This Article Abstract Full Text (PDF) All Versions of this Article: 36/6/1283    most recent 01.STR.0000166198.05439.f8v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Berthelemy-Okazaki, N. Articles by Cannon-Albright, L. A. Search for Related Content PubMed PubMed Citation Articles by Berthelemy-Okazaki, N. Articles by Cannon-Albright, L. A. Related Collections Cerebral Aneurysm, AVM, & Subarachnoid hemorrhage Genetics of Stroke Epidemiology

(Stroke. 2005;36:1283.)
© 2005 American Heart Association, Inc.

Research Reports

Examination of ELN as a Candidate Gene in the Utah Intracranial Aneurysm Pedigrees

Nicole Berthelemy-Okazaki, PhD; Yu Zhao, BS; Zhenglin Yang, MD; Nicola J. Camp, PhD; Jim Farnham, MS; Dennis Parker, PhD; Jay Tsuruda, MD; Joel MacDonald, MD; Kang Zhang, MD, PhD Lisa A. Cannon-Albright, PhD

From the Department of Medical Informatics (N.B.-O., N.J.C., J.F., D.P., L.A.C.-A.), University of Utah School of Medicine; the Weber State University (N.B.-O.), Ogden, Utah; the Department of Radiology (D.P.), University of Utah School of Medicine; Visiting Scholar in Department of Radiology (J.T.), University of Utah School of Medicine; the Department of Neurosurgery (J.M.), University of Utah School of Medicine; and Ophthalmology-Services (Y.Z., Z.Y., K.Z.), John A. Moran Eye Center, University of Utah School of Medicine.

Correspondence to Lisa Cannon-Albright, 391 Chipeta Way, Suite D, Salt Lake City, UT 84108. E-mail lisa{at}genepi.med.utah.edu

	Abstract

Top Abstract Introduction Materials and Methods Results Discussion References

 
Background and Purpose— A study of intracranial aneurysm (IA) sibpairs suggested association of an ELN haplotype with IA risk. Subsequent linkage analysis of the ELN region on chromosome 7q11 in high-risk Utah IA pedigrees significantly confirmed linkage between IA and the ELN region.

Methods— We have investigated the ELN gene as a potential candidate gene for IA in Utah pedigrees. One IA case from each pedigree, who shared an ELN region haplotype segregating in the pedigree, was screened for mutation. The promoter region, 34 exons, and the 3'UTR (UnTranslated Region) of the ELN gene were screened for variants using DHPLC.

Results— Variants were observed in the promoter region, exons 4 and 6, and the 3'UTR. Variants in exon 6 and in one 3'UTR position were unique to Utah. The remaining variants were absent in the controls. There was no evidence for segregation of the ELN variants found in IA cases with the hypothesized chromosome 7 haplotypes segregating in pedigrees.

Conclusion— Our analysis does not support ELN as the gene responsible for familial IA in the linked Utah IA pedigrees.

Key Words: aneurysm • genetics • intracranial aneurysm • pedigree

	Introduction

Top Abstract Introduction Materials and Methods Results Discussion References

 
Intracranial aneurysms (IAs) have a substantial genetic component.¹ In a genome-wide linkage search, Onda et al² found 3 candidate regions, with the strongest linkage near the elastin gene (ELN, OMIM 130160). Ruigrok et al³ found association between IA and ELN intron 5 and exon 22. These findings were not replicated in 2 European studies^4,5 or a Japanese population.⁶ In Farnham et al,⁷ we examined 13 IA pedigrees, and confirmed linkage of IA to a 7 cM region comprising the ELN gene. Here, we examine ELN as a candidate gene responsible for the Utah IA pedigrees.⁷

	Materials and Methods

Top Abstract Introduction Materials and Methods Results Discussion References

 
Our ELN mutation screening set consisted of 16 individuals: 1 affected individual from each of the 13 families⁷ was chosen to represent the haplotype on chromosome 7 cosegregating with IA. In 1 family, 2 different haplotypes were present, so 2 individuals were chosen, 1 to represent each haplotype. Two normal population controls with no history of IA were analyzed for comparison. Affected individuals had a confirmed medical diagnosis of IA as described in Farnham et al.⁷

The 16 genomic DNA samples were amplified by the polymerase chain reaction using primers corresponding to each of 34 ELN exons and their neighboring intronic regions, and to the promoter and 3'UTR regions described in Onda et al.² The polymerase chain reaction products were screened for mutations by DHPLC. Potential DNA variants were polymerase chain reaction-amplified and sequenced.⁸ Variants were considered possible candidates if the mutation was new to, or was significant in, the Japanese study. One affected and the oldest unaffected sibling was screened for a rapid and informal determination of segregation of the variant with IA.

