate at the presynaptic and at the postsynaptic level. The net outcome of inhibiting the UPS is to enhance synaptic transmission.49

Brain proteasomes display the same basic enzymatic activities.50 They have been detected in the cytoplasm, nuclei, dendrites, axons, and synaptic buttons of various CNS cell types, including pyramidal cells, granular cells of the hippocampus, Purkinje cells, and glial cells.51–53 Activity and expression of brain proteasomes decrease in neurodegenerative disorders and with age, contributing to the elevations in protein oxidation, protein aggregation, and neurodegeneration evident in the aging CNS.54,55 Alterations of ubiquitination of specific substrates caused by mutations of appropriate E3s are often associated with different neurological diseases.56–58


*    General Properties and Chemistry of Proteasome Inhibitors
up arrowTop
up arrowAbstract
up arrowIntroduction
up arrowUbiquitin and Ubiquitination
up arrow20S and 26S Proteasomes
up arrowLocalization of Proteasomes...
up arrowPhysiological Roles of...
up arrowRole of the UPS...
up arrowUPS in the Central...
*General Properties and Chemistry...
down arrowChanges in the UPS...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of UPS...
down arrowUPS and Mitochondrial Function
down arrowAdjusted Changes of UPS...
down arrowRelationship of UPS and...
down arrowUPS and Protein Synthesis
down arrowUPS and the Damage...
down arrowUPS and Protein Kinases
down arrowUPS and Gene-Mediated Effects...
down arrowUPS and Heat Shock...
down arrowUPS and Ischemic Core...
down arrowProteasome Inhibitors in Animal...
down arrowTime-Dependent and Cell...
down arrowLimitations, Future Areas, and...
down arrowReferences
 
Several natural and synthetic compounds that act as proteasome inhibitors have been reported.59 Their chemical classes, chemical structures, and mechanisms of action are summarized in Table 1. One of these compounds, the MLN-519 a synthetic analog of the bacterial metabolite lactacystin,60 is under clinical evaluation for inhibiting reperfusion injury after ischemic CNS injury.61 Other natural proteasome inhibitors include eponemycin,62 epoxomycin,63 aclacinomycin A64 and PR-39, an Arg- and Pro-rich porcine polypeptide.65 Additional synthetic proteasome inhibitors are aldehyde derivatives (CEP-161267,25 or MG13266), dipeptide benzamide derivatives (CVT-63467), and dipeptide boronic acid substitutes (Bortezomib,68 NVP-AFB340, and NVP-AFD31469). Another group of vinyl sulfone tripeptide proteasome inhibitors has been described by Bogyo et al.70 Finally, the HIV-1 protease inhibitor, ritonavir, is also a competitive micromolar inhibitor of the proteasome.71


View this table:
[in this window]
[in a new window]
 
TABLE 1. Representative Classes of Proteasome Inhibitors


*    Changes in the UPS During Ischemia/Reperfusion Injury in the Brain
up arrowTop
up arrowAbstract
up arrowIntroduction
up arrowUbiquitin and Ubiquitination
up arrow20S and 26S Proteasomes
up arrowLocalization of Proteasomes...
up arrowPhysiological Roles of...
up arrowRole of the UPS...
up arrowUPS in the Central...
up arrowGeneral Properties and Chemistry...
*Changes in the UPS...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of UPS...
down arrowUPS and Mitochondrial Function
down arrowAdjusted Changes of UPS...
down arrowRelationship of UPS and...
down arrowUPS and Protein Synthesis
down arrowUPS and the Damage...
down arrowUPS and Protein Kinases
down arrowUPS and Gene-Mediated Effects...
down arrowUPS and Heat Shock...
down arrowUPS and Ischemic Core...
down arrowProteasome Inhibitors in Animal...
down arrowTime-Dependent and Cell...
down arrowLimitations, Future Areas, and...
down arrowReferences
 
The UPS plays a complex and unambiguous role in the etiopathology of cerebral ischemia/reperfusion, both directly and indirectly, because of its pivot role in many intracellular pathways.59


*    Adjusted Changes of Ubiquitin-Proteasome System to Hypoxia
up arrowTop
up arrowAbstract
up arrowIntroduction
up arrowUbiquitin and Ubiquitination
up arrow20S and 26S Proteasomes
up arrowLocalization of Proteasomes...
up arrowPhysiological Roles of...
up arrowRole of the UPS...
up arrowUPS in the Central...
up arrowGeneral Properties and Chemistry...
up arrowChanges in the UPS...
*Adjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of UPS...
down arrowUPS and Mitochondrial Function
down arrowAdjusted Changes of UPS...
down arrowRelationship of UPS and...
down arrowUPS and Protein Synthesis
down arrowUPS and the Damage...
down arrowUPS and Protein Kinases
down arrowUPS and Gene-Mediated Effects...
down arrowUPS and Heat Shock...
down arrowUPS and Ischemic Core...
down arrowProteasome Inhibitors in Animal...
down arrowTime-Dependent and Cell...
down arrowLimitations, Future Areas, and...
down arrowReferences
 
Although brief and acute hypoxia does not impair proteasome function,72 a clear inhibitory effect of hypoxia on proteasome function is evident after prolonged hypoxic periods73 and in the presence of inflammatory mediators.74,75 Repeated and intermittent episodes of hypoxia decrease markedly proteasomal activity in aged Sprague-Dawley rat brain.76 Hypoxia stabilizes the HIF-1{alpha} component of the dimeric hypoxia inducible factor (HIF) transcription factor. Under normoxic conditions, HIF-1{alpha} is ubiquitinated by the Von Hippel-Lindau (VHL) E3 and degraded by the proteasome, whereas the other HIF subunit—HIF-1ß/ARNT (aryl hydrocarbon receptor nuclear translocator)—is constitutively expressed.73 HIF-1{alpha} coordinates the response to prolonged hypoxia, which pertains to glycolysis, glucose transport, vasodilation, and angiogenesis.73 Proteasome inhibitors prevent HIF-1{alpha} degradation,77,78 resulting in accelerated angiogenesis in vitro.79 This mechanism could contribute to the rescue of the penumbra of an ischemic lesion.


*    Adjusted Changes of Ubiquitin-Proteasome System to Ischemia
up arrowTop
up arrowAbstract
up arrowIntroduction
up arrowUbiquitin and Ubiquitination
up arrow20S and 26S Proteasomes
up arrowLocalization of Proteasomes...
up arrowPhysiological Roles of...
up arrowRole of the UPS...
up arrowUPS in the Central...
up arrowGeneral Properties and Chemistry...
up arrowChanges in the UPS...
up arrowAdjusted Changes of Ubiquitin...
*Adjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of UPS...
down arrowUPS and Mitochondrial Function
down arrowAdjusted Changes of UPS...
down arrowRelationship of UPS and...
down arrowUPS and Protein Synthesis
down arrowUPS and the Damage...
down arrowUPS and Protein Kinases
down arrowUPS and Gene-Mediated Effects...
down arrowUPS and Heat Shock...
down arrowUPS and Ischemic Core...
down arrowProteasome Inhibitors in Animal...
down arrowTime-Dependent and Cell...
down arrowLimitations, Future Areas, and...
down arrowReferences
 
In the postischemic hippocampus, conjugated Ub accumulates and free Ub is depleted.72,80,81 The accumulation of conjugated Ub may reflect hypofunction of downstream proteasome activity that normally degrades ubiquitinated proteins. Moreover, direct injection of a proteasome inhibitor into the lateral ventricles of the rat-induced DNA fragmentation in various CNS areas, suggesting that suppression of proteasome is able to induce neuronal apoptosis.82 Therefore, it is reasonable to speculate that proteasome malfunction may in part underlie the molecular events of the ischemia-induced neuronal death. Decreased proteasome activity at the ischemic core and the surrounding tissues allows accumulation of oxidized proteins, resulting in formation of protein aggregates, ER stress, impairment of cell function, and eventually cell death. In an experimental ischemia of rat brains, a 60% elevation of Ub conjugate levels in the ischemic compared with the nonischemic animals was observed within 1 hour of recovery. The conjugate immunoreactivity remained at this level for 6 hours but eventually decreased to control levels by 24 hours of recovery.72,80 Increased formation of poly-Ub conjugates was accompanied with a significant increase in the transcription levels of poly-Ub genes.83


*    Adjusted Changes of Ubiquitin-Proteasome System to ATP Depletion
up arrowTop
up arrowAbstract
up arrowIntroduction
up arrowUbiquitin and Ubiquitination
up arrow20S and 26S Proteasomes
up arrowLocalization of Proteasomes...
up arrowPhysiological Roles of...
up arrowRole of the UPS...
up arrowUPS in the Central...
up arrowGeneral Properties and Chemistry...
up arrowChanges in the UPS...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
*Adjusted Changes of Ubiquitin...
down arrowAdjusted Changes of Ubiquitin...
down arrowAdjusted Changes of UPS...
down arrowUPS and Mitochondrial Function
down arrowAdjusted Changes of UPS...
down arrowRelationship of UPS and...
down arrowUPS and Protein Synthesis
down arrowUPS and the Damage...
down arrowUPS and Protein Kinases
down arrowUPS and Gene-Mediated Effects...
down arrowUPS and Heat Shock...
down arrowUPS and Ischemic Core...
down arrowProteasome Inhibitors in Animal...
down arrowTime-Dependent and Cell...
down arrowLimitations, Future Areas, and...
down arrowReferences
 
Ischemic ATP depletion affects the ubiquitination cascade itself.84–86 One-hour transient focal cerebral ischemia induces marked depletion of the E3 parkin protein levels but does not affect the levels of several E2s. Upregulation of the expression of parkin protects cells from injury induced by ER stress; therefore, parkin depletion may increase the sensitivity of neurons to ER stress and the aggregation of ubiquitinated proteins during the reperfusion period.87 At the ischemic core, the ATP-dependent and Ub-dependent degradation mediated by the 26S proteasome is impaired, whereas the ATP-independent and Ub-independent degradation mediated by 20S proteasome proceeds without obstacles. Many 26S proteasomes dissociate under these conditions into 20S proteasomes and PA700 caps. After ischemia in the gerbil cortex, the 26S proteasome ChTL activity decreases, whereas the 20S proteasome ChTL activity increases.88 Moreover, while in most regions the 26S proteasome activity is recovered after reperfusion, in certain regions (eg, the CA1 region of the hippocampus) PA700 and 20S proteasomes are not fully able to reassociate, indicating the occurrence of irreversible biochemical changes. This probably underlies the delayed neuronal cell death in such regions, which has many features common with neurodegeneration.86


