This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Takeo, S. Articles by Fujimori, K. Search for Related Content PubMed PubMed Citation Articles by Takeo, S. Articles by Fujimori, K.

Stroke, Vol 23, 62-68, Copyright © 1992 by American Heart Association

ARTICLES

Sustained damage to energy metabolism of brain regions after microsphere embolism in rats

S Takeo, T Taguchi, K Tanonaka, K Miyake, T Horiguchi, N Takagi and K Fujimori
Department of Pharmacology, Tokyo College of Pharmacy, Japan.

BACKGROUND AND PURPOSE: Information on sustained damage to cerebral function and metabolism after cerebral ischemia is useful for prophylaxis and therapeutics of cerebral infarction. The purpose of the present study was to induce sustained damage to brain regions after cerebral ischemia in experimental animals. For this purpose, we examined animal behavior and cerebral energy metabolism following microsphere embolism in rats. METHODS: We injected 900 microspheres (48 microns in diameter) into the right internal carotid artery of 110 rats and determined the time course of changes in the rats' behavior and the energy metabolism of the cortex, striatum, and hippocampus of both hemispheres. We injected the same volume of vehicle, without microspheres, into 28 sham-operated rats; there were 14 nonoperated control rats. RESULTS: Peak increase in lactate content and decrease in adenosine triphosphate and creatine phosphate of these brain regions of the right hemisphere were seen on the first day after microsphere embolism, whereas peak increases in glucose and glycogen contents of these regions were observed on the third day. Most of the metabolic alterations in all these regions continued for up to 28 days after operation, although they recovered toward control levels with time after the operation. The extent and trend of metabolite changes of the right hemisphere after microsphere embolism were similar in the three brain regions. In the left hemisphere, similar metabolic changes were observed, but to a lesser degree. The time course of changes in behavioral scores following microsphere embolism revealed marked stroke- like symptoms on the first day and relatively rapid disappearance of the symptoms with time after embolism. CONCLUSIONS: Microsphere embolism is capable of inducing widespread, sustained damage to energy metabolism of brain regions.

This article has been cited by other articles:

				F. Han, Y. Shirasaki, and K. Fukunaga 3-[2-[4-(3-Chloro-2-methylphenylmethyl)-1-piperazinyl]ethyl]-5,6-dimethoxy-1-(4-imidazolylmethyl)-1H-indazole Dihydro-chloride 3.5 Hydrate (DY-9760e) Is Neuroprotective in Rat Microsphere Embolism: Role of the Cross-Talk between Calpain and Caspase-3 through Calpastatin J. Pharmacol. Exp. Ther., May 1, 2006; 317(2): 529 - 536. [Abstract] [Full Text] [PDF]

				S. Takeo, T. Fukatsu, K. Miyake-Takagi, N. Takagi, M. Niimura, A. Nagakura, T. Ando, and K. Tanonaka Persistent Effects of Delayed Treatment with Nefiracetam on the Water Maze Task in Rats with Sustained Cerebral Ischemia J. Pharmacol. Exp. Ther., February 1, 2003; 304(2): 513 - 523. [Abstract] [Full Text] [PDF]

				N. Takagi, K. Miyake-Takagi, K. Takagi, H. Tamura, and S. Takeo Altered Extracellular Signal-regulated Kinase Signal Transduction by the Muscarinic Acetylcholine and Metabotropic Glutamate Receptors after Cerebral Ischemia J. Biol. Chem., February 15, 2002; 277(8): 6382 - 6390. [Abstract] [Full Text] [PDF]

				N. Takagi, H. Tsuru, M. Yamamura, and S. Takeo Changes in Striatal Dopamine Metabolism After Microsphere Embolism in Rats Stroke, June 1, 1995; 26(6): 1101 - 1106. [Abstract] [Full Text]