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Articles

Activated Microglial Cells Are Colocalized With Perivascular Deposits of Amyloid-ß Protein in Alzheimer's Disease Brain

Toshiki Uchihara, MD, PhD; Haruhiko Akiyama, MD, PhD; Hiromi Kondo; Kenji Ikeda, MD, PhD

From the Department of Neurology, Tokyo (Japan) Medical and Dental University (T.U.), and Department of Neuropathology, Tokyo Institute of Psychiatry (T.U., H.A., H.K., K.I.).

Correspondence to T. Uchihara, MD, PhD, Department of Neurology, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113 Japan. E-mail t-uchihara.nuro{at}med.tmd.ac.jp

Background and Purpose Microglial cells are present in the center of senile plaques (SPs) in Alzheimer's disease (AD) brain. Such a localization of microglial cells suggests that they are involved in the deposition or the clearance of amyloid-ß protein (Aß) in the brain. We examined their association with another type of parenchymal Aß deposit, which is termed the perivascular deposits of Aß (PAß).

Methods Thick sections from AD brain were stained with a three-color immunofluorescence method that labeled Aß, activated microglial cells, and vascular endothelial cells simultaneously.

Results Three-dimensional observation under a laser scanning microscope confirmed that perivascular aggregates of activated microglial cells were colocalized with PAß.

Conclusions Microglia occur in association with both SPs and PAß, suggesting that they play important roles in the metabolism of Aß in AD brain.

Key Words: Alzheimer's disease • microscopy, confocal • microglia

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