This Article Abstract Full Text (PDF) All Versions of this Article: 37/8/2134    most recent 01.STR.0000231875.96714.b1v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ito, U. Articles by Oyanagi, K. Search for Related Content PubMed PubMed Citation Articles by Ito, U. Articles by Oyanagi, K. Related Collections Animal models of human disease Apoptosis Exercise/exercise testing/rehabilitation Acute Cerebral Infarction

(Stroke. 2006;37:2134.)
© 2006 American Heart Association, Inc.

Original Contributions

Temporal Profiles of Axon Terminals, Synapses and Spines in the Ischemic Penumbra of the Cerebral Cortex

Ultrastructure of Neuronal Remodeling

Umeo Ito, MD, PhD, FAHA; Toshihiko Kuroiwa, MD, PhD; Jun Nagasao, DVM; Emiko Kawakami, BS Kiyomitsu Oyanagi, MD, PhD

From the Department of Neuropathology (U.I., J.N., E.K., K.O.), Tokyo Metropolitan Institute for Neuroscience, Tokyo, Japan; and the Department of Neuropathology (T.K.), Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan.

Correspondence to Umeo Ito, MD, PhD, FAHA, Department of Neuropathology, Tokyo Metropolitan Institute for Neuroscience, Tokyo, 2-6, Musashidai, Fuchu-shi, Tokyo 183-8526, Japan. E-mail umeo-ito{at}nn.iij4u.or.jp

	Abstract

Top Abstract Introduction Materials and Methods Results Discussion References

 
Background and Purpose— Because the recovery process of axon terminals, synapses, and spine-dendrites in the ischemic penumbra of the cerebral cortex is obscure, we studied the temporal profile of these structures up to 12 weeks after the ischemic insult, using a gerbil model.

Methods— Stroke-positive animals were selected according to their stroke index score during the first 10-minute left carotid occlusion done twice with 5-hour interval. The animals were euthanized at various times after the second ischemic insult. Ultra-thin sections including the 2nd to 4th cortical layers were obtained from the neocortex coronally sectioned at the infundibular level, in which the penumbra appeared. We counted the number of synapses, spines and multiple synapse boutons, measured neurite thickness, and determined the percent volume of the axon terminals and spines by Weibel point counting method.

Results— The number of synapses, synaptic vesicles and spines and the total percent volume of the axon terminals and spines decreased until the 4th day. From 1 to 12 weeks after the ischemic insult, these values increased to or exceeded the control ones, and neuritic thickening and increase in number of multiple synapse boutons occurred.

Conclusions— In the ischemic penumbra, the above structures degenerated, with a reduction in their number and size, until 4 days and then recovered from 1 to 12 weeks after the ischemic insult.

Key Words: axon terminal • dendrites • spine • synapses • transient cerebral ischemia

	Introduction

Top Abstract Introduction Materials and Methods Results Discussion References

 
Cerebral infarction develops rapidly after a large ischemic insult. Earlier we developed a model of temporary ischemia in which a focal infarction surrounded by a large penumbra was produced in the cerebral cortex of Mongolian gerbils by giving a threshold amount of ischemic insult to induce focal infarction.^1,2

Concerning the neuronal recovery, recent findings have revealed that synapses and their networks express a high degree of functional and structural plasticity.³ Ultrastructural changes in the postsynaptic density in hippocampal CA-1 were investigated after temporary ischemia.^4–6 Degenerated boutons and multiple synapse boutons (MSBs) in this region were investigated by electron microscopy (EM) after temporary ischemia,^7–9 as well as after temporary hypoxia/hypoglycemia in hippocampal slices.¹⁰ Changes in spines and dendrites were studied by time-lapse microscopy after temporary anoxia/hypoglycemia in cell culture,^11,12 by light microscopy (LMS) of Golgi stain-impregnated sections of the 3rd to 5th cortical layers of the cerebral cortex after temporary ischemia,¹³ and by EM after temporary hypoxia/glycemia in hippocampal slice.¹⁰ Changes in CA-1 dendrites after temporary ischemia were investigated by light microscopy of horseradish peroxidase-injected specimens,¹⁴ by EM after temporary ischemia in CA-1,^15,16 and by EM of Golgi stain-impregnated cerebral cortex after temporary ischemia for 20 minutes.¹⁷

Almost all of the above studies were performed in connection with delayed ischemia-induced injury to CA-1 neurons, and observations were made during a short period after ischemia. However, clinically, most patients show gradual recovery from behavioral dysfunctions after a stroke. The long-term integrated profile of axon terminals, synapses, spines, and dendrites during the recovery stage after an ischemic insult has remained obscure, especially in the ischemic penumbra of the cerebral cortex.

