Published Online
on March 13, 2008

A more recent version of this article appeared on May 1, 2008

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Submitted on October 16, 2007
Accepted on October 25, 2007

Middle Cerebral Artery Occlusion in the Rabbit Using Selective Angiography. Application for Assessment of Thrombolysis

Reza Jahan MD^*; Daphne Stewart MD; Harry V. Vinters MD; William Yong MD; Fernando Vinuela MD; Pete Vandeberg MD; and Victor J. Marder MD

From the Departments of Radiological Sciences (R.J., F.V.), Medicine (D.S., V.J.M.), Pathology & Laboratory Medicine (H.V.V., W.Y.), Neurology (H.V.V., V.J.M.), David Geffen School of Medicine at UCLA, Los Angeles, Calif; and R&D BioAnalytics (P.V.), Talecris Biotherapeutics, Research Triangle Park, NC.

^* To whom correspondence should be addressed. E-mail: rjahan{at}mednet.ucla.edu.

Background and Purpose—An animal model of selective middle cerebral artery (MCA) occlusion is needed for evaluation of intra-arterial (IA) delivery of thrombolytic agents. We describe a technique for MCA thrombo-occlusion in the rabbit with real-time angiographic documentation of occlusion and thrombolytic recanalization.

Methods—After femoral artery cutdown, a microcatheter was advanced from the internal carotid artery to the MCA. MCA occlusion was achieved by IA thrombin and reperfusion by IA plasmin.

Results—The terminal internal carotid artery was successfully catheterized in 12 of 13 animals. Stable (2-hour) MCA occlusion was induced and verified angiographically in all 12 animals; 2 animals also had distal internal carotid artery thrombus. Recanalization was achieved rapidly after IA plasmin in 3 of 3 animals.

Conclusions—We describe a new animal model of selective MCA occlusion documented by real-time angiography and used to demonstrate recanalization with IA plasmin.

Key words: animal model • ischemic stroke • middle cerebral artery • thrombolysis

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