Published Online
on August 30, 2007

A more recent version of this article appeared on October 1, 2007

This Article Full Text (PDF) All Versions of this Article: 38/10/2861    most recent STROKEAHA.107.488015v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Song, S. Articles by Xi, G. Search for Related Content PubMed PubMed Citation Articles by Song, S. Articles by Xi, G. Related Collections Acute Cerebral Hemorrhage

Submitted on March 13, 2007
Accepted on April 10, 2007

A New Hippocampal Model for Examining Intracerebral Hemorrhage-Related Neuronal Death. Effects of Deferoxamine on Hemoglobin-Induced Neuronal Death

Shuijiang Song MD; Ya Hua MD; Richard F. Keep PhD; Julian T. Hoff MD; and Guohua Xi MD^*

From the Departments of Neurosurgery (S.S., Y.H., R.F.K., J.T.H., G.X.), and Physiology (R.F.K.), University of Michigan, Ann Arbor.

^* To whom correspondence should be addressed. E-mail: guohuaxi{at}umich.edu.

Background and Purpose—There is an urgent need to develop a model in which to study the mechanism of intracerebral hemorrhage-induced neuronal death in vivo.

Methods—This study was divided into 2 parts: (1) Rats received either an infusion of hemoglobin, ferrous iron, or saline into the right hippocampus; (2) Rats had an infusion of hemoglobin and then were treated with either deferoxamine or vehicle. Rats were killed for hippocampus size, DNA damage, and neuronal death measurements.

Results—Compared with saline, hemoglobin or iron injection caused hippocampal neuronal death. Systemic use of deferoxamine reduced hemoglobin-induced DNA damage, hippocampal neuronal death, and atrophy.

Conclusions—This article demonstrates a new model and indicates that iron has a key role in hemoglobin–induced neuronal death.

Key words: cerebral hemorrhage • hemoglobin • iron • neuronal death • deferoxamine

This article has been cited by other articles:

				Y. Gu, Y. Hua, R. F. Keep, L. B. Morgenstern, and G. Xi Deferoxamine Reduces Intracerebral Hematoma-Induced Iron Accumulation and Neuronal Death in Piglets Stroke, June 1, 2009; 40(6): 2241 - 2243. [Abstract] [Full Text] [PDF]

				M. Okauchi, Y. Hua, R. F. Keep, L. B. Morgenstern, and G. Xi Effects of Deferoxamine on Intracerebral Hemorrhage-Induced Brain Injury in Aged Rats Stroke, May 1, 2009; 40(5): 1858 - 1863. [Abstract] [Full Text] [PDF]