Because no functional recovery is anticipated in the infarction itself,^18–20 we aimed to elucidate neuronal remodeling process in the ischemic penumbra in which neuronal death progresses in a disseminated fashion,^2,18,20 by focusing on the temporal profiles of axon terminals, synapses, spines, and neurites.

	Materials and Methods

Top Abstract Introduction Materials and Methods Results Discussion References

 
Under anesthesia with 2% halothane, 70% nitrous oxide, and 30% oxygen, the left carotid artery of adult male Mongolian gerbils (60 to 80 g) was twice occluded with a Heifetz aneurismal clip for 10 minutes each time, with a 5-hour interval between the 2 occlusions, anesthesia was discontinued immediately after each cervical surgery, the animals soon became awake and moved spontaneously.

Ischemia-positive animals registering >13 points were selected based on the stroke index score determined during the first occlusion.²¹ The gerbils were euthanized at various times, ie, at 5, 12, 24, 48-hour, 4 days, and 1, 5, 8, and 12 weeks after the ischemic insult. Anesthetization was followed by intracardiac perfusion with diluted fixative (1% paraformaldehyde, 1.25% glutaraldehyde in 0.1 mol/L cacodylate buffer) for 5 minutes, followed by perfusion with concentrated fixative (4% paraformaldehyde, 5% glutaraldehyde in 0.1 mol/L cacodylate buffer) for 20 minutes for EM (3 animals in each time group), or with 10% phosphate-buffered formaldehyde fixative for 30 minutes for LMS (5 animals in each time group).

In this model, after the restoration of blood flow, only ischemic penumbra with progressing disseminated selective neuronal necrosis (DSNN) appeared in the coronal face sectioned at the infundibular level (Face B) and focal infarction evolved among the DSNN in the coronal face sectioned at the chiasm (Face A). Ultra-thin sections including the 2nd to 4th cortical layers were prepared from the left cerebral cortex at the mid-point between the interhemispheric and rhinal fissures on Face B, penumbra >1 mm caudal to infarction edge. The sections were double stained with uranyl acetate and lead solution, and observed with an electron microscope (H9000, Hitachi). Paraffin sections of both faces were separately stained with hematoxylin-eosin (HE) or periodic acid fuchsin Schiff (PAS) or by Bodian silver impregnation or used for immunohistochemical detection of glial fibrillary acidic protein.

Placing 1.0 cmx1.0 cm quadratic lattices of points on 5000x2.67 times enlarged EM photographs, we measured the number of synapses (synapses: consist of the pre- and postsynaptic densities associated with their cytoplasmic faces, and the synaptic cleft between them) and spines (spines: an ovoid bulb that is filled with a fluffy material and connected to dendrite directly or by a stalk) in the neuropil in a 100-cm² area (56 µm², by real size) by examining 1800±364 cm² in the neuropil of 3 animals in each time group. We determined the percent volume of the axon terminals (axon terminals: presynaptic expansion of the axon that contains synaptic vesicles and mitochondria) and spines by using the point counting method²² in which the number of intersecting points touched by the axon terminals and/or spines were counted among 1000 to 15 000 points (counting number varied according to the equation of the relative error for different volumetric proportions) of the quadratic lattice in each time group. We measured the thickness of 194±38 neurites (neurites: axons and dendrites those contain microtubules, neurofilaments and mitochondria; differentiation between them in transverse section is often difficult, especially in small ones) in each time group, as the maximal diameter perpendicular to their neurofilaments and/or microtubules. We also measured the percentage of MSBs by counting 327±38 synapses in each time group, all on the same EM pictures. The statistical differences between each of the time groups were analyzed by ANOVA, followed by Bonferroni-Dunn test. All data in the Table and Figure 6 were presented as average±SEM and a statistical difference was accepted at P<0.05 level.