*    Adjusted Changes of Ubiquitin-Proteasome System to Intracellular pH Levels
up arrowTop
up arrowAbstract
up arrowIntroduction
up arrowUbiquitin and Ubiquitination
up arrow20S and 26S Proteasomes
up arrowLocalization of Proteasomes...
up arrowPhysiological Roles of...
up arrowRole of the UPS...
up arrowUPS in the Central...
up arrowGeneral Properties and Chemistry...
up arrowChanges in the UPS...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
*Adjusted Changes of Ubiquitin...
down arrowAdjusted Changes of UPS...
down arrowUPS and Mitochondrial Function
down arrowAdjusted Changes of UPS...
down arrowRelationship of UPS and...
down arrowUPS and Protein Synthesis
down arrowUPS and the Damage...
down arrowUPS and Protein Kinases
down arrowUPS and Gene-Mediated Effects...
down arrowUPS and Heat Shock...
down arrowUPS and Ischemic Core...
down arrowProteasome Inhibitors in Animal...
down arrowTime-Dependent and Cell...
down arrowLimitations, Future Areas, and...
down arrowReferences
 
The cellular site of action of low pH is not completely resolved. Effects on UPS that appear to exist but have not yet been extensively investigated appear the most likely and are quite reasonable. Proteasome inhibition is correlated with hypoxia-evoked decreases in extracellular and intracellular pH.74 Certainly, less specifically, pH may act by altering proteasome subunits at critical times, by direct transient denaturation or indirectly by enhancement of free radical formation (via iron delocalization and the Fenton reaction),89 or more specifically by altering subunit displacement of the proteosomal complex catalytic activity and altering the action of Ub-protein-ligase complexes.90,91


*    Adjusted Changes of UPS to Intracellular Ca2+ Levels
up arrowTop
up arrowAbstract
up arrowIntroduction
up arrowUbiquitin and Ubiquitination
up arrow20S and 26S Proteasomes
up arrowLocalization of Proteasomes...
up arrowPhysiological Roles of...
up arrowRole of the UPS...
up arrowUPS in the Central...
up arrowGeneral Properties and Chemistry...
up arrowChanges in the UPS...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
*Adjusted Changes of UPS...
down arrowUPS and Mitochondrial Function
down arrowAdjusted Changes of UPS...
down arrowRelationship of UPS and...
down arrowUPS and Protein Synthesis
down arrowUPS and the Damage...
down arrowUPS and Protein Kinases
down arrowUPS and Gene-Mediated Effects...
down arrowUPS and Heat Shock...
down arrowUPS and Ischemic Core...
down arrowProteasome Inhibitors in Animal...
down arrowTime-Dependent and Cell...
down arrowLimitations, Future Areas, and...
down arrowReferences
 
The effects of proteasome inhibitors on intracellular Ca2+ levels were tested in murine neocortical cultures and resulted in widespread neuronal death associated with a reduction in intracellular free calcium associated with intracellular calcium starvation.92


*    UPS and Mitochondrial Function
up arrowTop
up arrowAbstract
up arrowIntroduction
up arrowUbiquitin and Ubiquitination
up arrow20S and 26S Proteasomes
up arrowLocalization of Proteasomes...
up arrowPhysiological Roles of...
up arrowRole of the UPS...
up arrowUPS in the Central...
up arrowGeneral Properties and Chemistry...
up arrowChanges in the UPS...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of UPS...
*UPS and Mitochondrial Function
down arrowAdjusted Changes of UPS...
down arrowRelationship of UPS and...
down arrowUPS and Protein Synthesis
down arrowUPS and the Damage...
down arrowUPS and Protein Kinases
down arrowUPS and Gene-Mediated Effects...
down arrowUPS and Heat Shock...
down arrowUPS and Ischemic Core...
down arrowProteasome Inhibitors in Animal...
down arrowTime-Dependent and Cell...
down arrowLimitations, Future Areas, and...
down arrowReferences
 
Proteasome inhibition induces multiple effects on mitochondria: mitochondrial membrane potential ({Delta}{psi}m) reduction, dense mitochondrial deposition, cytochrome-c release into the cytosol with secondary dilated rough ER, formation of cytoplasmic vacuoles, and caspase activation inducing neuronal apoptosis.38,47,93,94


*    Adjusted Changes of UPS to Reactive Oxygen Species
up arrowTop
up arrowAbstract
up arrowIntroduction
up arrowUbiquitin and Ubiquitination
up arrow20S and 26S Proteasomes
up arrowLocalization of Proteasomes...
up arrowPhysiological Roles of...
up arrowRole of the UPS...
up arrowUPS in the Central...
up arrowGeneral Properties and Chemistry...
up arrowChanges in the UPS...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of UPS...
up arrowUPS and Mitochondrial Function
*Adjusted Changes of UPS...
down arrowRelationship of UPS and...
down arrowUPS and Protein Synthesis
down arrowUPS and the Damage...
down arrowUPS and Protein Kinases
down arrowUPS and Gene-Mediated Effects...
down arrowUPS and Heat Shock...
down arrowUPS and Ischemic Core...
down arrowProteasome Inhibitors in Animal...
down arrowTime-Dependent and Cell...
down arrowLimitations, Future Areas, and...
down arrowReferences
 
After cerebral ischemia reperfusion injury, there is a time-dependent decrease in proteasome activity in the affected area that is associated with posttranslational changes and not with decreased expression of proteasome subunits.38,55 Indeed, reactive oxygen species (ROS) are known to modify several proteasome subunits ({alpha}1, {alpha}2, and {alpha}4) and impair proteasome activity.95,96 20S proteasomes degrade mildly oxidized proteins without previous ubiquitination; however, they are unable to degrade extensively oxidized proteins.97 Moreover, oxidative damage enhances the effects of proteasome inhibition, leading to protein aggregation and cell death.98 Because metal ions appear to be delocalized from the proteins in the ischemic and postischemic phase, the effect of oxidative stress induced by neurotoxic metal ions on the properties of the brain 20S proteasome has also been studied, showing that metal-catalyzed oxidation strongly affects the functions of the brain 20S proteasome: TL activity showed gradual activation whereas ChTL and PGPH activities were substantially inhibited.89 At the same time, the intracellular redox status, probably through the level of oxidized proteins, is an important element that can either activate or downregulate the 20S proteasome ChTL activity99 acting by a feedback mechanism, because the antioxidant system is also subjected to the proteasome-dependent proteolysis.100


*    Relationship of UPS and Glutamate Excitotoxicity
up arrowTop
up arrowAbstract
up arrowIntroduction
up arrowUbiquitin and Ubiquitination
up arrow20S and 26S Proteasomes
up arrowLocalization of Proteasomes...
up arrowPhysiological Roles of...
up arrowRole of the UPS...
up arrowUPS in the Central...
up arrowGeneral Properties and Chemistry...
up arrowChanges in the UPS...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of UPS...
up arrowUPS and Mitochondrial Function
up arrowAdjusted Changes of UPS...
*Relationship of UPS and...
down arrowUPS and Protein Synthesis
down arrowUPS and the Damage...
down arrowUPS and Protein Kinases
down arrowUPS and Gene-Mediated Effects...
down arrowUPS and Heat Shock...
down arrowUPS and Ischemic Core...
down arrowProteasome Inhibitors in Animal...
down arrowTime-Dependent and Cell...
down arrowLimitations, Future Areas, and...
down arrowReferences
 
The early phase of necrosis induced as a result of glutamate neurotoxicity apparently does not require proteasome activation.101,102 Proteasome inhibitors do not affect the NF-{kappa}B activation in rat striatal neurons by NMDA receptor stimulation involving I{kappa}B{alpha} degradation by a caspase-dependent mechanism.102 Proteasome inhibition can prevent cytochrome-c release in cerebellar granular cells undergoing apoptosis, thus improving cell survival but not necrosis.101 However, glutamate receptor antagonists might also exacerbate proteasome inhibition-induced neuronal death.92


*    UPS and Protein Synthesis
up arrowTop
up arrowAbstract
up arrowIntroduction
up arrowUbiquitin and Ubiquitination
up arrow20S and 26S Proteasomes
up arrowLocalization of Proteasomes...
up arrowPhysiological Roles of...
up arrowRole of the UPS...
up arrowUPS in the Central...
up arrowGeneral Properties and Chemistry...
up arrowChanges in the UPS...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of UPS...
up arrowUPS and Mitochondrial Function
up arrowAdjusted Changes of UPS...
up arrowRelationship of UPS and...
*UPS and Protein Synthesis
down arrowUPS and the Damage...
down arrowUPS and Protein Kinases
down arrowUPS and Gene-Mediated Effects...
down arrowUPS and Heat Shock...
down arrowUPS and Ischemic Core...
down arrowProteasome Inhibitors in Animal...
down arrowTime-Dependent and Cell...
down arrowLimitations, Future Areas, and...
down arrowReferences
 
There is very little known about rates of protein degradation after ischemia. There is a marked decrease in Ub immunoreactivity in gerbil and rat hippocampus within hours after global ischemia, which then recovers over the next 1 to 3 days in all cell types except CA1 cells that are destined for death.80,103 During focal ischemia, blocking proteasome activity was extremely protective to the core of the lesion.104 One explanation for this is that generalized protein degradation makes a major contribution to ischemic core damage. Indeed, protein content within the core of a focal lesion is severely reduced. However, another possible explanation is that proteasome activity is damaging because it allows NF-{kappa}B activation.105 Cell death might not result from a functional defect in 1 or more key processes; rather, it may result from continued activation of perpetrators set in motion by the ischemia, with ultimate breakdown of the cell as a unit. Besides the UPS, during ischemia there may be an activation of calpains and lysosomal cathepsins, which degrade material delivered by autophagy. Thus, at the doses used in vivo, it is possible that proteasome inhibitors are also blocking activity of other proteolytic systems, either directly or indirectly.


