From the Departments of Neuroanatomy (H.-J.B., A.S., K.Z.) and Neurology
(O.W.W.), Heinrich-Heine-University, Düsseldorf, Germany; and Department
of Biochemistry (K.K.), Aichi Human Service Center, Kamiya, Kasugai, Japan.
Correspondence to Hans-J. Bidmon, Department of Neuroanatomy, Bldg. 22.03.05 Heinrich-Heine-University, PO Box 10 10 07, Moorenstrasse 5, D-40225 Düsseldorf, Germany. E-mail hjb{at}hirn.uni-duesseldorf.de
Background and Purpose—Free radicals
including superoxide are responsible for postlesional cytotoxicity. In
contrast to the constitutive CuZn–superoxide dismutases (SODs),
manganese–superoxide dismutase (Mn–SOD) is inducible and has the
potential to protect neurons by its superoxide dismutating activity.
Therefore, we studied the presence and the regional changes in Mn–SOD
within the brain after focal cortical ischemia.
Methods—Focal cortical photothrombotic lesions were produced in
the hindlimb region of rat brains. Animals were anesthetized
and transcardially perfused with Zamboni's fixative. Mn–SOD was
immunohistochemically localized using an antiserum against rat-Mn–SOD.
Changes in Mn–SOD immunoreactivity were quantified by image
analysis.
Results—Focal photothrombosis caused a perilesional increase in
Mn–SOD after 24 hours, followed by a further significant increase at
48 hours in perilesional cortex, ipsilateral corpus callosum,
hippocampus, and thalamus, as well as in a homotopic cortical area
within the nonlesioned hemisphere. At day 2, Mn–SOD was present in
neurons and astrocytes. Up to day 7, Mn–SOD increased in the entire
ipsilateral and contralateral cortex but remained higher elevated in
the ipsilateral hippocampus and thalamus. Thereafter, Mn–SOD decreased
globally but remained elevated in some cortical neurons up to day
60.
Conclusions—The early transient increase of Mn–SOD in distinct
brain regions, which are functionally connected via afferents and
efferents, suggests that these regions are affected by the injury. It
suggests that Mn–SOD protects the cells in these regions from
superoxide-induced damage and therefore may limit the retrograde and
anterograde spread of neurotoxicity.
Department
of Anesthesiology/,
Critical Care Medicine,
The Johns Hopkins University,
School of Medicine,
Baltimore, Maryland
© 1998 American Heart Association, Inc.
Original Contributions
Transient Increase of Manganese–Superoxide Dismutase in Remote Brain Areas After Focal Photothrombotic Cortical Lesion
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