	Results

Top Abstract Introduction Materials and Methods Results Discussion References

 
Variants were identified in the promoter region, introns 4 and 6, and the 3'UTR (Table). No further variation was found in the remaining 32 exons and exon/intron boundaries. For comparison, the frequencies of the Japanese variants are shown with the Utah results. A rare change at position –38 in the promoter region, present in the Japanese population, was not found in the Utah families. Variants in intron 6 and at position +549 in the 3'UTR were unique to the Utah cohort. None of these changes was present in the 2 controls.

View this table: [in this window] [in a new window]   Frequencies and Types of Variants Found in ELN in Utah Pedigrees

Because Onda et al² found no significant difference between controls and affected individuals for the variants in promoter (–972 or –1042), intron 4, and 3'UTR (+659), and we observed similar frequencies, no further segregation study was performed on these sites. The 3'UTR (+502) variant, which showed borderline significant difference (P=0.085) in the Japanese study, and the 2 novel variants identified in this study (3'UTR +549 and intron 6 –94) were examined by testing one affected and unaffected sibling in each pedigree for segregation with IA.

Neither affected nor unaffected siblings in the single pedigree with the 3'UTR G>A (+549) insertion carried informative haplotypes, so no inference could be made between this mutation and IA. The intron 6 variant was observed in 2 pedigrees (Table); however, the G>A (–94) mutation was found only in the unaffected sib of 1 pedigree, and was absent in the 2 individuals from the second pedigree. The 3'UTR (+502 ins A) variant was observed in 3 pedigrees. In the first pedigree, both the affected and the unaffected sibling also carried the variant. In the second pedigree, the 2 affected individuals shared both chromosome 7 haplotypes and the DNA change; the unaffected individual did not have the DNA variation. In the third pedigree, the A insertion was not observed in the affected sibling, and informative unaffected siblings were not available.

	Discussion

Top Abstract Introduction Materials and Methods Results Discussion References

 
We did not find compelling evidence of segregating mutations or polymorphisms in ELN explaining the IA aggregation observed in the Utah pedigrees. The absence of the mutations for both intron 6 and the 3'UTR variant in affected siblings rules out these mutations as the cause for IA. Two explanations might account for these results. The analysis in Farnham et al⁷ suggested a 7-cM region; it is possible that an unidentified gene in this region is responsible for the linkage evidence. Second, in this study, only exonic, promoter, and 3' UTRs of interest in Onda et al² were screened. Other regions of the ELN gene might be causal for IA.

Onda et al² and Ruigrock et al³ have found significant associations between IA and intron 20/intron 23, and intron 5/intron 22, respectively. Such results, in addition to Farnham et al,⁷ indicate that regulatory pathways for ELN synthesis and maintenance are likely to be involved in IA, rather than ELN structure. Additional sequencing of regulatory regions should be pursued.

	Acknowledgments

 
Partial support for all data sets within the Utah Population Database was provided by the University of Utah Huntsman Cancer Institute. Data collection was supported by: National Institutes of Health, National Institute of Neurological Disorders and Stroke grant R01 NS37737 (to L.A.C.A.), National Heart, Lung, and Blood Institute, R01HL48223 and HL53596 (to D.L.P.), and the National Eye Institute grants R01 EY14428 and R01 EY14448 (to K.Z.), the University of Utah General Clinical Research Center M01 RR00064, the Ruth and Milton Steinbach Fund; Ronald McDonald House Charities, and Macular Vision Research Foundation.

Received December 14, 2004; revision received March 1, 2005; accepted March 3, 2005.