*    UPS and the Damage to the Cytoskeleton
up arrowTop
up arrowAbstract
up arrowIntroduction
up arrowUbiquitin and Ubiquitination
up arrow20S and 26S Proteasomes
up arrowLocalization of Proteasomes...
up arrowPhysiological Roles of...
up arrowRole of the UPS...
up arrowUPS in the Central...
up arrowGeneral Properties and Chemistry...
up arrowChanges in the UPS...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of UPS...
up arrowUPS and Mitochondrial Function
up arrowAdjusted Changes of UPS...
up arrowRelationship of UPS and...
up arrowUPS and Protein Synthesis
*UPS and the Damage...
down arrowUPS and Protein Kinases
down arrowUPS and Gene-Mediated Effects...
down arrowUPS and Heat Shock...
down arrowUPS and Ischemic Core...
down arrowProteasome Inhibitors in Animal...
down arrowTime-Dependent and Cell...
down arrowLimitations, Future Areas, and...
down arrowReferences
 
The microtubule dissolution contributes significantly to apoptotic or necrotic cell death.106 Proteasome inhibitors prevent Wallerian degeneration in vitro and in vivo, stabilizing microtubular cytoskeleton in the axons.107,108 Because increased proteolysis of different cytoskeletal elements is one of the early events in the penumbra of an ischemic lesion, it is likely that such a mechanism also contributes to the neuroprotection in stroke.


*    UPS and Protein Kinases
up arrowTop
up arrowAbstract
up arrowIntroduction
up arrowUbiquitin and Ubiquitination
up arrow20S and 26S Proteasomes
up arrowLocalization of Proteasomes...
up arrowPhysiological Roles of...
up arrowRole of the UPS...
up arrowUPS in the Central...
up arrowGeneral Properties and Chemistry...
up arrowChanges in the UPS...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of UPS...
up arrowUPS and Mitochondrial Function
up arrowAdjusted Changes of UPS...
up arrowRelationship of UPS and...
up arrowUPS and Protein Synthesis
up arrowUPS and the Damage...
*UPS and Protein Kinases
down arrowUPS and Gene-Mediated Effects...
down arrowUPS and Heat Shock...
down arrowUPS and Ischemic Core...
down arrowProteasome Inhibitors in Animal...
down arrowTime-Dependent and Cell...
down arrowLimitations, Future Areas, and...
down arrowReferences
 
Permanent or long-term inactivation of protein kinases or phosphatases could lead to initiation of apoptosis or could lead to the permanent alteration of proteins involved in cell membrane or mitochondrial function, the cytoskeleton, or protein synthesis. Such effects could thus make a major contribution to ischemic cell damage.106 The UPS has been implicated in regulating the levels of many cellular proteins of the signal transduction pathways.109 There is a direct relationship between the phosphorylation/dephosphorylation cascade of the signal transduction pathways and the targeting of the regulatory proteins for ubiquitination. These interacting systems are seen for protein kinase C,110–112 Ca2+-calmodulin–dependent protein kinase,113,114 MAP kinases,115,116 cyclin-dependent kinases,117–119 and calcineurin (calmodulin-dependent phosphatase).120 Proteasome inhibitors demonstrate that many proteins of the signal transduction pathways are regulated by degradation via the UPS, and their use is associated with multiple perturbations in expression/activation of signaling-related and survival-related proteins.


*    UPS and Gene-Mediated Effects Acting on NF-{kappa}B
up arrowTop
up arrowAbstract
up arrowIntroduction
up arrowUbiquitin and Ubiquitination
up arrow20S and 26S Proteasomes
up arrowLocalization of Proteasomes...
up arrowPhysiological Roles of...
up arrowRole of the UPS...
up arrowUPS in the Central...
up arrowGeneral Properties and Chemistry...
up arrowChanges in the UPS...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of UPS...
up arrowUPS and Mitochondrial Function
up arrowAdjusted Changes of UPS...
up arrowRelationship of UPS and...
up arrowUPS and Protein Synthesis
up arrowUPS and the Damage...
up arrowUPS and Protein Kinases
*UPS and Gene-Mediated Effects...
down arrowUPS and Heat Shock...
down arrowUPS and Ischemic Core...
down arrowProteasome Inhibitors in Animal...
down arrowTime-Dependent and Cell...
down arrowLimitations, Future Areas, and...
down arrowReferences
 
Free radical damage after ischemia is probably partially mediated by NF-{kappa}B. Although the NF-{kappa}B pathway can be antiapoptotic in some conditions,121,122 there is evidence that it is damaging after ischemia. NF-{kappa}B is activated in the core and penumbra 1 day after 90-minute temporary focal ischemia of the cortex.123 MLN-519 strongly attenuates damage measured 24 hours after 2-hour ischemia,104 which may reflect prevention of damage via increased global proteolysis, or it may reflect the prevention of NF-{kappa}B activation. However, because the proteasome pathway is required for NF-{kappa}B activation, the result may reflect the importance of NF-{kappa}B in focal damage. If so, it shows that the ischemic core is most susceptible to damage via this system. NF-{kappa}B drives the transcription of many proinflammatory cytokines (IL-1ß and TNF-{alpha}), enzymes (COX-2, iNOS), which are damaging in focal and global ischemia, and also of cell adhesion molecules, such as ICAM-1 and selectins of endothelial cells, fibronectin, and laminin of the extracellular matrix, and integrins and l-selectins of neutrophiles.122,124 Both these responses were blunted by proteasome inhibitors.104,123,125


*    UPS and Heat Shock Protein
up arrowTop
up arrowAbstract
up arrowIntroduction
up arrowUbiquitin and Ubiquitination
up arrow20S and 26S Proteasomes
up arrowLocalization of Proteasomes...
up arrowPhysiological Roles of...
up arrowRole of the UPS...
up arrowUPS in the Central...
up arrowGeneral Properties and Chemistry...
up arrowChanges in the UPS...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of UPS...
up arrowUPS and Mitochondrial Function
up arrowAdjusted Changes of UPS...
up arrowRelationship of UPS and...
up arrowUPS and Protein Synthesis
up arrowUPS and the Damage...
up arrowUPS and Protein Kinases
up arrowUPS and Gene-Mediated Effects...
*UPS and Heat Shock...
down arrowUPS and Ischemic Core...
down arrowProteasome Inhibitors in Animal...
down arrowTime-Dependent and Cell...
down arrowLimitations, Future Areas, and...
down arrowReferences
 
The pattern of heat shock protein (Hsp) expression after ischemia is very similar to that of the immediate early genes. Messenger RNA for Hsp-70 and Hsp-90 begin to rise within a few minutes of the ischemic insult in all regions and persist.106 Hsps may confer resistance to ischemia by preserving proteasome function and attenuating the toxicity of proteasome inhibition reducing oxidative stress.38 It is suggested that Hsp-90 induces conformational changes that affect the ChT-L and PGPH activities,126 depending on the activation state of the proteasome.127 The accumulation of misfolded proteins in the cytosol leads to increased Hsps expression, whereas accumulation of such proteins in the ER (ER stress) triggers the unfolded protein response, stimulating the expression of many ER resident chaperones. Proteasome inhibitors block the rapid degradation of abnormal cytosolic and ER-associated proteins,22 therefore inducing the expression of various Hsps and ER chaperones.


*    UPS and Ischemic Core Damage
up arrowTop
up arrowAbstract
up arrowIntroduction
up arrowUbiquitin and Ubiquitination
up arrow20S and 26S Proteasomes
up arrowLocalization of Proteasomes...
up arrowPhysiological Roles of...
up arrowRole of the UPS...
up arrowUPS in the Central...
up arrowGeneral Properties and Chemistry...
up arrowChanges in the UPS...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of UPS...
up arrowUPS and Mitochondrial Function
up arrowAdjusted Changes of UPS...
up arrowRelationship of UPS and...
up arrowUPS and Protein Synthesis
up arrowUPS and the Damage...
up arrowUPS and Protein Kinases
up arrowUPS and Gene-Mediated Effects...
up arrowUPS and Heat Shock...
*UPS and Ischemic Core...
down arrowProteasome Inhibitors in Animal...
down arrowTime-Dependent and Cell...
down arrowLimitations, Future Areas, and...
down arrowReferences
 
Ischemic core damage does seem to be prevented by proteasome inhibitors. This is a somewhat surprising result, because the inhibitor actually extended penumbral damage somewhat (possibly because of the prevention of normally antiapoptotic effect of NF-{kappa}B). It seems quite unlikely that the effect at the core is caused by NF-{kappa}B blockade. Damage there seems unlikely to require synthesis of new proteins. Thus the result suggests that the activation of 20S proteasome contributes to cell death by causing breakdown of specific proteins.104,125


*    Proteasome Inhibitors in Animal Models of Focal Ischemia and Reperfusion Injury
up arrowTop
up arrowAbstract
up arrowIntroduction
up arrowUbiquitin and Ubiquitination
up arrow20S and 26S Proteasomes
up arrowLocalization of Proteasomes...
up arrowPhysiological Roles of...
up arrowRole of the UPS...
up arrowUPS in the Central...
up arrowGeneral Properties and Chemistry...
up arrowChanges in the UPS...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of UPS...
up arrowUPS and Mitochondrial Function
up arrowAdjusted Changes of UPS...
up arrowRelationship of UPS and...
up arrowUPS and Protein Synthesis
up arrowUPS and the Damage...
up arrowUPS and Protein Kinases
up arrowUPS and Gene-Mediated Effects...
up arrowUPS and Heat Shock...
up arrowUPS and Ischemic Core...
*Proteasome Inhibitors in Animal...
down arrowTime-Dependent and Cell...
down arrowLimitations, Future Areas, and...
down arrowReferences
 
