Stroke, Vol 17, 738-743, Copyright © 1986 by American Heart Association
ST Chen, CY Hsu, EL Hogan, H Maricq and JD Balentine
In the search for a more reproducible focal ischemic stroke model in the
rat, we systematically interrupted blood flow to the right middle cerebral
artery territory by ligating the right middle cerebral artery, and the
right and left common carotid arteries in succession. Using a laser-Doppler
flowmeter, we found that the relative surface blood flow in cerebral cortex
supplied by the right middle cerebral artery decreased to 62, 48, and 18%
of baseline respectively after successive ligation of the right middle
cerebral artery, and the right and left common carotid arteries. A focal
infarct in the cerebral cortex supplied by the right middle cerebral artery
was consistently noted after ligation of the right middle cerebral and the
right common carotid arteries and temporary clip occlusion of the left
common carotid artery for 60 min. The surface areas of infarction measured
100 +/- 6 mm2 and the maximal cross-sectional area of infarction was 10.4
+/- 1.1 mm2 (N = 10). The mortality rate was 7% (N = 70). The
characteristic changes of ischemic necrosis were limited to the cortex with
sparing of subcortical structures. No motor deficits occurred. Occlusion of
the right middle cerebral artery alone or together with the right common
carotid artery did not consistently cause gross infarction and the maximal
cross-sectional area of infarction was smaller (the right middle cerebral
artery, 1.7 +/- 0.8 mm2, N = 10; the right middle cerebral artery plus the
right common carotid artery, 4.8 +/- 1.9 mm2, N = 10). Permanent ligation
of the right middle cerebral artery and both common carotid arteries had a
high mortality (60% in 3 days, N = 10).(ABSTRACT TRUNCATED AT 250 WORDS)
ARTICLES
A model of focal ischemic stroke in the rat: reproducible extensive cortical infarction
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