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(Stroke. 2005;36:1295.)
© 2005 American Heart Association, Inc.

Research Reports

Why Human Color Vision Cannot Reliably Detect Cerebrospinal Fluid Xanthochromia

From the Tavistock Intensive Care Unit (A.P.), The National Hospital for Neurology and Neurosurgery, and Department of Neuroimmunology, Institute of Neurology, Queen Square, London, UK; the Department of Neuroimmunology (G.K.), Institute of Neurology, Queen Square, London, UK; and the Colour and Vision Research Laboratories (T.L.S.; http://cvrl.ioo.ucl.ac.uk/index.htm), Institute of Ophthalmology, London, UK.

Correspondence to Dr Axel Petzold, the Department of Neuroimmunology, Institute of Neurology, Queen Square, London WC1N 3BG, UK. E-mail a.petzold{at}ion.ucl.ac.uk

	Abstract

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Background— Visual assessment of cerebrospinal fluid (CSF) for xanthochromia (yellow color) is practiced by the majority of laboratories worldwide as a means of diagnosing intracranical bleeds.

Methods— Colorimetric and spectrophotometric analysis of CSF samples for recognizing the presence of bilirubin either in low concentrations or in the presence of hemolysed blood.

Results— The experiments provide the physiological and colorimetric basis for abandoning visual assessment of CSF for xanthochromia.

Conclusion— We strongly recommend relying on spectrophotometry as the analytical method of choice.

Key Words: cerebrospinal fluid • intracerebral hemorrhage • subarachnoid hemorrhage

	Introduction

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Subarachnoid hemorrhage is one of the most striking conditions in medicine with potentially fatal outcome. The analysis of cerebrospinal fluid (CSF) is a crucial diagnostic tool. The sensitivity for detecting a bleed by CT decreases from 95% on day 1 to <10% 3 weeks after the event,¹ whereas the sensitivity of CSF analysis remains close to 100%.²

The presence of pigments in CSF alters its visual appearance. Oxyhemoglobin makes it appear red or orange, whereas bilirubin gives the yellow coloration of true xanthochromia. Oxyhemoglobin arises both from a traumatic tap and a true subarachnoid hemorrhage. Importantly, the conversion of oxyhemoglobin to bilirubin can only happen in vivo, allowing distinction between a true intracranial bleed and one caused by a traumatic tap. Here, we provide physiological evidence that the commonly practiced^3,4 visual assessment of CSF should be abandoned and replaced by spectrophotometry.

	Materials and Methods

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To simulate the conditions in which bilirubin may be observed, a set of experiments were designed. First, to reproduce contamination by oxyhemoglobin as it may occur with a traumatic tap, a series of tubes containing doubling dilutions of hemolysed blood (series A inset in Figure 1A) into CSF containing the same amount of bilirubin was prepared. Second, to determine the lowest concentration of bilirubin that could be confidently detected, we prepared a series of tubes containing doubling dilutions of bilirubin alone (series B inset in Figure 1A).

View larger version (24K): [in this window] [in a new window]   A, A section of the CIE 1931 chromaticity diagram (the full diagram is shown in the lower left-hand corner) displaying the x–y chromaticity coordinates of 14 samples simulating a traumatic tap derived by double diluting hemolyzed blood into xanthochromic CSF sample B1 (series A, circles) and 8 samples derived from a double dilution of xanthochromic CSF in H2O (series B, triangles). The coordinates have been calculated with respect to CIE illuminant D65 (white cross). For reference, the x–y chromaticity coordinates and wavelengths of the spectral colors from 550 nm (yellow green) to 620 nm (red) are also indicated. The dominant wavelengths (turquoise lines) of samples B1 to B8 and sample A1 are shown. The region corresponding to the color category "pure yellow," which ranges between 575 and 580 nm, is indicated in pale gray (see summary table in reference 6). The photographic insets show the series A and B samples illuminated by cool white fluorescent light. B, The x–y chromaticity coordinates of the 22 samples from series A (circles) and B (triangles) calculated for CIE illuminant A (white cross). The dominant wavelengths (turquoise lines) of samples B1 to B8 and sample A1 are shown. The photographic inset shows series A and B illuminated by a tungsten halogen source.

All tubes were examined visually for xanthochromia, in normal daylight or cool white fluorescent light, the typical viewing conditions, by 11 analysts, comprising clinical scientists, biomedical scientists, or clinical neurology staff within the Department of Neuroimmunology at National Hospital for Neurology and Neurosurgery. The tubes were presented in a random order and the analysts were naive to their actual concentrations.

Once the visual assessments were complete, all samples were scanned between 350 and 740 nm using an Ultrospec 4300 pro (Amersham Biosciences). The same analysts were then asked to indicate whether bilirubin was present or absent in the scan. The proportions of subjects finding a positive result by visual or spectroscopic assessment were compared using a ² test.

Finally, the x–y chromaticity coordinates of the spectrophotometric scans were calculated according to the standard procedures of specification for visual assessment established by the Commission Internationale de l’Eclairage (CIE, the International Commission on Illumination). This involves multiplying the spectral transmittances of the samples, converted from their optical densities, by the spectral concentration of the radiant power of the source illuminating them and then multiplying the product by each of the 3 color-matching functions, which define the CIE standard colorimetric observer.⁵ The resulting x–y chromaticity coordinates of the samples can then be plotted in the CIE 1931 chromaticity diagram for the standard 2° field of view (Figure 1A and 1B), and their dominant wavelengths, which correspond to hue, and excitation purities, which correspond to saturation, can be geometrically calculated (see explanations in the Table and Figure legends and values in Table). The calculations were made for 2 CIE illumination or lighting standards: "D65," which equates to average daylight; and "A," which is for tungsten light.