It is clear that there are numerous mechanisms of action for proteasome inhibitors in protecting neurones and glia from ischemic damage. Reduction of cerebral infarct volume by proteasome inhibitors may depend on a combination of effects and from the net balance of their positive and negative effects in modulating the cellular metabolic pathways. Proteasome inhibitors have been tested in different stroke models (Table 2), although not all their possible mechanisms of action are clearly established. Only for MLN-519 is a relatively complete evaluation of neuroprotective properties available.59,61 It has demonstrated consistently reduction of cerebral infarct volume in several rat models of focal brain ischemia,61,104 with evidence of a dose-response effect within readily achieved serum levels and a prolonged time window of up to 6 hours after onset of ischemia, which is highly favorable for a neuroprotective drug.104,125 In rodent models of ischemia, MLN-519 attenuated the expression of the inflammatory cascade acting on NF-{kappa}B pathway, reduced the invasion of leukocytes, and, hence, limited tissue damage.104,125 When MLN-519 was combined with tissue plasminogen activator in a rodent embolic stroke model, it could not only reduce infarct volume and improve neurological outcome 1 week after the ischemic episode but also could eliminate the hemorrhage associated with tissue plasminogen activator treatment given 6 hours after vessel occlusion.128 The apparent neuroprotective effect was also evident in reducing inflammatory response in a model of cerebral hemorrhage.61


View this table:
[in this window]
[in a new window]
 
TABLE 2. Animal Models of Cerebral Ischemia and Proteasome Inhibitor Treatment


*    Time-Dependent and Cell-Dependent Effects of Proteasome Inhibitors
up arrowTop
up arrowAbstract
up arrowIntroduction
up arrowUbiquitin and Ubiquitination
up arrow20S and 26S Proteasomes
up arrowLocalization of Proteasomes...
up arrowPhysiological Roles of...
up arrowRole of the UPS...
up arrowUPS in the Central...
up arrowGeneral Properties and Chemistry...
up arrowChanges in the UPS...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of UPS...
up arrowUPS and Mitochondrial Function
up arrowAdjusted Changes of UPS...
up arrowRelationship of UPS and...
up arrowUPS and Protein Synthesis
up arrowUPS and the Damage...
up arrowUPS and Protein Kinases
up arrowUPS and Gene-Mediated Effects...
up arrowUPS and Heat Shock...
up arrowUPS and Ischemic Core...
up arrowProteasome Inhibitors in Animal...
*Time-Dependent and Cell...
down arrowLimitations, Future Areas, and...
down arrowReferences
 
At the present, it is difficult to outline a single and clearly defined role of the UPS in cerebral ischemia and to establish what are the exact reasons why in stroke models the proteasome inhibitors have an apparently well-defined neuroprotective effect. Some reasons can be postulated.

First, proteasome inhibition occurs during cerebral ischemia reperfusion injury and is mediated, at least in part, by oxidative stress, which also directly activates NF-{kappa}B.38,92,129 Proteasome inhibition may be the means by which oxidative stress mediates neuronal cell death. After cerebral ischemia reperfusion injury, there is a time-dependent decrease in proteasome activity that is not associated with decreased expression of proteasome subunits. At the same time, a time- and dose-dependent proteasome inhibition promotes neuronal survival after stroke and helps neurons to maintain their physiological functions. Probably, proteasome activity plays a double role in ischemic damage. Postischemic impairment of proteasome activity leads to accumulation of Ub conjugates, contributing to loss of neuronal function; however, proteasome activity is associated with a developing inflammatory response by activation of NF-{kappa}B–mediated transcription in neuronal and non-neuronal cells.130 Although neurons can withstand relatively long periods with intracellular accumulations of ubiquitinated proteins such as found in neurodegenerative disorders,131–134 they are very sensitive to damage elicited by an inflammatory response. Therefore, proteasome inhibitors are considered to be of interest in stroke medicine, because they are able to prevent NF-{kappa}B activation135 and therefore reduce the ischemic damage after stroke.136

Second, probably, proteasome inhibition prevents the death of neurons immediately after cerebral ischemia but may start to kill them thereafter. It is demonstrated that prolonged proteasome inhibition has detrimental effects in cultured neuronal cells.47,54,101,137 This phenomenon is observed in different systems. Proteasome inhibition also induces a time-dependent and dose-dependent increase in protein poly-ADP-ribosylation in the neural PC6 cell line and in primary hippocampal neuron cultures54 and dopamine neurotoxicity increases in the presence of proteasome inhibitors in a neural PC12 cell line.138 By contrast, repair mediated by UPS appears to be long-lasting. This is in agreement with the physiological function of proteasome in the nervous system during development.40,42,43

Third, proteasome inhibitors have, probably, a cell specificity of effect because regulated protein degradation mediated by the proteasomes evidently play distinct and well-defined roles on the various pathways: some cells are sensitive to proteasome inhibition and others are not.22 Hypoxic endothelia showed a >10-fold increase in sensitivity to inhibitors of proteasome activation.74

Fourth, a relevant effect of proteasome inhibitors is the inhibition of gene-mediated effects acting on NF-{kappa}B. However, the role of NF-{kappa}B in the brain is unclear. In vitro, NF-{kappa}B activation can be either protective or deleterious. Cell culture studies have clearly shown that activation of NF-{kappa}B in neurons protects them against excitotoxic and metabolic insults relevant to the pathogenesis of stroke.139 Data from studies of mice lacking the p50 subunit of NF-{kappa}B suggest that, overall, NF-{kappa}B activation enhances ischemic neuronal death, but its effects differ between cell types such that, whereas activation of NF-{kappa}B in microglia promotes ischemic neuronal degeneration, activation of NF-{kappa}B in neurons may increase their survival after a stroke.139 The neuroprotective effects of proteasome inhibitors in vivo probably involve non-neuronal mechanisms, primarily in the vasculature within the ischemic area by the downregulated expression of genes in microvascular endothelial cells that encode for inflammatory cytokines and adhesion molecules.59,61,140 Radiolabeled proteasome inhibitors did not show any evidence of brain penetration when administered at times when blood–brain barrier integrity was weakest (at 2 and 24 hours after injury) in an ischemic stroke model.104 At the same time, proteasome inhibitors prevent the disruption of the integrity of the microvascular beds, partially based on their inhibitory action on matrix metalloproteinases.141


*    Limitations, Future Areas, and New Perspectives: Hypothesis of the Dual Role of the UPS in Stroke
up arrowTop
up arrowAbstract
up arrowIntroduction
up arrowUbiquitin and Ubiquitination
up arrow20S and 26S Proteasomes
up arrowLocalization of Proteasomes...
up arrowPhysiological Roles of...
up arrowRole of the UPS...
up arrowUPS in the Central...
up arrowGeneral Properties and Chemistry...
up arrowChanges in the UPS...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of UPS...
up arrowUPS and Mitochondrial Function
up arrowAdjusted Changes of UPS...
up arrowRelationship of UPS and...
up arrowUPS and Protein Synthesis
up arrowUPS and the Damage...
up arrowUPS and Protein Kinases
up arrowUPS and Gene-Mediated Effects...
up arrowUPS and Heat Shock...
up arrowUPS and Ischemic Core...
up arrowProteasome Inhibitors in Animal...
up arrowTime-Dependent and Cell...
*Limitations, Future Areas, and...
down arrowReferences
 
It is clear that the proteasome represents a central target for the processing and metabolism of multiple proteins whose critical roles in cellular function are being elucidated through the use of selective inhibitors. To avoid eliciting the significant side effects associated with complete inhibition of the proteasome (because of its central role in many cellular functions) and the possible immunosuppressive effects (with increased risk of infection and cancer) from persistent suppression of NF-{kappa}B activation, it is, however, critical that we understand how to partially and temporally attenuate proteasome function to elicit the desired therapeutic effect before any large-scale use in humans. Perhaps attention to specific aspects may provide more promise for neuroprotective efficacy than the simpler and less specific proteasome inhibition. The interaction of these events is complex, and the outcome of therapeutic interventions aimed at these elements of cellular injury is uncertain without more rational and specific targeting of these mechanisms and knowledge of the underlying state of the organism with respect to these factors.

Cytoprotective therapies, based on blockade of proteasome, are suitable for use in human emergency medicine. However, an excessive inhibition could counterbalance the apparent positive effect of experimental data and produce a negative result in clinical practice with a strict therapeutic window for the protective effect of proteasome inhibition in humans.

In conclusion, proteasome inhibitors are promising neuroprotective agents. The preclinical profile is superior to many previously investigated compounds and is robust in the hands of different investigators. However, more data on their pharmacokinetics, safety profile, and toxicity are necessary before entering in a more rigorous test of clinical efficacy.


*    Acknowledgments
 
C.W. is a recipient of the American Heart Association–Texas Affiliate beginning grant-in-aid 0365148Y.

Received October 14, 2003; revision received December 31, 2003; accepted February 9, 2004.


*    References
up arrowTop
up arrowAbstract
up arrowIntroduction
up arrowUbiquitin and Ubiquitination
up arrow20S and 26S Proteasomes
up arrowLocalization of Proteasomes...
up arrowPhysiological Roles of...
up arrowRole of the UPS...
up arrowUPS in the Central...
up arrowGeneral Properties and Chemistry...
up arrowChanges in the UPS...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of Ubiquitin...
up arrowAdjusted Changes of UPS...
up arrowUPS and Mitochondrial Function
up arrowAdjusted Changes of UPS...
up arrowRelationship of UPS and...
up arrowUPS and Protein Synthesis
up arrowUPS and the Damage...
up arrowUPS and Protein Kinases
up arrowUPS and Gene-Mediated Effects...
up arrowUPS and Heat Shock...
up arrowUPS and Ischemic Core...
up arrowProteasome Inhibitors in Animal...
up arrowTime-Dependent and Cell...
up arrowLimitations, Future Areas, and...
*References
 
1. del Zoppo G, Ginis I, Hallenbeck JM, Iadecola C, Wang X, Feuerstein GZ. Inflammation and stroke: putative role for cytokines, adhesion molecules and iNOS in brain response to ischemia. Brain Pathol. 2000; 10: 95–112.[Medline] [Order article via Infotrieve]

2. Stanimirovic D, Satoh K. Inflammatory mediators of cerebral endothelium: a role in ischemic brain inflammation. Brain Pathol. 2000; 10: 113–126.[Medline] [Order article via Infotrieve]

3. Jean W, Spellman S, Nussbaum E, Low W. Reperfusion injury after focal cerebral ischemia: the role of inflammation and the therapeutic horizon. Neurosurgery. 2000; 43: 1382–1396.