	References

Top Abstract Introduction Materials and Methods Results Discussion References

 
1. Schievink WI. Genetics of intracranial aneurysms. Neurosurgery. 1997; 40: 651–662.[CrossRef][Medline] [Order article via Infotrieve]

2. Onda H, Kasuya H, Yoneyama T, Takakura K, Hori T, Takeda J, Nakajima T, Inoue I. Genome-wide-linkage and haplotype-association studies map intracranial aneurysm to chromosome 7q11. Am J Hum Genet. 2001; 69: 804–819.[CrossRef][Medline] [Order article via Infotrieve]

3. Ruigrock YM, Seitz U, Wolterink S, Rinkel GJE, Wijmenga C, Urban Z. Association of polymorphisms and haplotypes in the elastin gene in Dutch patients with sporadic aneurysmal subarachnoid hemorrhage. Stroke. 2004; 35: 2064–2068.[Abstract/Free Full Text]

4. Hofer A, Hermans M, Kubassek N, Sitzer M, Funke H, Stogbauer F, Ivaskevicius V, Oldenburg J, Burstscher J, Knopp U, Schoch B, Wanke I, Hubner F, Deinsberger W, Meyer B, Boecher-Schwarz H, Poewe W, Raabe A, Steinmetz H, Auberger G. Elastin polymorphism haplotype and intracranial aneurysms are not associated in central Europe. Stroke. 2003; 34: 1207–1211.[Abstract/Free Full Text]

5. Krex D, Konig IR, Ziegler A, Schackert, Schackert G. Extended single nucleotide polymorphism and haplotype analysis of the elastin gene in Caucasians with intracranial aneurysms provides evidence for racially/ethnically based differences. Cerebrovasc Dis. 2004; 18: 104–110.[CrossRef][Medline] [Order article via Infotrieve]

6. Yamada S, Utsunomiya M, Inouye K, Nozaki K, Miyamoto S, Hashomoto N, Takenaka K, Yoshinaga T, Koizumi A. Absence of linkage of familial intracranial aneurysms to 7q11 in highly aggregated Japanese families. Stroke. 2003; 34: 892–900.[Abstract/Free Full Text]

7. Farnham JM, Camp NJ, Neuhausen SL, Tsuruda J, Parker D, MacDonald J, Cannon-Albright L. Confirmation of chromosome 7q11 locus for predisposition to intracranial aneurysm. Hum Genet. 2004; 114: 250–255.[CrossRef][Medline] [Order article via Infotrieve]

8. Yang Z, Peachey NS, Moshfeghi DM, Thirumalaichary S, Chorich L, Shugart YY, Fan K, Zhang K. Mutations in the RPGR gene cause X-linked cone dystrophy. Hum. Mol Genet. 2002; 11: 605–611.

This article has been cited by other articles:

				T. Foroud, L. Sauerbeck, R. Brown, C. Anderson, D. Woo, D. Kleindorfer, M. L. Flaherty, R. Deka, R. Hornung, I. Meissner, et al. Genome Screen to Detect Linkage to Intracranial Aneurysm Susceptibility Genes: The Familial Intracranial Aneurysm (FIA) Study Stroke, May 1, 2008; 39(5): 1434 - 1440. [Abstract] [Full Text] [PDF]

				Y. M. Ruigrok and G. J.E. Rinkel Genetics of Intracranial Aneurysms Stroke, March 1, 2008; 39(3): 1049 - 1055. [Abstract] [Full Text] [PDF]

				Y. Mineharu, K. Inoue, S. Inoue, S. Yamada, K. Nozaki, N. Hashimoto, and A. Koizumi Model-Based Linkage Analyses Confirm Chromosome 19q13.3 as a Susceptibility Locus for Intracranial Aneurysm Stroke, April 1, 2007; 38(4): 1174 - 1178. [Abstract] [Full Text] [PDF]

				K. Inoue, Y. Mineharu, S. Inoue, S. Yamada, F. Matsuda, K. Nozaki, K. Takenaka, N. Hashimoto, and A. Koizumi Search on Chromosome 17 Centromere Reveals TNFRSF13B as a Susceptibility Gene for Intracranial Aneurysm: A Preliminary Study Circulation, April 25, 2006; 113(16): 2002 - 2010. [Abstract] [Full Text] [PDF]

This Article Abstract Full Text (PDF) All Versions of this Article: 36/6/1283    most recent 01.STR.0000166198.05439.f8v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Berthelemy-Okazaki, N. Articles by Cannon-Albright, L. A. Search for Related Content PubMed PubMed Citation Articles by Berthelemy-Okazaki, N. Articles by Cannon-Albright, L. A. Related Collections Cerebral Aneurysm, AVM, & Subarachnoid hemorrhage Genetics of Stroke Epidemiology