This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kitazono, T. Articles by Faraci, F. M. Search for Related Content PubMed PubMed Citation Articles by Kitazono, T. Articles by Faraci, F. M.

(Stroke. 1998;29:494-498.)
© 1998 American Heart Association, Inc.

Original Contributions

Role of Tyrosine Kinase in Serotonin-Induced Constriction of the Basilar Artery In Vivo

Takanari Kitazono, MD; Setsuro Ibayashi, MD; Tetsuhiko Nagao, MD; Tomoko Kagiyama, MD; Jiro Kitayama, MD; Masatoshi Fujishima, MD

From the Second Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.

Correspondence to Takanari Kitazono, MD, Second Department of Internal Medicine, Faculty of Medicine, Kyushu University, Maidashi 3–1-1, Higashi-ku, Fukuoka 812, Japan. E-mail kitazono{at}intmed2.med.kyushu-u.ac.jp

Background and Purpose—Serotonin is one of the most potent constrictors of cerebral blood vessels and is implicated in several pathological conditions, including migraine and cerebral ischemia. Recent evidence has suggested that tyrosine kinase is involved in vasocontractile responses. The objective of this study was to test the hypothesis that activation of tyrosine kinase contributes to serotonin-induced constriction of the basilar artery in vivo.

Methods—Using a cranial window in anesthetized Sprague-Dawley rats, we examined effects of inhibitors of tyrosine kinase and tyrosine phosphatase on constrictor responses of the basilar artery to serotonin in vivo.

Results—Serotonin (10^-8, 10^-7, and 10^-6 mol/L) produced constriction of the basilar artery by 12±2%, 27±2%, and 37±3%, respectively. Genistein (3x10^-6 mol/L), an inhibitor of tyrosine kinase, did not affect baseline diameter of the basilar artery but attenuated serotonin-induced vasoconstriction (P<.05 versus control responses). Daidzein, an inactive analogue of genistein, did not affect serotonin-induced constriction of the basilar artery. Tyrphostin 47 (10^-5 mol/L), another inhibitor of tyrosine kinase, also attenuated serotonin-induced vasoconstriction, and tyrphostin 63, an inactive analogue of tyrphostin 47, did not affect the vasoconstriction. Sodium orthovanadate (10^-5 mol/L), an inhibitor of tyrosine phosphatase, enhanced serotonin-induced vasoconstriction. Phorbol 12,13-dibutyrate, a direct activator of protein kinase C, also caused constriction of the basilar artery, which was not affected by genistein or sodium orthovanadate.

Conclusions—These results suggest that serotonin-induced constriction of the basilar artery is mediated, at least in part, by activation of tyrosine kinase in vivo.

Editorial Comment

Frank M. Faraci, PhD, Guest Editor

Department of Internal Medicine, Cardiovascular Division, University of Iowa College of Medicine, Iowa City, Iowa

This article has been cited by other articles:

				P. A. Engel New onset migraine associated with use of soy isoflavone supplements Neurology, October 22, 2002; 59(8): 1289 - 1290. [Full Text] [PDF]

				J. Kitayama, T. Kitazono, S. Ibayashi, M. Wakisaka, Y. Watanabe, M. Kamouchi, T. Nagao, M. Fujishima, and F. M. Faraci Role of Phosphatidylinositol 3-Kinase in Acetylcholine-Induced Dilatation of Rat Basilar Artery Editorial Comment Stroke, October 1, 2000; 31(10): 2487 - 2493. [Abstract] [Full Text] [PDF]

				A. Grumann-Júnior, M. A. Dias, R. V. Alves, J. E. Boteon, and J. B. Calixto Mechanisms Mediating Substance P-Induced Contraction in the Rat Iris In Vitro Invest. Ophthalmol. Vis. Sci., June 1, 2000; 41(7): 1861 - 1870. [Abstract] [Full Text]

				J. A. Madden and N. J. T. Christman Integrin signaling, free radicals, and tyrosine kinase mediate flow constriction in isolated cerebral arteries Am J Physiol Heart Circ Physiol, December 1, 1999; 277(6): H2264 - H2271. [Abstract] [Full Text] [PDF]

				S. J. Elliott, D. J. Lacey, W. M. Chilian, and A. K. Brzezinska Peroxynitrite is a contractile agonist of cerebral artery smooth muscle cells Am J Physiol Heart Circ Physiol, November 1, 1998; 275(5): H1585 - H1591. [Abstract] [Full Text] [PDF]