4. Palombella VJ, Rando OJ, Goldberg AL, Maniatis T. The ubiquitin-proteasome pathway is required for processing the NF-kB1 precursor protein and the activation of NF-kB. Cell. 1994; 78: 773–785.[CrossRef][Medline] [Order article via Infotrieve]

5. Glickman MH, Ciechanover A. The ubiquitin-proteasome proteolytic pathway: destruction for the sake of construction. Physiol Rev. 2002; 82: 373–428.[Abstract/Free Full Text]

6. DeMartino GN, Slaughter CA. The proteasome, a novel protease regulated by multiple mechanisms. J Biol Chem. 1999; 274: 22123–22126.[Free Full Text]

7. Coux O, Tanaka K, Goldberg AL. Structure and functions of the 20S and 26S proteasomes. Annu Rev Biochem. 1996; 65: 801–847.[CrossRef][Medline] [Order article via Infotrieve]

8. Orlowski M, Wilk S. Catalytic activities of the 20 S proteasome, a multicatalytic proteinase complex. Arch Biochem Biophys. 2000; 383: 1–16.[CrossRef][Medline] [Order article via Infotrieve]

9. Liu CW, Millen L, Roman TB, Xiong H, Gilbert HF, Noiva R, DeMartino GN, Thomas PJ. Conformational remodeling of proteasomal substrates by PA700, the 19 S regulatory complex of the 26 S proteasome. J Biol Chem. 2002; 277: 26815–26820.[Abstract/Free Full Text]

10. Yao T, Cohen RE. A cryptic protease couples deubiquitination and degradation by the proteasome. Nature. 2002; 419: 403–407.[CrossRef][Medline] [Order article via Infotrieve]

11. Saeki Y, Sone T, Tohe A, Yokosawa H. Identification of ubiquitin-like protein-binding subunits of the 26S proteasome. Biochem Biophys Res Commun. 2002; 296: 813–819.[CrossRef][Medline] [Order article via Infotrieve]

12. Glickman MH, Rubin DM, Coux O, Wefes I, Pfeifer G, Cjeka Z, Baumeister W, Fried VA, Finley D. A subcomplex of the proteasome regulatory particle required for ubiquitin-conjugate degradation and related to the COP9-signalosome and eIF3. Cell. 1998; 94: 615–623.[CrossRef][Medline] [Order article via Infotrieve]

13. Liu CW, Corboy MJ, DeMartino GN, Thomas PJ. Endoproteolytic activity of the proteasome. Science. 2003; 299: 408–411.[Abstract/Free Full Text]

14. Shringarpure R, Grune T, Mehlhase J, Davies KJ. Ubiquitin conjugation is not required for the degradation of oxidized proteins by proteasome. J Biol Chem. 2003; 278: 311–318.[Abstract/Free Full Text]

15. Hendil KB. The 19 S multicatalytic "prosome" proteinase is a constitutive enzyme in HeLa cells. Biochem Int. 1988; 17: 471–477.[Medline] [Order article via Infotrieve]

16. Wojcik C, DeMartino GN. Intracellular localization of proteasomes. Int J Biochem Cell Biol. 2003; 35: 579–589.[CrossRef][Medline] [Order article via Infotrieve]

17. Wojcik C, Schroeter D, Wilk S, Lamprecht J, Paweletz N. Ubiquitin-mediated proteolysis centers in HeLa cells: indication from studies of an inhibitor of the chymotrypsin-like activity of the proteasome. Eur J Cell Biol. 1996; 71: 311–318.[Medline] [Order article via Infotrieve]

18. Wojcik C. On the spatial organization of ubiquitin-dependent proteolysis in HeLa cells. Folia Histochem Cytobiol. 1997; 35: 117–118.[Medline] [Order article via Infotrieve]

19. Johnston JA, Ward CL, Kopito RR. Aggresomes: a cellular response to misfolded proteins. J Cell Biol. 1998; 143: 1883–1898.[Abstract/Free Full Text]

20. Fujiwara T, Tanaka K, Orino E, Yoshimura T, Kumatori A, Tamura T, Chung CH, Nakai T, Yamaguchi K, Shin S. Proteasomes are essential for yeast proliferation. cDNA cloning and gene disruption of two major subunits. J Biol Chem. 1990; 265: 16604–16613.[Abstract/Free Full Text]

21. Schnall R, Mannhaupt G, Stucka R, Tauer R, Ehnle S, Schwarzlose C, Vetter I, Feldmann H. Identification of a set of yeast genes coding for a novel family of putative ATPases with high similarity to constituents of the 26S protease complex. Yeast. 1994; 10: 1141–1155.[CrossRef][Medline] [Order article via Infotrieve]

22. Wojcik C. Regulation of apoptosis by the ubiquitin and proteasome pathway. J Cell Mol Med. 2002; 6: 25–48.[Medline] [Order article via Infotrieve]

23. Schubert U, Anton LC, Gibbs J, Norbury CC, Yewdell JW, Bennink JR. Rapid degradation of a large fraction of newly synthesized proteins by proteasomes. Nature. 2000; 404: 770–774.[CrossRef][Medline] [Order article via Infotrieve]

24. Kopito RR. Aggresomes, inclusion bodies and protein aggregation. Trends Cell Biol. 2000; 10: 524–530.[CrossRef][Medline] [Order article via Infotrieve]

25. Rock KL, Gramm C, Rothstein L, Clark K, Stein R, Dick L, Hwang D, Goldberg AL. Inhibitors of the proteasome block the degradation of most cell proteins and the generation of peptides presented on MHC class I molecules. Cell. 1994; 78: 761–771.[CrossRef][Medline] [Order article via Infotrieve]

26. Rivett AJ, Bose S, Brooks P, Broadfoot KI. Regulation of proteasome complexes by gamma-interferon and phosphorylation. Biochimie. 2001; 83: 363–366.[Medline] [Order article via Infotrieve]

27. Nathan C. Points of control in inflammation. Nature. 2002; 420: 846–852.[CrossRef][Medline] [Order article via Infotrieve]

28. Priller J, Dirnagl U. Inflammation in stroke–a potential target for neuroprotection? Ernst Schering Res Found Workshop. 2002: 133–157.

29. Di Napoli M, Papa F, Bocola V. Prognostic influence of increased C-reactive protein and fibrinogen levels in ischemic stroke. Stroke. 2001; 32: 133–138.[Abstract/Free Full Text]

30. Di Napoli M, Papa F, Bocola V. C-reactive protein in ischemic stroke. An independent prognostic factor. Stroke. 2001; 32: 981–985.

31. Di Napoli M, Papa F. Inflammation, hemostatic markers, and antithrombotic agents in relation to long-term risk of new cardiovascular events in first-ever ischemic stroke patients. Stroke. 2002; 33: 1763–1771.[Abstract/Free Full Text]

32. Perkins ND. The Rel/NF-{kappa}B family: friend and foe. Trends Biochem Sci. 2000; 25: 434–440.[CrossRef][Medline] [Order article via Infotrieve]

33. Chen Z, Hagler J, Palombella VJ, Melandri F, Scherer D, Ballard D, Maniatis T. Signal-induced site-specific phosphorylation targets I{kappa}B{alpha} to the ubiquitin-proteasome pathway. Genes Dev. 1995; 9: 1586–1597.[Abstract/Free Full Text]

34. Lin L, DeMartino GN, Greene WC. Cotranslational biogenesis of NF-{kappa}B p50 by the 26S proteasome. Cell. 1998; 92: 819–828.[CrossRef][Medline] [Order article via Infotrieve]

35. Rape M, Jentsch S. Taking a bite: proteasomal protein processing. Nat Cell Biol. 2002; 4: E113–E116.[CrossRef][Medline] [Order article via Infotrieve]

36. Amir RE, Iwai K, Ciechanover A. The NEDD8 pathway is essential for SCF (beta-TrCP)-mediated ubiquitination and processing of the NF-{kappa}B precursor p105. J Biol Chem. 2002; 277: 23253–23259.[Abstract/Free Full Text]

37. Wang C, Deng L, Hong M, Akkaraju GR, Inoue J, Chen ZJ. TAK1 is a ubiquitin-dependent kinase of MKK and IKK. Nature. 2001; 412: 346–351.[CrossRef][Medline] [Order article via Infotrieve]

38. Ding Q, Keller JN. Proteasome inhibition in oxidative stress neurotoxicity: implications for heat shock proteins. J Neurochem. 2001; 77: 1010–1017.[CrossRef][Medline] [Order article via Infotrieve]

39. Klimaschewski L. Ubiquitin-dependent proteolysis in neurons. News Physiol Sci. 2003; 18: 29–33.[Abstract/Free Full Text]

40. Takada K, Kanda T, Ohkawa K, Matsuda M. Ubiquitin and ubiquitin-protein conjugates in PC12h cells: changes during neuronal differentiation. Neurochem Res. 1994; 19: 391–398.[CrossRef][Medline] [Order article via Infotrieve]

41. Obin M, Mesco E, Gong X, Haas AL, Joseph J, Taylor A. Neurite outgrowth in PC12 cells. Distinguishing the roles of ubiquitylation and ubiquitin-dependent proteolysis. J Biol Chem. 1999; 274: 11789–11795.[Abstract/Free Full Text]

42. Wojcik C, Wilk S. Changes in proteasome expression and activity during differentiation of neuronal precursor NTera 2 clone D1 cells. Neurochem Int. 1999; 34: 131–136.[CrossRef][Medline] [Order article via Infotrieve]

43. Flann S, Hawkes RB, Riederer BM, Rider CC, Beesley PW. Changes in ubiquitin immunoreactivity in developing rat brain: a putative role for ubiquitin and ubiquitin conjugates in dendrite outgrowth and differentiation. Neuroscience. 1997; 81: 173–187.[CrossRef][Medline] [Order article via Infotrieve]

44. Watts RJ, Hoopfer ED, Luo L. Axon pruning during drosophila metamorphosis. Evidence for local degeneration and requirement of the ubiquitin-proteasome system. Neuron. 2003; 38: 871–885.[CrossRef][Medline] [Order article via Infotrieve]

45. Meyer EM, West CM, Stevens BR, Chau V, Nguyen MT, Judkins JH. Ubiquitin-directed antibodies inhibit neuronal transporters in rat brain synaptosomes. J Neurochem. 1987; 49: 1815–1819.[CrossRef][Medline] [Order article via Infotrieve]

