on February 17, 2005
Published online before print February 17, 2005, doi: 10.1161/01.STR.0000157598.02014.37
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Submitted on August 24, 2004
From the Departments of Neurology (A. Slowik, A.B., W.T., J.P., A.T.D., A. Szczudlik) and Neurosurgery (M.B., T.K., R.C.), Jagiellonian University, Krakow, Poland; and the Department of Neurology (D.A.F.), University of Michigan, Ann Arbor. * To whom correspondence should be addressed. E-mail: slowik{at}neuro.cm-uj.krakow.pl.
Background and Purpose--The member 3 of clade A of serine proteinase inhibitors (SERPINA3), known previously as the Methods--A total of 180 patients with aneurysmal SAH and 263 healthy controls were genotyped for the SERPINA3 A/T polymorphism. Aneurysmal SAH was diagnosed by cranial computed tomography or lumbar puncture and digital subtraction angiography. SERPINA3 polymorphism was detected by polymerase chain reaction amplification and restriction enzyme digestion. Results--The SERPINA3 genotype distribution in patients with aneurysmal SAH (AA-29 16.1%; AT-108 60.0%; TT-43 23.9%) differed significantly from controls (AA-70 26.6%; AT-123 46.8%; TT-70 26.6%; P=0.009). A logistic regression model showed that the presence of genotype with T allele (AT+TT; odds ratio [OR], 2.01; 95% CI, 1.19 to 3.38; P=0.009) or AA genotype (OR, 0.49; 95% CI, 0.30 to 0.84; P=0.009) of the SERPINA3 influences the risk for aneurysmal SAH independently from smoking, excessive alcohol consumption, and hypertension. Conclusion--The A/T polymorphism of SERPINA3 gene is associated with the risk factor for aneurysmal SAH.
Revised on December 4, 2004
Accepted on December 16, 2004
Agnieszka Slowik MD*; 
1-Antichymotrypsin Gene (SERPINA3) A/T Polymorphism as a Risk Factor for Aneurysmal Subarachnoid Hemorrhage
1-antichymotrypsin, is an acute phase protein, the levels of which increase in acute and chronic inflammation. The A/T polymorphism of the SERPINA3 gene influences expression of SERPINA3 protein. SERPINA3 can be related to aneurysmal subarachnoid hemorrhage (SAH) by influencing inflammation or by regulating cathepsin G activity. We studied the significance of SERPINA3 A/T polymorphism in patients with aneurysmal SAH compared with healthy controls.
Key words: alpha 1-antichymotrypsin • polymorphism • SERPINA3 • subarachnoid hemorrhage