View this table: [in this window] [in a new window]   Percent of MSBs Among Synapses

	Results

Top Abstract Introduction Materials and Methods Results Discussion References

 
In the ischemic penumbra of the cerebral cortex in Face B, eosinophilic ischemic neurons (HE staining) appeared in disseminated fashion among the normal-looking neurons in the 2nd to 6th cortical layers by LMS, around 5 hours after the ischemic insult. Some of these eosinophilic cell bodies became remarkably shrunken and died during the period of 12 to 48 hours, which indicates DSNN (Figure 1A). The eosinophilic ischemic neurons were found by EM to be disseminated electron-dense dark neurons that increased in number during the period of 12 to 48 hours after the ischemic insult.

View larger version (131K): [in this window] [in a new window]   Figure 1. Light-microscopy of 2nd to 4th cortical layers of the cerebral cortex in Face B. A, Twenty-four hours after the ischemic insult, some of the eosinophilic ischemic neurons show marked shrinkage compared with the more normal-looking neurons, indicating disseminated selective neuronal necrosis. These abnormal neurons increased in number until day 2 to 3 postischemia (HE, Bar 31.3 µm). B, Eight weeks after the ischemic insult. The eosinophilic ghost cells of faintly formed cell bodies are reduced in size and are found in the 3rd cortical layer (PAS, Bar 12.5 µm).

From 4 days until 8 weeks, these condensed electron-dense dark neurons became fragmented into an accumulation of electron-dense granular fragments, which were observed by LMS as eosinophilic ghost cells of faintly formed cell bodies by HE and PAS staining (Figure 1B). During 2 to 12 weeks, these eosinophilic ghost cells accumulated in the 3rd and occasionally in the 5th cortical layer decreasing in size because of loss of their periphery (Figure 1B). In Face A, focal infarction evolved and developed among the DSNN from 12 hours to 4 days.

From 4 days to 12 weeks after the ischemic insult, the axon terminals of the surviving neurons were found being attached to the peripherally located electron-dense granular fragments and dendritic portions of the shrunken dark neurons. Some of these terminals appeared to have pinched off pieces of the dead neurons, and they bore a crust of the electron-dense granular fragments of the dead neurons (Figure 2A). Other axon terminals were found attached to the electron-dense thick neurites of dead neurons (Figure 2B). Some of the fragment-encrusted axon terminals were occasionally observed to have synapsed with the spines and neurites of the surviving neurons (Figure 3A). Some axons connected to the dying neurons showed globular and abnormal distensions of their terminals as seen by silver impregnation (Figure 4A). These structures appeared as degenerated axon by EM. The amplified degenerated axon contained degenerated mitochondria, laminated dense bodies, and irregularly located neurofilaments and microtubules; the degenerated axon occasionally made synapses onto adjacent structures (inset of Figure 4A).

View larger version (154K): [in this window] [in a new window]   Figure 2. Electron microscopy of 3rd layer of the cerebral cortex in Face B, 1 week after the ischemic insult. A, The axon terminals of the surviving neurons make contact with the periphery of the accumulated fragments of electron-dense granules of dead neurons (DN). Some of them appeared to have pinched off pieces of the dead neurons, and are encrusted by the electron-dense granular fragments of the dead neurons (arrows). Bar 2.3 µm. B, Some axon terminals are found attached to the electron dense thick neurites of dead neurons (arrows). Bar 2.6 µm.

View larger version (156K): [in this window] [in a new window]   Figure 3. A, Electron microscopy of the 3rd cortical layer in Face B, 1 week after the ischemic insult. Some axon terminals encrusted by the electron-dense granular fragments of the dead neurons are occasionally observed to have made synapses with the spines and neurites of the surviving neurons (arrow). Bar 3.1 µm. B, Electron microscopy of 3rd cortical layer of the cerebral cortex in Face B, 12 weeks after the ischemic insult. MSBs with >2 spines synapsed (arrows) to 1 axon terminal (a). A widened spine(s) with multiple synapses on axon terminal (a) is seen. Bar 3.2 µm.

View larger version (156K): [in this window] [in a new window]   Figure 4. A, Light-microscopy. Four days after the ischemic insult, some axons attached to dying neurons show globular and abnormal distension of their terminals (arrow-heads). Bar 8.9 µm. Bodian’s silver impregnation. Inset: EM observation of the distended degenerated axon 3 weeks after the ischemic insult. It contains degenerated mitochondria, laminated dense bodies, and irregularly located neurofilaments and microtubules and has synapses along its wall (arrows). Bar 1.3 µm. B, Electron microscopy of the 3rd layer of the cerebral cortex in Face B, 2 weeks after the ischemic insult. These amplified degenerated axons (arrows) are observed around accumulations of the fragmented electron-dense granular pieces of the dead neurons (DN). Bar 0.6 µm.