46. Maufroid JP, Bradshaw RA, Boilly B, Hondermarck H. Nerve growth factor induced neurite outgrowth from amphibian neuroepithelial precursor cells is prevented by dipeptides inhibiting ubiquitin-mediated proteolysis. Int J Dev Biol. 1996; 40: 609–611.[Medline] [Order article via Infotrieve]

47. Qiu JH, Asai A, Chi S, Saito N, Hamada H, Kirino T. Proteasome inhibitors induce cytochrome c-caspase-3-like protease-mediated apoptosis in cultured cortical neurons. J Neurosci. 2000; 20: 259–265.[Abstract/Free Full Text]

48. Ehlers MD. Activity level controls postsynaptic composition and signaling via the ubiquitin-proteasome system. Nat Neurosci. 2003; 6: 231–242.[CrossRef][Medline] [Order article via Infotrieve]

49. Zhao Y, Hegde AN, Martin KC. The ubiquitin proteasome system functions as an inhibitory constraint on synaptic strengthening. Curr Biol. 2003; 13: 887–898.[CrossRef][Medline] [Order article via Infotrieve]

50. Akaishi T, Sawada H, Yokosawa H. Properties of 26S proteasome purified from rat skeletal muscles: comparison with those of 26S proteasome from the rat brain. Biochem Mol Biol Int. 1996; 39: 1017–1021.[Medline] [Order article via Infotrieve]

51. Kamakura K, Ishiura S, Nonaka I, Sugita H. Localization of ingensin in rat central nervous system and skeletal muscle. J Neurosci Res. 1988; 20: 473–478.[CrossRef][Medline] [Order article via Infotrieve]

52. Mengual E, Arizti P, Rodrigo J, Gimenez-Amaya JM, Castano JG. Immunohistochemical distribution and electron microscopic subcellular localization of the proteasome in the rat CNS. J Neurosci. 1996; 16: 6331–6341.[Abstract/Free Full Text]

53. Wilczynski G, Rowinski J, Kolzowska H, Wojcik C. Proteasomes in the neurones of the brain cortex. Folia Histochem Cytobiol. 1996; 34: 86.

54. Keller JN, Markesbery WR. Proteasome inhibition results in increased poly-ADP-ribosylation: implications for neuron death. J Neurosci Res. 2000; 61: 436–442.[CrossRef][Medline] [Order article via Infotrieve]

55. Keller JN, Gee J, Ding Q. The proteasome in brain aging. Ageing Res Rev. 2002; 1: 279–293.[CrossRef][Medline] [Order article via Infotrieve]

56. Liu Y, Fallon L, Lashuel HA, Liu Z, Lansbury PT Jr. The UCH-L1 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson’s disease susceptibility. Cell. 2002; 111: 209–218.[CrossRef][Medline] [Order article via Infotrieve]

57. Nawaz Z, Lonard DM, Smith CL, Lev-Lehman E, Tsai SY, Tsai MJ, O’Malley BW. The Angelman syndrome-associated protein, E6-AP, is a coactivator for the nuclear hormone receptor superfamily. Mol Cell Biol. 1999; 19: 1182–1189.[Abstract/Free Full Text]

58. Trockenbacher A, Suckow V, Foerster J, Winter J, Krauss S, Ropers HH, Schneider R, Schweiger S. MID1, mutated in Opitz syndrome, encodes an ubiquitin ligase that targets phosphatase 2A for degradation. Nat Genet. 2001; 29: 287–294.[CrossRef][Medline] [Order article via Infotrieve]

59. Di Napoli M, Papa F. The proteasome system and proteasome inhibitors in stroke: controlling the inflammatory response. Curr Opin Invest Drug. 2003; 4: 1333–1342.

60. Soucy F, Grenier L, Behnke M, Destree AT, McCormack TA, Adams JK, Plamondon L. A novel and efficient synthesis of a highly active analogue of clastro-lactacystin ß-lactone. J Am Chem Soc. 1999; 121: 9967–9976.[CrossRef]

61. Di Napoli M, Papa F. MLN-519. Millennium/PAION. Curr Opin Invest Drugs. 2003; 4: 333–341.[Medline] [Order article via Infotrieve]

62. Sin N, Meng L, Auth H, Crews CH. Eponemycin analogues: syntheses and use as probes of angiogenesis. Bioorg Med Chem. 1998; 6: 1217-

63. Meng L, Mohan R, Know BHL, Elofsson M, Sin N, Crews CH. Epoxomicin, a potent antitumor and anti-inflammatory natural product, targets the proteasome. Proc Natl Acad Sci U S A. 1999; 96: 10403–10408.[Abstract/Free Full Text]

64. Figueiredo-Pereira ME, Chen WE, Li J, Johdo O. The antitumor drug aclacinomycin A, which inhibits the degradation of ubiquitinated proteins, shows selectivity for the chymotrypsin-like activity of the bovine pituitary 20S proteasome. J Biol Chem. 1996; 271: 16455–16459.[Abstract/Free Full Text]

65. Gao Y, Lecker S, Post MJ, Hietaranta AJ, Li J, Volk R, Li M, Sato K, Saluja AK, Steer ML, Goldberg AL, Simons M. Inhibition of ubiquitin-proteasome pathway-mediated I{kappa}B{alpha} degradation by a naturally occurring antibacterial peptide. J Clin Invest. 2000; 106: 439–448.[Medline] [Order article via Infotrieve]

66. An B, Goldfarb RH, Siman R, Dou QP. Novel dipeptidyl proteasome inhibitors overcome Bcl-2 protective function and selectively accumulate the cyclin-dependent kinase inhibitor p27 and induce apoptosis in transformed, but not normal, human fibroblasts. Cell Death Differ. 1998; 5: 1062–1075.[CrossRef][Medline] [Order article via Infotrieve]

67. Lum RT, Nelson MG, Joly A, Horsma AG, Lee G, Meyer SM, Wick MM, Schow SR. Selective inhibition of the chymotrypsin-like activity of the 20S proteasome by 5-methoxy-1-indanone dipeptide benzamides. Bioorg Med Chem Lett. 1998; 8: 209–214.[CrossRef][Medline] [Order article via Infotrieve]

68. Adams J, Behnke M, Chen S, Cruickshank AA, Dick LR, Grenier L, Klunder JM, Ma YT, Plamondon L, Stein RL. Potent and selective inhibitors of the proteasome: dipeptidyl boronic acids. Bioorg Med Chem Lett. 1998; 8: 333–338.[CrossRef][Medline] [Order article via Infotrieve]

69. Furet P, Imbach P, Fuerest P, Lang M, Noorani M, Zimmermann J, Garcia-Echeverria C. Structure-based optimisation of 2-aminobenzylstatine derivatives: potent and selective inhibitors of the chymotrypsin-like activity of the human 20s proteasome. Bioorg Med Chem Lett. 2002; 12: 1331–1334.[CrossRef][Medline] [Order article via Infotrieve]

70. Bogyo M, McMaster JS, Gaczynska M, Tortorella D, Goldberg A, Ploegh H. Covalent modification of the active site threonine of proteasomal beta subunits and the Escherichia coli homologue Hs1V by a new class of inhibitors. Proc Natl Acad Sci U S A. 1997; 94: 6629–6634.[Abstract/Free Full Text]

71. Schmidtke G, Holzhütter H-G, Bogyo M, Kairiesi N, Grolli M, de Giuli R, Emch S, Groettrup M. How an inhibitor of the HIV-I protease modulates proteasome activity. J Biol Chem. 1999; 274: 35734–35740.[Abstract/Free Full Text]

72. Vannucci SJ, Mummery R, Hawkes RB, Rider CC, Beesley PW. Hypoxia-ischemia induces a rapid elevation of ubiquitin conjugate levels and ubiquitin immunoreactivity in the immature rat brain. J Cereb Blood Flow Metab. 1998; 18: 376–385.[CrossRef][Medline] [Order article via Infotrieve]

73. Schmidt-Kastner R, Zhang BT, Webster K, Kietzmann T, Zhao W, Busto R, Ginsberg MD. Hypoxia-inducible factor-1 (hif-1) in experimental brain ischemia. Sci World J. 2002; 2: 123–124.

74. Zund G, Uezono S, Stahl GL, Dzus AL, McGowan FX, Hickey PR, Colgan SP. Hypoxia enhances induction of endothelial ICAM-1: role for metabolic acidosis and proteasomes. Am J Physiol. 1997; 273: C1571–C1580.[Medline] [Order article via Infotrieve]

75. Stanimirovic D, Zhang W, Howlett C, Lemieux P, Smith C. Inflammatory gene transcription in human astrocytes exposed to hypoxia: roles of the nuclear factor-{kappa}B and autocrine stimulation. J Neuroimmunol. 2001; 119: 365–376.[CrossRef][Medline] [Order article via Infotrieve]

76. Gozal D, Row BW, Kheirandish L, Liu R, Guo SZ, Qiang F, Brittian KR. Increased susceptibility to intermittent hypoxia in aging rats: changes in proteasomal activity, neuronal apoptosis and spatial function. J Neurochem. 2003; 86: 1545–1552.[CrossRef][Medline] [Order article via Infotrieve]

77. Salceda S, Caro J. Hypoxia-inducible factor 1alpha (HIF-1alpha) protein is rapidly degraded by the ubiquitin-proteasome system under normoxic conditions. Its stabilization by hypoxia depends on redox-induced changes. J Biol Chem. 1997; 272: 22642–22647.[Abstract/Free Full Text]

78. Kallio PJ, Wilson WJ, O’Brien S, Makino Y, Poellinger L. Regulation of the hypoxia-inducible transcription factor 1alpha by the ubiquitin-proteasome pathway. J Biol Chem. 1999; 274: 6519–6525.[Abstract/Free Full Text]

79. Li J, Post M, Volk R, Gao Y, Li M, Metais C, Sato K, Tsai J, Aird W, Rosenberg RD, Hampton TG, Sellke F, Carmeliet P, Simons M. PR39, a peptide regulator of angiogenesis. Nat Med. 2000; 6: 49–55.[CrossRef][Medline] [Order article via Infotrieve]

80. Gubellini P, Bisso GM, Ciofi-Luzzatto A, Fortuna S, Lorenzini P, Michalek H, Scarsella G. Ubiquitin-mediated stress response in a rat model of brain transient ischemia/hypoxia. Neurochem Res. 1997; 22: 93–100.[CrossRef][Medline] [Order article via Infotrieve]

