on April 16, 2009
Published online before print April 16, 2009, doi: 10.1161/STROKEAHA.108.539536
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Submitted on October 7, 2008
Department of Neurosurgery (Y.G., Y.H., R.F.K., L.B.M., G.X.) and the Stroke Program (R.F.K., L.B.M., G.X.), University of Michigan, Ann Arbor. * To whom correspondence should be addressed. E-mail: guohuaxi{at}umich.edu.
Background and Purpose—Our previous studies found that deferoxamine reduces intracerebral hemorrhage (ICH)-induced brain injury in rats. The current study examined whether deferoxamine reduces brain injury in a piglet ICH model. Methods—Pigs received an injection of autologous blood into the right frontal lobe. Deferoxamine (50 mg/kg, IM) or vehicle was administered 2 hours after ICH and then every 12 hours up to 7 days. Animals were killed 3 or 7 days later to examine iron accumulation, white matter injury, and neuronal death. Results—ICH resulted in development of a reddish perihematomal zone, and iron accumulation, ferritin upregulation, and neuronal death within that zone. Deferoxamine reduced the perihematomal reddish zone, white matter injury, and the number of Perls', ferritin, and Fluoro-Jade C–positive cells. Conclusions—Iron accumulation occurs in the piglet brain after ICH. Deferoxamine reduces ICH-induced iron buildup and brain injury in piglets.
Accepted on November 10, 2008
Deferoxamine Reduces Intracerebral Hematoma-Induced Iron Accumulation and Neuronal Death in Piglets
Yuxiang Gu MD, PhD;
Key words: deferoxamine • intracerebral hematoma • iron • neuronal death