on June 5, 2008
Published online before print June 5, 2008, doi: 10.1161/STROKEAHA.107.509927
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Submitted on November 14, 2007
From the Department of Diagnostic Radiology (S.W., C.N.T., P.-L.K.), The University of Hong Kong; the Laboratory of Biomedical Imaging and Signal Processing, Department of Electrical and Electronic Engineering (E.X.W., H.-F.L.), The University of Hong Kong; and the Department of Pediatric and Adolescent Medicine (P.-T.C.), The University of Hong Kong, Hong Kong. * To whom correspondence should be addressed. E-mail: plkhong{at}hkucc.hku.hk.
Background and Purpose—We evaluate white matter (WM) injury after hypoxic-ischemic (HI) insult in a neonatal rat model using diffusion tensor imaging (DTI) to determine whether Methods—Eighteen 7-day-old Sprague-Dawley rats underwent unilateral ligation of left common carotid artery followed by 50 minutes (n=9) or 90 minutes (n=9) of hypoxia at 37°C. Rats were divided into 2 groups, according to absence (group A, n=11) or presence (group B, n=7) of cystic lesions on D7 post-HI T2-weighted imaging. DTI was performed for all rats at D1 and for group A rats at D7 post-HI. Signal intensity of ipsilateral and contralateral external capsule (EC) on D1 was compared by paired t test, with histological correlation. Results—Group A rats had significantly reduced FA, elevated trace, elevated Conclusions—Increased
Accepted on January 3, 2008
Characterization of White Matter Injury in a Hypoxic-Ischemic Neonatal Rat Model by Diffusion Tensor MRI
Silun Wang MPhil;
|| and 
are able to characterize type and severity of brain damage. and similar || on D1 in the ipsilateral compared to contralateral EC, whereas group B rats had significant reduction in all parameters in the ipsilateral EC. Elevated trace normalized on D7 in group A rats. Histopathologic results demonstrated reduced myelination in group A noncystic HI and severe necrosis in group B cystic HI. with no significant change in || appears to characterize noncystic WM injury with reduced myelination, whereas reduction in both || and characterize severe damage with loss of structural integrity and necrosis. Combining with FA and trace, || and provide additional information which reflects type and severity of HI injury.
Key words: MRI • diffusion tensor imaging • white matter • neonatal rat • hypoxic-ischemic
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