81. Ide T, Takada K, Qiu JH, Saito N, Kawahara N, Asai A, Kirino T. Ubiquitin stress response in postischemic hippocampus under nontolerant and tolerant conditions. J Cereb Blood Flow Metab. 1999; 19: 750–756.[CrossRef][Medline] [Order article via Infotrieve]

82. Taglialatela G, Kaufmann JA, Trevino A, Perez-Polo JR. Central nervous system DNA fragmentation induced by the inhibition of nuclear factor {kappa}B. Neuroreport. 1998; 9: 489–493.[Medline] [Order article via Infotrieve]

83. Noga M, Hayashi T, Tanaka J. Gene expressions of ubiquitin and hsp70 following focal ischaemia in rat brain. Neuroreport. 1997; 8: 1239–1241.[Medline] [Order article via Infotrieve]

84. Lee C, Schwartz MP, Prakash S, Iwakura M, Matouschek A. ATP-dependent proteases degrade their substrates by processively unraveling them from the degradation signal. Mol Cell. 2001; 7: 627–637.[CrossRef][Medline] [Order article via Infotrieve]

85. Lam YA, Lawson TG, Velayutham M, Zweier JL, Pickart CM. A proteasomal ATPase subunit recognizes the polyubiquitin degradation signal. Nature. 2002; 416: 763–767.[CrossRef][Medline] [Order article via Infotrieve]

86. Asai A, Tanahashi N, Qiu JH, Saito N, Chi S, Kawahara N, Tanaka K, Kirino T. Selective proteasomal dysfunction in the hippocampal CA1 region after transient forebrain ischemia. J Cereb Blood Flow Metab. 2002; 22: 705–710.[Medline] [Order article via Infotrieve]

87. Mengesdorf T, Jensen PH, Mies G, Aufenberg C, Paschen W. Down-regulation of parkin protein in transient focal cerebral ischemia: a link between stroke and degenerative disease? Proc Natl Acad Sci U S A. 2002; 99: 15042–15047.[Abstract/Free Full Text]

88. Kamikubo T, Hayashi T. Changes in proteasome activity following transient ischemia. Neurochem Int. 1996; 28: 209–212.[CrossRef][Medline] [Order article via Infotrieve]

89. Amici M, Forti K, Nobili C, Lupidi G, Angeletti M, Fioretti E, Eleuteri AM. Effect of neurotoxic metal ions on the proteolytic activities of the 20S proteasome from bovine brain. J Biol Inorg Chem. 2002; 7: 750–756.[CrossRef][Medline] [Order article via Infotrieve]

90. Fruh K, Gossen M, Wang K, Bujard H, Peterson PA, Yang Y. Displacement of housekeeping proteasome subunits by MHC-encoded LMPs: a newly discovered mechanism for modulating the multicatalytic proteinase complex. EMBO J. 1994; 13: 3236–3244.[Medline] [Order article via Infotrieve]

91. Hershko A. Roles of ubiquitin-mediated proteolysis in cell cycle control. Curr Opin Cell Biol. 1997; 9: 788–799.[CrossRef][Medline] [Order article via Infotrieve]

92. Snider BJ, Tee LY, Canzoniero LM, Babcock DJ, Choi DW. NMDA antagonists exacerbate neuronal death caused by proteasome inhibition in cultured cortical and striatal neurons. Eur J Neurosci. 2002; 15: 419–428.[CrossRef][Medline] [Order article via Infotrieve]

93. Wagenknecht B, Hermisson M, Groscurth P, Liston P, Krammer PH, Weller M. Proteasome inhibitor-induced apoptosis of glioma cells involves the processing of multiple caspases and cytochrome c release. J Neurochem. 2000; 75: 2288–2297.[CrossRef][Medline] [Order article via Infotrieve]

94. Ling YH, Liebes L, Zou Y, Perez-Soler R. Reactive oxygen species generation and mitochondrial dysfunction in the apoptotic response to Bortezomib, a novel proteasome inhibitor, in human H460 non-small cell lung cancer cells. J Biol Chem. 2003; 278: 33714–33723.[Abstract/Free Full Text]

95. Bulteau AL, Lundberg KC, Humphries KM, Sadek HA, Szweda PA, Friguet B, Szweda LI. Oxidative modification and inactivation of the proteasome during coronary occlusion/reperfusion. J Biol Chem. 2001; 276: 30057–30063.[Abstract/Free Full Text]

96. Carrard G, Bulteau AL, Petropoulos I, Friguet B. Impairment of proteasome structure and function in aging. Int J Biochem Cell Biol. 2002; 34: 1461–1474.[CrossRef][Medline] [Order article via Infotrieve]

97. Grune T, Merker K, Sandig G, Davies KJ. Selective degradation of oxidatively modified protein substrates by the proteasome. Biochem Biophys Res Commun. 2003; 305: 709–718.[CrossRef][Medline] [Order article via Infotrieve]

98. Demasi M, Davies KJ. Proteasome inhibitors induce intracellular protein aggregation and cell death by an oxygen-dependent mechanism. FEBS Lett. 2003; 542: 89–94.[CrossRef][Medline] [Order article via Infotrieve]

99. Kretz-Remy C, Arrigo AP. Modulation of the chymotrypsin-like activity of the 20S proteasome by intracellular redox status: effects of glutathione peroxidase-1 overexpression and antioxidant drugs. Biol Chem. 2003; 384: 589–595.[CrossRef][Medline] [Order article via Infotrieve]

100. Atlante A, Bobba A, Calissano P, Passarella S, Marra E. The apoptosis/necrosis transition in cerebellar granule cells depends on the mutual relationship of the antioxidant and the proteolytic systems which regulate ROS production and cytochrome c release en route to death. J Neurochem. 2003; 84: 960–971.[CrossRef][Medline] [Order article via Infotrieve]

101. Bobba A, Canu N, Atlante A, Petragallo V, Calissano P, Marra E. Proteasome inhibitors prevent cytochrome c release during apoptosis but not in excitotoxic death of cerebellar granule neurons. FEBS Lett. 2002; 515: 8–12.[CrossRef][Medline] [Order article via Infotrieve]

102. Qin Z, Wang Y, Chasea TN. A caspase-3-like protease is involved in NF-{kappa}B activation induced by stimulation of N-methyl-D-aspartate receptors in rat striatum. Brain Res Mol Brain Res. 2000; 80: 111–122.[Medline] [Order article via Infotrieve]

103. Franklin JL, Johnson EMJ. Control of neuronal size homeostasis by trophic factor-mediated coupling of protein degradation to protein synthesis. J Cell Biol. 1998; 142: 1313–1324.[Abstract/Free Full Text]

104. Phillips JB, Williams AJ, Adams J, Elliott PJ, Tortella FC. Proteasome inhibitor PS519 reduces infarction and attenuates leukocyte infiltration in a rat model of focal cerebral ischemia. Stroke. 2000; 31: 1686–1693.[Abstract/Free Full Text]

105. Baldwin AS Jr. The NF-kB and IkB proteins: new discoveries and insights. Annu Rev Immunol. 1996; 14: 649–681.[CrossRef][Medline] [Order article via Infotrieve]

106. Lipton P. Ischemic cell death in brain neurons. Physiol Review. 1999; 79: 1431–1568.[Abstract/Free Full Text]

107. Conforti L, Tarlton A, Mack TG, Mi W, Buckmaster EA, Wagner D, Perry VH, Coleman MP. A Ufd2/D4Cole1e chimeric protein and overexpression of Rbp7 in the slow Wallerian degeneration (WldS) mouse. Proc Natl Acad Sci U S A. 2000; 97: 11377–11382.[Abstract/Free Full Text]

108. Zhai Q, Wang J, Kim A, Liu Q, Watts R, Hoopfer E, Mitchison T, Luo L, He Z. Involvement of the ubiquitin-proteasome system in the early stages of wallerian degeneration. Neuron. 2003; 39: 217–225.[CrossRef][Medline] [Order article via Infotrieve]

109. Fuchs SY, Fried VA, Ronai Z. Stress-activated kinases regulate protein stability. Oncogene. 1998; 17: 1483–1490.[CrossRef][Medline] [Order article via Infotrieve]

110. Lu Z, Liu D, Hornia A, Devonish W, Pagano M, Foster DA. Activation of protein kinase C triggers its ubiquitination and degradation. Mol Cell Biol. 1998; 18: 839–845.[Abstract/Free Full Text]

111. Okuda H, Saitoh K, Hirai S, Iwai K, Takaki Y, Baba M, Minato N, Ohno S, Shuin T. The von Hippel-Lindau tumor suppressor protein mediates ubiquitination of activated atypical protein kinase C. J Biol Chem. 2001; 276: 43611–43617.[Abstract/Free Full Text]

112. Youdim MB, Amit T, Falach-Yogev M, Am OB, Maruyama W, Naoi M. The essentiality of Bcl-2, PKC and proteasome-ubiquitin complex activations in the neuroprotective-antiapoptotic action of the anti-Parkinson drug, rasagiline. Biochem Pharmacol. 2003; 66: 1635–1641.[CrossRef][Medline] [Order article via Infotrieve]

113. Schulte TW, An WG, Neckers LM. Geldanamycin-induced destabilization of Raf-1 involves the proteasome. Biochem Biophys Res Commun. 1997; 239: 655–659.[CrossRef][Medline] [Order article via Infotrieve]

114. Dimmeler S, Breitschopf K, Haendeler J, Zeiher AM. Dephosphorylation targets Bcl-2 for ubiquitin-dependent degradation: a link between the apoptosome and the proteasome pathway. J Exp Med. 1999; 189: 1815–1822.[Abstract/Free Full Text]

115. Song S, Kim SY, Hong YM, Jo DG, Lee JY, Shim SM, Chung CW, Seo SJ, Yoo YJ, Koh JY, Lee MC, Yates AJ, Ichijo H, Jung YK. Essential role of E2–25K/Hip-2 in mediating amyloid-ß neurotoxicity. Mol Cell. 2003; 12: 553–563.[CrossRef][Medline] [Order article via Infotrieve]