Such axons were often observed around the accumulations of the fragmented electron-dense granular pieces of the dead neurons (Figure 4B).

By 12 weeks after the ischemic insult, neuritic shafts and their branches were remarkably thickened (Figure 5B) compared with those at day 4 (Figure 5A), and they made synapses with voluminously enlarged and occasionally sprouting polygonal axons terminals filled with synaptic vesicles (Figure 5B). MSBs²³ of the axons terminals (Figure 3B) increased in frequency compared with those of the control animals (Table).

View larger version (171K): [in this window] [in a new window]   Figure 5. Electron microscopy of the 3rd layer of the cerebral cortex in Face B. A, Four days after the ischemic insult. The volume of axon-terminals and spines has decreased with a decrease in frequency of synaptic vesicles, especially those close to the synapse (arrows). The thickness of degenerated neurites has decreased slightly compared with that of the control (arrowheads). Bar 1.5 µm. B, Twelve weeks after the ischemic insult. The neuritic shafts and their branches are remarkably thickened (arrowheads) and make synapses with voluminously enlarged and occasionally sprouting polygonal axon terminals filled with synaptic vesicles (arrows). Bar 1.5 µm.

The percent volume of the total axon terminals (Figure 6A) and spines (Figure 6B) in the neuropil decreased drastically to 30.9% and 24.8%, respectively, of the control value at that time after the ischemic insult, with a decrease in frequency of synaptic vesicles especially those close to the synapses (Figure 5A). The number of synapses also decreased to 73.5% of the control value at 4 days, after a temporary increase up to 135% at 5 hours after the ischemic insult (Figure 6A). The number of spines also decreased to 35.8% of the control value (Figure 6B), by 4 days. From 1 to 12 weeks after the ischemic insult, the percent volume of the total axon terminals (Figure 6A) and the percent volume of the total spines (Figure 6B) increased, being 162.8% and 86.7%, respectively, of the control value at 12 weeks. The number of synapses (Figure 6A) and spines (Figure 6B) also rose, becoming 113.2% and 91.9%, respectively, of it at that time.

View larger version (50K): [in this window] [in a new window]   Figure 6. A, Time course of percent volume of axon terminals and number of synapses in the neuropil at various time after ischemic insult. B, Time course of percent volume and number of spines in the neuropil at various time post ischemia. C, Time course by scatter graph (upper) and average thickness (lower) of neurites in the neuropil after the ischemic insult. aCompared with control; bcompared with 4 days; *P<0.001, P<0.05.

The average thickness of neurites in the neuropil of the control animals were 0.607 µm. This value was unchanged at 0.587 µm at day 4, 0.604 µm at 1 week, and 0.665 µm at 8 weeks, and increased to 0.934 µm at 12 weeks after the ischemic insult (Figure 6C).

	Discussion

Top Abstract Introduction Materials and Methods Results Discussion References

 
In the neuropils of the ischemic penumbra in the cerebral cortex, we found a marked decrease in the number of the synapses and volume of the axon terminals from 5 hours to 4 days after the ischemic insult, along with a decrease in the number of synaptic vesicles. These changes may be attributed to a demolished synaptic neurotransmission attributable to calcium-dependent neuronal hyperexcitation^4–6 and could be reduced by NMDA (N-methyl-D-asparate) receptor antagonists as was reported in a morphological study recording excitatory postsynaptic potential from hippocampal slice cultures subjected to brief anoxia-hypoglycemia.¹⁰

Almost in accordance with our present study, the LMS study of Golgi silver impregnated spine and dendrites showed that the number of spines and thickness of dendrites decreased maximally in 4 to 7 days after the temporary ischemia and recovered around 5 weeks in the 2nd to 3rd cortical layers of the rat cerebral cortex.¹³ Earlier studies on cultured neurons showed a decreased spine number and segmental dendritic beading after temporary hypoxia/hypoglycemia followed by recovery,^11,12 and a LMS study using horseradish protein injection showed beading of dendrites in the CA-1 of the hippocampus after temporary ischemia.¹⁴ Also an EM study on the CA-1 showed degeneration and shrinkage of dendrite around 3 to 4 days after temporary ischemia.^15–17 In the present study, we found that the neurites degenerated around 4 days and that their thickness increased, in association with the recovery to normal of the number and percent volume of spines, at 12 weeks after the insult.

