(Stroke. 1997;28:2053-2059.)
© 1997 American Heart Association, Inc.
Articles |
Extracellular Nucleotide–Induced [Ca2+]i Elevation in Rat Basilar Smooth Muscle Cells
From the Section of Neurosurgery, Department of Surgery, University of Chicago (Ill).
Correspondence to H. Zhang, MD, PhD, Deborah Research Institute, 20 Pine Mill Rd, Brown Mills, NJ 08015-1799. E-mail jzhang{at}cybernet.net
Background and Purpose Extracellular nucleotides play an important role in the regulation of vascular tone and may be involved in cerebral vasospasm after subarachnoid hemorrhage. The objective of this study was to investigate the receptor subtypes for nucleotides and their mechanisms of [Ca2+]i mobilization in cerebral vasculature.
Methods Rat basilar smooth muscle cells were isolated by an enzymatic method. [Ca2+]i mobilization in freshly isolated cells was monitored using fura 2 microfluorimetry.
Result Extracellular nucleotides produced a
concentration-dependent biphasic [Ca2+]i
response, a large transient peak followed by a slowly decaying plateau.
The potency of nucleotides to raise
[Ca2+]i was ATP
S
UDPATP
UDPTTP,
indicating that P2u receptors were expressed in the rat
basilar smooth muscle cells. The effect of UTP to release
Ca2+ from internal stores was reduced by pertussis toxin,
by the phospholipase C inhibitor 2-nitro-4-carboxyphenyl
N,N-diphenylcarbamate, and by the Ca2+-pump
inhibitor thapsigargin. The Ca2+ entry induced
by UTP was partially attenuated by the receptor-operated
Ca2+ channel blocker SK&F96365 and by the voltage-dependent
Ca2+ channel blocker verapamil. P2
receptor antagonists suramin and, at higher concentrations,
pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid reduced the
effect of UTP.
Conclusions The results are the first demonstration that nucleotides activate G protein–coupled P2u receptors to mobilize [Ca2+]i in rat basilar smooth muscle cells.
Key Words: rats • nucleotides • muscle, smooth
This article has been cited by other articles:
 |
|
 |
|
  J. Nilsson, L. M. Nilsson, Y.-W. Chen, J. D. Molkentin, D. Erlinge, and M. F. Gomez High Glucose Activates Nuclear Factor of Activated T Cells in Native Vascular Smooth Muscle Arterioscler Thromb Vasc Biol, April 1, 2006; 26(4): 794 - 800. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. del Valle-Rodriguez, E. Calderon, M. Ruiz, A. Ordonez, J. Lopez-Barneo, and J. Urena Metabotropic Ca2+ channel-induced Ca2+ release and ATP-dependent facilitation of arterial myocyte contraction. PNAS, March 14, 2006; 103(11): 4316 - 4321. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Miyagi and J. H. Zhang {alpha},{beta}-Methylene ATP enhances P2Y4 contraction of rabbit basilar artery Am J Physiol Heart Circ Physiol, April 1, 2004; 286(4): H1546 - H1551. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Miao, Y. Dai, and J. Zhang Mechanism of RhoA/Rho kinase activation in endothelin-1- induced contraction in rabbit basilar artery Am J Physiol Heart Circ Physiol, September 1, 2002; 283(3): H983 - H989. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. G. Welsh and J. E. Brayden Mechanisms of coronary artery depolarization by uridine triphosphate Am J Physiol Heart Circ Physiol, June 1, 2001; 280(6): H2545 - H2553. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. C. Carpenter, L. Miao, Y. Miyagi, E. Bengten, J. H. Zhang, and J. P. Muizelaar Altered Expression of P2 Receptor mRNAs in the Basilar Artery in a Rat Double Hemorrhage Model Editorial Comment Stroke, February 1, 2001; 32(2): 516 - 522. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. Jaggar and M. T. Nelson Differential regulation of Ca2+ sparks and Ca2+ waves by UTP in rat cerebral artery smooth muscle cells Am J Physiol Cell Physiol, November 1, 2000; 279(5): C1528 - C1539. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Aoki, A. Y. Zubkov, A. D. Parent, J. H. Zhang, and R. L. Macdonald Mechanism of ATP-Induced [Ca2+]i Mobilization in Rat Basilar Smooth Muscle Cells Editorial Comment Stroke, June 1, 2000; 31(6): 1377 - 1385. [Abstract] [Full Text] [PDF]
|
|