116. Wu HM, Wen HC, Lin WW. Proteasome inhibitors stimulate interleukin-8 expression via Ras and apoptosis signal-regulating kinase-dependent extracellular signal-related kinase and c-Jun N-terminal kinase activation. Am J Respir Cell Mol Biol. 2002; 27: 234–243.[Abstract/Free Full Text]

117. Dai Y, Rahmani M, Grant S. Proteasome inhibitors potentiate leukemic cell apoptosis induced by the cyclin-dependent kinase inhibitor flavopiridol through a SAPK/JNK- and NF-{kappa}B-dependent process. Oncogene. 2003; 22: 7108–7122.[CrossRef][Medline] [Order article via Infotrieve]

118. Patrick GN, Zhou P, Kwon YT, Howley PM, Tsai LH. p35, the neuronal-specific activator of cyclin-dependent kinase 5 (Cdk5) is degraded by the ubiquitin-proteasome pathway. J Biol Chem. 1998; 273: 24057–24064.[Abstract/Free Full Text]

119. Rideout HC, Wang Q, Park DS, Stefanis L. Cyclin-dependent kinase activity is required for apoptotic death but not inclusion formation in cortical neurons after proteasomal inhibition. J Neurosci. 2003; 23: 1237–1245.[Abstract/Free Full Text]

120. Casciati A, Ferri A, Cozzolino M, Celsi F, Nencini M, Rotilio G, Carri MT. Oxidative modulation of nuclear factor-{kappa}B in human cells expressing mutant fALS-typical superoxide dismutases. J Neurochem. 2002; 83: 1019–1029.[CrossRef][Medline] [Order article via Infotrieve]

121. Schulze-Osthoff K, Ferrari D, Riehemann K, Wesselborg S. Regulation of NF-kB activation by MAP kinase cascades. Immunobiology. 1997; 198: 35–49.[Medline] [Order article via Infotrieve]

122. Wallach D. Cell death induction by TNF: a matter of self control. Trends Biochem Sci. 1997; 22: 107–109.[CrossRef][Medline] [Order article via Infotrieve]

123. Salminen A, Liu PK, Hsu Y. Alteration of transcription factor binding activities in the ischemic brain. Biochem Biophys Res Commun. 1994; 212: 939–944.

124. Rothlein R, Czajkowski M, O’Neill MM, Marlin SD, Mainlofi E, Merluzzi VJ. Induction of intracellular adhesion molecule 1 on primary and continuous cell lines by proinflammatory cytokines. J Immunol. 1988; 141: 1665–1669.[Abstract]

125. Williams AJ, Hale SL, Moffett JR, Dave JR, Elliott PJ, Adams J, Tortella FC. Delayed treatment with MLN519 reduces infarction and associated neurological deficit caused by focal ischemic brain injury in rats via antiinflammatory mechanisms involving nuclear factor-{kappa}B activation, gliosis, and leukocyte infiltration. J Cereb Blood Flow Metab. 2003; 23: 75–87.[CrossRef][Medline] [Order article via Infotrieve]

126. Lu X, Michaud C, Orlowski M. Heat shock protein-90 and the catalytic activities of the 20 S proteasome (multicatalytic proteinase complex). Arch Biochem Biophys. 2001; 387: 163–171.[CrossRef][Medline] [Order article via Infotrieve]

127. Conconi M, Petropoulos I, Emod I, Turlin E, Biville F, Friguet B. Protection from oxidative inactivation of the 20S proteasome by heat-shock protein 90. Biochem J. 1998; 333: 407–415.[Medline] [Order article via Infotrieve]

128. Zhang L, Zhang ZG, Zhang RL, Lu M, Adams J, Elliott PJ, Chopp M. Postischemic (6-Hour) treatment with recombinant human tissue plasminogen activator and proteasome inhibitor PS-519 reduces infarction in a rat model of embolic focal cerebral ischemia. Stroke. 2001; 32: 2926–2931.[Abstract/Free Full Text]

129. Keller JN, Huang FF, Zhu H, Yu J, Ho YS, Kindy TS. Oxidative stress-associated impairment of proteasome activity during ischemia-reperfusion injury. J Cereb Blood Flow Metab. 2000; 20: 1467–1473.[CrossRef][Medline] [Order article via Infotrieve]

130. Stephenson D, Yin T, Smalstig EB, Hsu MA, Panetta J, Little S, Clemens J. Transcription factor nuclear factor-{kappa}B is activated in neurons after focal cerebral ischemia. J Cereb Blood Flow Metab. 2000; 20: 592–603.[Medline] [Order article via Infotrieve]

131. Bailey CK, Andriola IF, Kampinga HH, Merry DE. Molecular chaperones enhance the degradation of expanded polyglutamine repeat androgen receptor in a cellular model of spinal and bulbar muscular atrophy. Hum Mol Genet. 2002; 11: 515–523.[Abstract/Free Full Text]

132. Halliwell B. Hypothesis: proteasomal dysfunction: a primary event in neurogeneration that leads to nitrative and oxidative stress and subsequent cell death. Ann N Y Acad Sci. 2002; 962: 182–194.[Medline] [Order article via Infotrieve]

133. Jenner P. Oxidative stress in Parkinson’s disease. Ann Neurol. 2003; 53: S26–S36.[CrossRef][Medline] [Order article via Infotrieve]

134. Mayer RJ. From neurodegeneration to neurohomeostasis: the role of ubiquitin. Drug News Perspect. 2003; 16: 103–108.[CrossRef][Medline] [Order article via Infotrieve]

135. Elliott PJ, Zollner TM, Boehncke WH. Proteasome inhibition: a new anti-inflammatory strategy. J Mol Med. 2003; 81: 235–245.[CrossRef][Medline] [Order article via Infotrieve]

136. Buchan AM, Li H, Blackburn B. Neuroprotection achieved with a novel proteasome inhibitor which blocks NF-{kappa}B activation. Neuroreport. 2000; 11: 427–430.[Medline] [Order article via Infotrieve]

137. Kikuchi S, Shinpo K, Takeuchi M, Tsuji S, Yabe I, Niino M, Tashiro K. Effect of geranylgeranylaceton on cellular damage induced by proteasome inhibition in cultured spinal neurons. J Neurosci Res. 2002; 69: 373–381.[CrossRef][Medline] [Order article via Infotrieve]

138. Keller JN, Huang FF, Dimayuga ER, Maragos WF. Dopamine induces proteasome inhibition in neural PC12 cell line. Free Radic Biol Med. 2000; 29: 1037–1042.[CrossRef][Medline] [Order article via Infotrieve]

139. Mattson MP, Camandola S. NF-{kappa}B in neuronal plasticity and neurodegenerative disorders. J Clin Invest. 2001; 107: 247–254.[Medline] [Order article via Infotrieve]

140. Howard EF, Chen Q, Cheng C, Carroll JE, Hess D. NF-{kappa}B is activated and ICAM-1 gene expression is upregulated during reoxygenation of human brain endothelial cells. Neurosci Lett. 1998; 248: 199–203.[CrossRef][Medline] [Order article via Infotrieve]

141. Kolev K, Skopal J, Simon L, Csonka E, Machovich R, Nagy Z. Matrix metalloproteinase-9 expression in post-hypoxic human brain capillary endothelial cells: H2O2 as a trigger and NF-{kappa}B as a signal transducer. Thromb Haemost. 2003; 90: 528–537.[Medline] [Order article via Infotrieve]




This article has been cited by other articles:


Home page
NeuroscientistHome page
R. Meller
The Role of the Ubiquitin Proteasome System in Ischemia and Ischemic Tolerance
Neuroscientist, June 1, 2009; 15(3): 243 - 260.
[Abstract] [PDF]


Home page
Cancer Res.Home page
A. Szokalska, M. Makowski, D. Nowis, G. M. Wilczynski, M. Kujawa, C. Wojcik, I. Mlynarczuk-Bialy, P. Salwa, J. Bil, S. Janowska, et al.
Proteasome Inhibition Potentiates Antitumor Effects of Photodynamic Therapy in Mice through Induction of Endoplasmic Reticulum Stress and Unfolded Protein Response
Cancer Res., May 15, 2009; 69(10): 4235 - 4243.
[Abstract] [Full Text] [PDF]


Home page
J Am Coll CardiolHome page
C. Di Filippo, R. Marfella, and M. D'Amico
Possible Dual Role of Ubiquitin-Proteasome System in the Atherosclerotic Plaque Progression
J. Am. Coll. Cardiol., October 14, 2008; 52(16): 1350 - 1351.
[Full Text] [PDF]


Home page
J. Biol. Chem.Home page
L. Yang, S. Wang, B. Sung, G. Lim, and J. Mao
Morphine Induces Ubiquitin-Proteasome Activity and Glutamate Transporter Degradation
J. Biol. Chem., August 1, 2008; 283(31): 21703 - 21713.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. ProteomicsHome page
O. Drews, R. Wildgruber, C. Zong, U. Sukop, M. Nissum, G. Weber, A. V. Gomes, and P. Ping
Mammalian Proteasome Subpopulations with Distinct Molecular Compositions and Proteolytic Activities
Mol. Cell. Proteomics, November 1, 2007; 6(11): 2021 - 2031.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
N. Yamamoto, H. Sawada, Y. Izumi, T. Kume, H. Katsuki, S. Shimohama, and A. Akaike
Proteasome Inhibition Induces Glutathione Synthesis and Protects Cells from Oxidative Stress: RELEVANCE TO PARKINSON DISEASE
J. Biol. Chem., February 16, 2007; 282(7): 4364 - 4372.
[Abstract] [Full Text] [PDF]


Home page
Reproductive SciencesHome page
C. E. Wood and D. Giroux
Expression of Nitric Oxide Synthase Isoforms in the Ovine Fetal Brain: Alteration by Hormonal and Hemodynamic Stimuli
Reproductive Sciences, July 1, 2006; 13(5): 329 - 337.
[Abstract] [PDF]


This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
35/6/1506    most recent
01.STR.0000126891.93919.4ev1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wojcik, C.
Right arrow Articles by Di Napoli, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wojcik, C.
Right arrow Articles by Di Napoli, M.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
Medline Plus Health Information
*Stroke
Related Collections
Right arrow Cell biology/structural biology
Right arrow Cell signalling/signal transduction
Right arrow Acute Cerebral Infarction
Right arrow Pathology of Stroke
Right arrow Other Stroke Treatment - Medical