This Article Full Text Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Shirotani, T. Articles by Chigasaki, H. Search for Related Content PubMed PubMed Citation Articles by Shirotani, T. Articles by Chigasaki, H.

(Stroke. 1995;26:878-884.)
© 1995 American Heart Association, Inc.

Articles

In Vivo Studies of Extracellular Metabolites in the Striatum After Distal Middle Cerebral Artery Occlusion in Stroke-Prone Spontaneously Hypertensive Rats

Toshiki Shirotani, MD; Katsuji Shima, MD Hiroo Chigasaki, MD

From the Department of Neurosurgery, National Defense Medical College, Tokorozawa, Japan.

Correspondence to Toshiki Shirotani, MD, Department of Neurosurgery, National Defense Medical College, 3-2, Namiki, Tokorozawa, Saitama 359, Japan.

Background and Purpose We demonstrated in a previous study that ⁴⁵Ca accumulation in the lateral part of the striatum was detected 3 days after distal middle cerebral artery (MCA) occlusion using a ⁴⁵Ca autoradiographic technique in stroke-prone spontaneously hypertensive rats. However, the mechanism of delayed neuronal damage that occurred in the lateral part of the striatum is unknown. We examined changes in amino acids and monoamines in the striatums of rat brains after MCA occlusion in stroke-prone spontaneously hypertensive rats using an in vivo brain microdialysis technique.

Methods Microdialysis probes were inserted into the lateral or medial part of the striatum 24 hours before the experiment. The dialysis probe was perfused continuously at 2 µL/min with Ringer's solution, and the dialysate samples were collected every 20 minutes. After a 3-hour period for baseline stabilization, the right MCA was occluded. The dialysate count of monoamines and amino acids was determined by high-performance liquid chromatography.

Results After MCA occlusion, a threefold transient increase in glutamate was observed in the lateral part of the striatum. The level returned to its baseline value 60 minutes after MCA occlusion. Dopamine in the lateral part increased twofold to its peak value. This release persisted for 2 hours after MCA occlusion. There were no significant changes in these components in the extracellular fluid of the medial part of the striatum.

Conclusions Our study demonstrated that changes of neurotransmitters in the lateral part of the striatum after MCA occlusion differed from those in the medial part. These results suggest that excessive release of glutamate and dopamine is related to delayed neuronal damage that occurs in the lateral part of the striatum in this model.

Key Words: dopamine • glutamates • middle cerebral artery occlusion • rats

This article has been cited by other articles:

				A. Melani, L. Pantoni, C. Corsi, L. Bianchi, A. Monopoli, R. Bertorelli, G. Pepeu, F. Pedata, and D. K. J. E. von Lubitz Striatal Outflow of Adenosine, Excitatory Amino Acids, {gamma}-Aminobutyric Acid, and Taurine in Awake Freely Moving Rats After Middle Cerebral Artery Occlusion : Correlations With Neurological Deficit and Histopathological Damage • Editorial Comment: Correlations With Neurological Deficit and Histopathological Damage Stroke, November 1, 1999; 30(11): 2448 - 2455. [Abstract] [Full Text] [PDF]