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(Stroke. 2008;39:943.)
© 2008 American Heart Association, Inc.

Original Contributions

Inflammatory and Injury Responses to Ischemic Stroke in Obese Mice

Satoshi Terao, MD; Gokhan Yilmaz, MD; Karen Y. Stokes, PhD; Mami Ishikawa, MD, PhD; Takeshi Kawase, MD, PhD D. Neil Granger, PhD

From the Department of Molecular and Cellular Physiology (S.T., G.Y., K.Y.S., D.N.G.), Louisiana State University Health Sciences Center, Shreveport, La; the Department of Neurosurgery (M.I.), Jichi Medical School Hospital, Jichi, Japan; and the Department of Neurosurgery (T.K.), Keio University Hospital, Keio, Japan.

Correspondence to D. Neil Granger, PhD, Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, 1501 Kings Highway, Shreveport, LA 71130. E-mail dgrang{at}lsuhsc.edu

Background and Purpose— Although epidemiological studies reveal an increased incidence of obesity and an association between obesity and the prevalence/severity of ischemic stroke, little is known about the mechanisms that link obesity to ischemic stroke. This study tested the hypothesis that obesity exacerbates the cerebrovascular dysfunction and tissue injury induced by brain ischemia and reperfusion.

Methods— The adhesion of leukocytes and platelets in cerebral venules, blood–brain barrier permeability, brain water content, and infarct volume were measured in wild-type, obese (ob/ob), and leptin-reconstituted ob/ob mice subjected to 30 minutes middle cerebral artery occlusion and reperfusion. Tissue and plasma cytokine levels were determined by cytometric bead array, and a role for monocyte chemoattractant protein-1 and interleukin-6 was assessed using blocking antibodies.

Results— Compared with wild-type mice, ob/ob exhibited larger increases in leukocyte and platelet adhesion, blood–brain barrier permeability, water content, and infarct volume after middle cerebral artery occlusion–reperfusion. Reconstitution of leptin in ob/ob mice tended to further enhance all reperfusion-induced responses. Ob/ob mice also exhibited higher plasma levels of monocyte chemoattractant protein-1 and interleukin-6 than wild-type mice. Immunoneutralization of monocyte chemoattractant protein-1, but not interleukin-6, reduced infarct volume in ob/ob mice.

Conclusions— Obesity worsens the inflammatory and injury responses to middle cerebral artery occlusion and reperfusion by a mechanism independent of leptin deficiency. monocyte chemoattractant protein-1 appears to contribute to the exaggerated responses to ischemic stroke in obese mice.

Key Words: cerebral infarct • chemokines • cytokines • obesity • platelets

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