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(Stroke. 2007;38:2861.)
© 2007 American Heart Association, Inc.

Research Letters

A New Hippocampal Model for Examining Intracerebral Hemorrhage-Related Neuronal Death

Effects of Deferoxamine on Hemoglobin-Induced Neuronal Death

Shuijiang Song, MD; Ya Hua, MD; Richard F. Keep, PhD; Julian T. Hoff, MD Guohua Xi, MD

From the Departments of Neurosurgery (S.S., Y.H., R.F.K., J.T.H., G.X.) and Physiology (R.F.K.), University of Michigan, Ann Arbor.

Correspondence to Guohua Xi, MD, R5018 Biomedical Science Research Building, University of Michigan, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200. E-mail guohuaxi{at}umich.edu

Abstract

Background and Purpose— There is an urgent need to develop a model in which to study the mechanism of intracerebral hemorrhage-induced neuronal death in vivo.

Methods— This study was divided into 2 parts: (1) Rats received either an infusion of hemoglobin, ferrous iron, or saline into the right hippocampus; (2) Rats had an infusion of hemoglobin and then were treated with either deferoxamine or vehicle. Rats were killed for hippocampus size, DNA damage, and neuronal death measurements.

Results— Compared with saline, hemoglobin or iron injection caused hippocampal neuronal death. Systemic use of deferoxamine reduced hemoglobin-induced DNA damage, hippocampal neuronal death, and atrophy.

Conclusions— This article demonstrates a new model and indicates that iron has a key role in hemoglobin–induced neuronal death.

Key Words: cerebral hemorrhage • hemoglobin • iron • neuronal death • deferoxamine