From 1 to 12 weeks after the ischemic insult, we found that the synaptic number increased gradually in association with an increase in the volume of axon terminals showing sprouting. The present study also showed an increase in the number of the MSBs from 8 to 12 weeks after the ischemic insult, which increase was associated with one in the number and volume of axon terminals and spines. The MSBs represent 2 independent dendritic spines contacting the same axon terminal.²³ One spine branched to make synapses at >2 portions of 1 axon terminal is considered to facilitate neurotransmission.¹⁰ In another study, there was an increase in the number of MSBs in CA-1, paralleling the marked increase in the number of synaptic vesicles after temporary ischemia⁷ and after temporary hypoxia/hypoglycemia in hippocampal slices.¹⁰

From 4 days to 12 weeks after the ischemic insult, some axons attached to the dying neurons showed an abnormal distension of their terminals, which contained degenerated mitochondria, laminated dense bodies, and irregularly located neurofilaments and microtubules (degenerated axon).^8,9 They were frequently observed around accumulations of the electron-dense granular fragments of the dead neurons.

Some axon terminals encrusted with the electron-dense granular fragments of the dead neurons became connected to the spines and neurites of the surviving neurons. These axon terminals, previously attached to the dying and/or dead neurons, seemed to become newly connected to the spines and to the thickened dendrites of the surviving neurons associated with synaptogenesis in the neuropil.²⁴ However, it could also be that some of these axon terminals were originally contacting more than one dendrites or spines.

Clinically, most stroke survivors show recovery from behavioral dysfunctions. The short-term recovery may be attributable to the resolution of brain edema. A more gradual recovery, promoted by exercise for rehabilitation, may be attributed to the anatomical and functional recovery of the penumbra. Stroemer²⁴ reported behavioral recovery after neocortical infarction in rats, which recovery was associated with neuronal sprouting followed by synapto-genesis, as demonstrated by immunohistochemical staining for GAP-43, a growth-associated protein expressed on axonal growth cones, and for synaptophysin. Functional remodeling of the cerebral cortex remote from the infarction was detected by intracortical microstimulation mapping of the hand of the squirrel monkey.^25,26

Activation of the complement system was shown to promote neuronal survival and tissue remodeling.²⁷ Postischemic treatment with brain-derived neurotrophic factor and physically exercised animals had better functional motor recovery, attributable to induction of widespread neuronal remodeling, as demonstrated by MAP1B and synaptophysin expression.¹⁹ Clinical introduction of novel agents and functional methods to promote synaptogenesis and neuronal networks in the iscehmic penumbra, is highly anticipated.

Summary
In the penumbra around a focal infarction of the cerebral cortex, synapses, synaptic vesicles, axon terminals, spines degenerated, with a reduction in their number and size, until 4 days and then recovered from 1 to 12 weeks after the ischemic insult.

	Acknowledgments

 
Source of Funding

This work was supported in part by grant from the Japanese Ministry of Health, Labor and Welfare, Research on Psychiatric and Neurological Diseases (H16-kokoro-017 to K.O.)

Received February 15, 2006; revision received May 1, 2006; accepted May 24, 2006.

	References

Top Abstract Introduction Materials and Methods Results Discussion References

 
1. Hanyu S, Ito U, Hakamata Y, Nakano I. Topographical analysis of cortical neuronal loss associated with disseminated selective neuronal necrosis and infarction after repeated ischemia. Brain Res. 1997; 767: 154–157.[CrossRef][Medline] [Order article via Infotrieve]

2. Ito U, Hanyu S, Hakamata Y, Kuroiwa T, Yoshida M. Features and threshold of infarct development in ischemic maturation phenomenon. In: Ito U, Kirino T, Kuroiwa T, Klatzo I 2nd, eds. Maturation Phenomenon in Cerebral Ischemia. Berlin, Heidelberg: Springer-Verlag; 1997: 115–121.

