Stroke, Vol 12, 93-97, Copyright © 1981 by American Heart Association
JM Hallenbeck, TW Furlow Jr and HR Gralnick
To test the hypothesis that plasma contains native constituents capable of
impairing microcirculatory flow in zones of acute ischemic tissue damage,
we performed 14C-antipyrine autoradiographic blood flow studies in
splenectomized dogs subjected to 35 min of cerebrospinal fluid compression
ischemia followed by 30 min of recirculation to the neuraxis. The animals
were anticoagulated with heparin and were divided into 4 groups by exposure
to various measures before induction of ischemia. Groups 1 and 2 served for
comparison with the other groups and underwent, respectively, no glass-wool
filtration and glass-wool filtration via an arteriovenous shunt.
Post-ischemic brain blood flows in Group 1 were low and focal zones of
greatly impaired reperfusion were present. In Group 2, post-ischemic brain
blood flows were high and focal perfusion impairment did not occur. Group 3
received homologous purified factor VIII/von Willebrand factor protein (F
VIII/vWF) after glass-wool filtration but before induction of ischemia;
Group 4 received F VIII/vWF-poor cryoprecipitate at the same time point.
The purpose of administering the plasma preparations was to check for the
presence of activity that nullified the enhancement of post-ischemic
reperfusion expected after exposure to glass-wool. The results indicate
that activity deleterious to post-ischemic reperfusion primarily resides in
the F VIII/vWF fraction of cryoprecipitate. The F VIII/vWF- poor
cryoprecipitate infusate, containing 250 to 800-fold more protein than the
F VIII/vWF fraction, produced an intermediate reduction of blood flow.
ARTICLES
Influence of factor VIII/von Willebrand factor protein (F VIII/vWF) and F VIII/vWF-poor cryoprecipitate on post-ischemic microvascular reperfusion in the central nervous system