	Results

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Samples in series A, when calculated for viewing by standard illuminant D65, varied in their dominant wavelength from 572 nm (which falls near the hue category "pure yellow"⁶) to 615 nm (red). They also varied in their excitation purity from 97.9% (highly saturated) to 34.1% (moderately saturated). In contrast, the samples in series B all had the same dominant wavelength, 572 nm (yellow), but differed in their excitation purity from 0.62 (very desaturated) to 36.6% (Table). The optical density for bilirubin (450 to 460 nm) ranged from 3.5 to 0.36 for samples A1 to A14 and from 3.2 to 0.002 for samples B1 to B8.

A significantly higher proportion of the analysts detected traces of bilirubin spectrophotometrically than visually, both when the xanthochromic CSF samples were contaminated by the presence of hemolyzed blood (series A) and when they were desaturated (series B). In series A, visual detection failed for CSF samples with dominant wavelengths >574 nm (samples A1 to A7), most of which fall considerably outside the color category "pure yellow." In series B, bilirubin could not be reliably detected in CSF specimens with excitation purity levels <2.4% (samples B5 to B8). In contrast, in both series A and B, bilirubin could be reliably detected in all the samples by examining the spectrophotometric scans.

This study confirms that spectrophotometry is superior to color vision for analyzing CSF samples for the presence of bilirubin.⁷ Most critical CSF samples are either contaminated by oxyhemoglobin or have only low levels of bilirubin. Under such conditions, detection of xanthochromia becomes unreliable, especially when viewed under incandescent lighting or a tungsten desk lamp (corresponding to CIE standard illuminant A). A lower proportion of the assessors were able to detect xanthochromia for samples B4 (7/11, ²=4.88, P<0.05), B5 (3/11, ²=9.21, P<0.01), and B6 (0/11, ²=6.47, P=0.01; see insets in Figure 1B) under tungsten light than under daylight conditions. Colorimetric analysis revealed that all of the samples now fell completely outside the "pure yellow" category. This condition represents a "worst-case scenario," such as may be encountered during a night on-call.

	Discussion

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The implications of these findings can be judged from our previous analysis of spectrophotometric scans of CSF samples, which did not appear yellow in almost 80% of the cases encountered at the National Hospital for Neurology and Neurosurgery.⁸

Approximately 80% of all CSF samples with significant amounts of bilirubin appear rather "red" than "yellow,"⁸ but 99.7% of >3500 laboratories participating in 2 recent American surveys^3,4 still assess samples by color vision. The observations presented here provide a physiological basis for abandoning the visual assessment of CSF for xanthochromia and rely on spectrophotometry instead.⁹

	Acknowledgments

 
We thank Robert Ludlow for excellent photographic support, Miles Chapman for discussion and advice on the practical experiments, and E. J. Thompson, G. T. Plant, and M. Smith for comments on the manuscript.

Received January 7, 2005; accepted February 25, 2005.

	References

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1. van Gijn J, van Dongen KJ. The time course of aneurysmal haemorrhage on computed tomograms. Neuroradiology. 1982; 23: 153–156.[Medline] [Order article via Infotrieve]

2. Vermeulen M, Hasan D, et al. The time course of aneurysmal haemorrhage on computed tomograms. J Neurol Neurosurg Psych. 1989; 52: 826–828.[Abstract/Free Full Text]

3. Edlow JA, Bruner KS, Horowitz GL. Xanthochromia. Arch Pathol Lab Med. 2002; 126: 413–415.[Medline] [Order article via Infotrieve]

4. Judge B. Laboratory Analysis of Xanthochromia in Patients With Suspected Subarachnoidal Hemorrhage: A National Survey. Philadelphia: Scientific Assembly, American College of Emergency Physicians; 2000.

5. Wyszecki G, Stiles WS. Color Science: Concepts and Methods, Quantitative Data and Formulae, 2nd ed. New York: John Wiley & Sons; 2000.

6. Petzold A, Sharpe LT. Hue memory and discrimination in young children. Vision Res. 1998; 38: 3759–3772.[CrossRef][Medline] [Order article via Infotrieve]

7. Marden NA, Thomas PH, Stansbie D. Is the naked eye as sensitive as the spectrophotometer for detecting xanthochromia in cerebrovascular disease? In: Martin SM, ed. Proceedings of the National Meeting, 2001 April 30–May 4, London. London: Association of Clinical Biochemists, 2001: 53.

8. Petzold A, Keir G, Sharpe LT. Spectralphotometry for xanthochromia. N Eng J Med. 2004; 351: 1695–1696.[Free Full Text]

9. UK NEQAS For Immunochemistry Working Group. National guidelines for analysis of cerebrospinal fluid for bilirubin in suspected subarachnoid haemorrhage. Ann Clin Biochem. 2003; 40: 481–488.[CrossRef][Medline] [Order article via Infotrieve]

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