3. Luscher C, Nicoll RA, Malenka RC, Muller D. Synaptic plasticity and dynamic modulation of the postsynaptic membrane. Nat Neurosci. 2000; 3: 545–550.[CrossRef][Medline] [Order article via Infotrieve]

4. von Lubitz DK, Diemer NH. Cerebral ischemia in the rat: ultrastructural and morphometric analysis of synapses in stratum radiatum of the hippocampal CA-1 region. Acta Neuropathol (Berl). 1983; 61: 52–60.[CrossRef][Medline] [Order article via Infotrieve]

5. Martone ME, Jones YZ, Young SJ, Ellisman MH, Zivin JA, Hu BR. Modification of postsynaptic densities after transient cerebral ischemia: a quantitative and three-dimensional ultrastructural study. J Neurosci. 1999; 19: 1988–1997.[Abstract/Free Full Text]

6. Liu CL, Martone ME, Hu BR. Protein ubiquitination in postsynaptic densities after transient cerebral ischemia. J Cereb Blood Flow Metab. 2004; 24: 1219–1225.[CrossRef][Medline] [Order article via Infotrieve]

7. Briones TL, Suh E, Jozsa L, Hattar H, Chai J, Wadowska M. Behaviorally-induced ultrastructural plasticity in the hippocampal region after cerebral ischemia. Brain Res. 2004; 997: 137–146.[CrossRef][Medline] [Order article via Infotrieve]

8. Crain BJ, Evenson DA, Polsky K, Nadler JV. Electron microscopic study of the gerbil dentate gyrus after transient forebrain ischemia. Acta Neuropathol (Berl). 1990; 79: 409–417.[CrossRef][Medline] [Order article via Infotrieve]

9. Ishimaru H, Casamenti F, Ueda K, Maruyama Y, Pepeu G. Changes in presynaptic proteins, SNAP-25 and synaptophysin, in the hippocampal CA1 area in ischemic gerbils. Brain Res. 2001; 903: 94–101.[CrossRef][Medline] [Order article via Infotrieve]

10. Jourdain P, Nikonenko I, Alberi S, Muller D. Remodeling of hippocampal synaptic networks by a brief anoxia-hypoglycemia. J Neurosci. 2002; 22: 3108–3116.[Abstract/Free Full Text]

11. Park JS, Bateman MC, Goldberg MP. Rapid alterations in dendrite morphology during sublethal hypoxia or glutamate receptor activation. Neurobiol Dis. 1996; 3: 215–227.[CrossRef][Medline] [Order article via Infotrieve]

12. Hasbani MJ, Schlief ML, Fisher DA, Goldberg MP. Dendritic spines lost during glutamate receptor activation reemerge at original sites of synaptic contact. J Neurosci. 2001; 21: 2393–2403.[Abstract/Free Full Text]

13. Akulinin VA, Stepanov SS, Semchenko VV, Belichenko PV. Dendritic changes of the pyramidal neurons in layer V of sensory-motor cortex of the rat brain during the postresuscitation period. Resuscitation. 1997; 35: 157–164.[CrossRef][Medline] [Order article via Infotrieve]

14. Hori N, Carpenter DO. Functional and morphological changes induced by transient in vivo ischemia. Exp Neurol. 1994; 129: 279–289.[CrossRef][Medline] [Order article via Infotrieve]

15. Petito CK, Pulsinelli WA. Delayed neuronal recovery and neuronal death in rat hippocampus following severe cerebral ischemia: possible relationship to abnormalities in neuronal processes. J Cereb Blood Flow Metab. 1984; 4: 194–205.[Medline] [Order article via Infotrieve]

16. Yamamoto K, Hayakawa T, Mogami H, Akai F, Yanagihara T. Ultra-structural investigation of the CA1 region of the hippocampus after transient cerebral ischemia in gerbils. Acta Neuropathol (Berl). 1990; 80: 487–492.[CrossRef][Medline] [Order article via Infotrieve]

17. Tomimoto H, Yanagihara T. Golgi electron microscopic study of the cerebral cortex after transient cerebral ischemia and reperfusion in the gerbil. Neuroscience. 1994; 63: 957–967.[CrossRef][Medline] [Order article via Infotrieve]

18. Graham DI, Lantos PL. In: Greenfield’s Neuropathology, 7th ed. Arnold, London; 2002: 233–355.

19. Schabitz WR, Berger C, Kollmar R, Seitz M, Tanay E, Kiessling M, Schwab S, Sommer C. Effect of brain-derived neurotrophic factor treatment and forced arm use on functional motor recovery after small cortical ischemia. Stroke. 2004; 35: 992–997.[Abstract/Free Full Text]

