This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nomura, H. Articles by Macdonald, R. L. Search for Related Content PubMed PubMed Citation Articles by Nomura, H. Articles by Macdonald, R. L.

(Stroke. 1998;29:1014-1019.)
© 1998 American Heart Association, Inc.

Original Contributions

Anticardiolipin Antibody Aggravates Cerebral Vasospasm After Subarachnoid Hemorrhage in Rabbits

Hiroaki Nomura, MD; Yutaka Hirashima, MD; Shunro Endo, MD; Akira Takaku, MD

From the Department of Neurosurgery, Toyama Medical and Pharmaceutical University, Toyama, Japan.

Correspondence to Hiroaki Nomura, MD, Department of Neurosurgery, Toyama Medical and Pharmaceutical University, Sugitani 2630, Toyama, Toyama 930–01, Japan.

Background and Purpose—We previously reported that patients with antiphospholipid antibodies (aPLs) frequently demonstrate cerebral infarction due to cerebral vasospasm after subarachnoid hemorrhage (SAH). To examine the participation of aPLs in the pathogenesis of vasospasm after SAH, we studied the relationships of aPLs and SAH in an animal model.

Methods—SAH was produced in 34 rabbits that received two subarachnoid injections of autologous arterial blood. The animals were divided into four experimental groups: SAH was induced in group A (n=9), intracutaneous injection of cardiolipin (CL) was performed before the induction of SAH in group B (n=5), intravenous injection of CL was performed before SAH in group C (n=12), and cyclosporin A was infused intravenously after the intravenous injection of CL and induction of SAH in group D (n=8). Enzyme-linked immunosorbent assay identifying the titer of IgG CL antibodies, neurological evaluation, cerebral angiography, and histological examination were performed in all four groups.

Results—A significant elevation of anti-CL antibodies, aggravation of neurological deficit, and reduction of caliber of the basilar artery were observed in rabbits that received the intravenous immunization of CL (group C). The administration of cyclosporin A reduced the titer of anti-CL antibody, aggravation of neurological deficit, constriction of basilar artery, and the incidence of cerebral infarction (group D).

Conclusions—Anti-CL antibodies may therefore be involved in the deterioration of cerebral vasospasm after SAH.

Editorial Comment

R. Loch Macdonald, MD, PhD, Guest Editor

Section of Neurosurgery, University of Chicago Medical Center, Chicago, Illinois

This article has been cited by other articles:

				M. M. Firat, V. Gelebek, H. S. Orer, D. Belen, A. K. Firat, and F. Balkanci Selective Intraarterial Nimodipine Treatment in an Experimental Subarachnoid Hemorrhage Model AJNR Am. J. Neuroradiol., June 1, 2005; 26(6): 1357 - 1362. [Abstract] [Full Text] [PDF]

				K. Toyoda, F. M. Faraci, Y. Watanabe, T. Ueda, J. J. Andresen, Y. Chu, S. Otake, and D. D. Heistad Gene Transfer of Calcitonin Gene-Related Peptide Prevents Vasoconstriction After Subarachnoid Hemorrhage Circ. Res., October 27, 2000; 87(9): 818 - 824. [Abstract] [Full Text] [PDF]