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(Stroke. 2005;36:654.)
© 2005 American Heart Association, Inc.

Original Contributions

Inosine Reduces Ischemic Brain Injury in Rats

Hui Shen, MD; Guann-Juh Chen, MD; Brandon K. Harvey, PhD; Paula C. Bickford, PhD Yun Wang, MD, PhD

From the National Institute on Drug Abuse (H.S., G.-J.C., B.K.H., P.C.B., Y.W.), National Institutes of Health, Baltimore, Md; and the Center for Aging and Brain Repair (B.K.H., P.C.B.), University of South Florida, Tampa, Fla.

Correspondence to Dr Yun Wang, National Institute on Drug Abuse, IRP, 5500 Nathan Shock Dr, Baltimore, MD 21224. E-mail ywang{at}intra.nida.nih.gov

Background and Purpose— Purinergic nucleoside inosine elicits protection and regeneration during various injuries. The purpose of this study was to examine the protective effects of inosine against cerebral ischemia.

Methods— Adult Sprague-Dawley rats were anesthetized. Inosine, hypoxathine, or vehicle was administered intracerebroventricularly before transient right middle cerebral artery occlusion (MCAo). Animals were placed in behavioral chambers 2 days to 2 weeks after MCAo and then euthanized for tri-phenyl-tetrazolium chloride staining. Glutamate release was measured by microdialysis/high-performance liquid chromatography, and single-unit action potentials were recorded from neurons in the parietal cortex.

Results— Stroke animals receiving inosine pretreatment demonstrated a higher level of locomotor activity and less cerebral infarction. Intracerebroventricular administration of the same dose of hypoxanthine did not confer protection. Coadministration of selective A3 receptor antagonist 3-ethyl-5-benzyl-2-methyl-4-phenylethynyl-6-phenyl-1, 4-(±)-dihydropyridine-3,5-dicarboxylate (MRS1191) significantly reduced inosine-mediated protection. Inosine did not alter basal glutamate release, nor did it reduce ischemia-evoked glutamate overflow from cerebral cortex. However, inosine antagonized glutamate-induced electrophysiological excitation in cerebral cortical neurons.

Conclusions— Inosine inhibits glutamate postsynaptic responses and reduces cerebral infarction. Its protective effect against ischemia/reperfusion-related insults may involve activation of adenosine A3 receptors.

Key Words: inosine, adenosine • neuroprotection • stroke

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