20. Ito U, Spatz M, Walker J Jr, Klatzo I. Experimental cerebral ischemia in mongolian gerbils. I. Light microscopic observations. Acta Neuropathol Berl. 1975; 32: 209–223.[CrossRef][Medline] [Order article via Infotrieve]

21. Ohno K, Ito U, Inaba Y. Regional cerebral blood flow and stroke index after left carotid artery ligation in the conscious gerbil. Brain Res. 1984; 297: 151–157.[CrossRef][Medline] [Order article via Infotrieve]

22. Weibel ER. Morphometry of the human lung. Springeer-Verlag, Berlin, Gowttingen, Heidelberg; 1963: 19–22.

23. Sorra KE, Harris KM. Occurrence and three-dimensional structure of multiple synapses between individual radiatum axons and their target pyramidal cells in hippocampal area CA1. J Neurosci. 1993; 13: 3736–3748.[Abstract]

24. Stroemer RP, Kent TA, Hulsebosch CE. Neocortical neural sprouting, synaptogenesis, and behavioral recovery after neocortical infarction in rats. Stroke. 1995; 26: 2135–2144.[Abstract/Free Full Text]

25. Frost SB, Barbay S, Friel KM, Plautz EJ, Nudo RJ. Reorganization of remote cortical regions after ischemic brain injury: a potential substrate for stroke recovery. J Neurophysiol. 2003; 89: 3205–3214.[Abstract/Free Full Text]

26. Nudo RJ, Larson D, Plautz EJ, Friel KM, Barbay S, Frost SB. A squirrel monkey model of poststroke motor recovery. Ilar J. 2003; 44: 161–174.[Medline] [Order article via Infotrieve]

27. van Beek J, Elward K, Gasque P. Activation of complement in the central nervous system: roles in neurodegeneration and neuroprotection. Ann N Y Acad Sci. 2003; 992: 56–71.[Medline] [Order article via Infotrieve]

This article has been cited by other articles:

				U. Ito, Y. Hakamata, E. Kawakami, and K. Oyanagi Degeneration of Astrocytic Processes and Their Mitochondria in Cerebral Cortical Regions Peripheral to the Cortical Infarction: Heterogeneity of Their Disintegration Is Closely Associated With Disseminated Selective Neuronal Necrosis and Maturation of Injury Stroke, June 1, 2009; 40(6): 2173 - 2181. [Abstract] [Full Text] [PDF]

				C. E. Brown and T. H. Murphy Livin' on the Edge: Imaging Dendritic Spine Turnover in the Peri-Infarct Zone during Ischemic Stroke and Recovery Neuroscientist, April 1, 2008; 14(2): 139 - 146. [Abstract] [PDF]

				C. Giffard, B. Landeau, N. Kerrouche, A. R. Young, L. Barre, and J.-C. Baron Decreased Chronic-Stage Cortical 11C-Flumazenil Binding After Focal Ischemia-Reperfusion in Baboons: A Marker of Selective Neuronal Loss? Stroke, March 1, 2008; 39(3): 991 - 999. [Abstract] [Full Text] [PDF]

				U. Ito, J. Nagasao, E. Kawakami, and K. Oyanagi Fate of Disseminated Dead Neurons in the Cortical Ischemic Penumbra: Ultrastructure Indicating a Novel Scavenger Mechanism of Microglia and Astrocytes Stroke, September 1, 2007; 38(9): 2577 - 2583. [Abstract] [Full Text] [PDF]

				C. E. Brown, P. Li, J. D. Boyd, K. R. Delaney, and T. H. Murphy Extensive Turnover of Dendritic Spines and Vascular Remodeling in Cortical Tissues Recovering from Stroke J. Neurosci., April 11, 2007; 27(15): 4101 - 4109. [Abstract] [Full Text] [PDF]

This Article Abstract Full Text (PDF) All Versions of this Article: 37/8/2134    most recent 01.STR.0000231875.96714.b1v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ito, U. Articles by Oyanagi, K. Search for Related Content PubMed PubMed Citation Articles by Ito, U. Articles by Oyanagi, K. Related Collections Animal models of human disease Apoptosis Exercise/exercise testing/rehabilitation Acute Cerebral Infarction